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Cited by 11Spinal Cord Injury ResearchMesenchymal stem cell researchNerve injury and regeneration

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Transplantation of neurotrophin-3-transfected bone marrow mesenchymal stem cells for the repair of spinal cord injury

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Dong, Yuzhen, Yang, Libin, Yang, Lin, Zhao, Hongxing, Zhang, Chao, Wu, Dapeng·2014·Neural Regeneration Research
DOI 10.4103/1673-5374.139478PMC PMC4192969
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Abstract

Bone marrow mesenchymal stem cell transplantation has been shown to be therapeutic in the repair of spinal cord injury. However, the low survival rate of transplanted bone marrow mesenchymal stem cells in vivo remains a problem. Neurotrophin-3 promotes motor neuron survival and it is hypothesized that its transfection can enhance the therapeutic effect. We show that in vitro transfection of neurotrophin-3 gene increases the number of bone marrow mesenchymal stem cells in the region of spinal cord injury. These results indicate that neurotrophin-3 can promote the survival of bone marrow mesenchymal stem cells transplanted into the region of spinal cord injury and potentially enhance the therapeutic effect in the repair of spinal cord injury.

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Figure 1 The number of bone marrow mesenchymal stem cells (BMSCs) transfected with or without neurotrophin-3 (NT-3) during culture. After 3 days, the number of BMSCs transfected with NT-3 was significantly higher than that of non-transfected with NT-3 (P < 0.05). The data were expressed as mean ± SD and analyzed using two-sample t-test.
Figure 1 The number of bone marrow mesenchymal stem cells (BMSCs) transfected with or without neurotrophin-3 (NT-3) during culture. After 3 days, the number of BMSCs transfected with NT-3 was significantly higher than that of non-transfected with NT-3 (P < 0.05). The data were expressed as mean ± SD and analyzed using two-sample t-test.CC BY-NC-SA · PubMed Central
Figure 2 Expression of enhanced green fluorescent protein (EGFP; green fluorescence) in the bone marrow mesenchymal stem cells (BMSCs) transplanted into the injured spinal cord at 4 weeks after transplantation (fluorescence microscope, × 200). EGFP expression was significantly greater in the BMSCs + neurotrophin-3 group (C) than in the spinal cord injury (A) and BMSCs groups (B). The expression was lowest in the spinal cord injury group.
Figure 2 Expression of enhanced green fluorescent protein (EGFP; green fluorescence) in the bone marrow mesenchymal stem cells (BMSCs) transplanted into the injured spinal cord at 4 weeks after transplantation (fluorescence microscope, × 200). EGFP expression was significantly greater in the BMSCs + neurotrophin-3 group (C) than in the spinal cord injury (A) and BMSCs groups (B). The expression was lowest in the spinal cord injury group.CC BY-NC-SA · PubMed Central
Figure 3 Apoptosis of motor neurons in spinal cord anterior horn at 4 weeks after transplantation (TUNEL staining, × 400). (A–C) Spinal cord injury, bone marrow mesenchymal stem cells (BMSCs), and BMSCs + neurotrophin-3 (NT-3) groups. Apoptotic cells in the spinal cord anterior horn exhibited yellow-brown nuclei. The number of apoptotic cells was lowest in the BMSCs + NT-3 group but highest in the spinal cord injury group.
Figure 3 Apoptosis of motor neurons in spinal cord anterior horn at 4 weeks after transplantation (TUNEL staining, × 400). (A–C) Spinal cord injury, bone marrow mesenchymal stem cells (BMSCs), and BMSCs + neurotrophin-3 (NT-3) groups. Apoptotic cells in the spinal cord anterior horn exhibited yellow-brown nuclei. The number of apoptotic cells was lowest in the BMSCs + NT-3 group but highest in the spinal cord injury group.CC BY-NC-SA · PubMed Central

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Research works printed but not linked: 32

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Metadata

Authors
Dong, Y., Yang, L., Yang, L., Zhao, H., Zhang, C., & Wu, D.
Affiliations
First Affiliated Hospital of Xinxiang Medical University (CN)
Corresponding author
Dong, Yuzhen
ORCID
Venue
Neural Regeneration Research
Publisher
Ovid Technologies (Wolters Kluwer Health)
Published
2014
Volume
9
Issue
16
Pages
1520
ISSN
1673-5374 (ISSN-L)
DOI
10.4103/1673-5374.139478
PMC
PMC4192969
OpenAlex
W1985088489
Cited by
11
References
0
Citations by year
2024: 2 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 1 · 2019: 1 · 2017: 2 · 2016: 1 · 2015: 1
FWCI
0.99
Type
journal article
Language
en
Open access
Licence
cc-by-nc-sa
Locations
  • Neural Regeneration Research (publishedVersion, cc-by-nc-sa)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
  • 中国神经再生研究：英文版
Funders
Xinxiang Medical University
Topics
  • Health Sciences › Medicine › Clinical Neurology › Spinal Cord Injury Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Mesenchymal stem cell research
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
Keywords
neurotrophin-3, bone marrow mesenchymal stem cells, spinal cord injury, transfection, stem cell transplantation, stem cell survival, motor neuron survival, functional recovery

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