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Anti-aging potential and phytochemicals of Centella asiatica, Nelumbo nucifera, and Hibiscus sabdariffa extracts
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Centella asiatica, Nelumbo nucifera Gaertn, and Hibiscus sabdariffa have been used as medicinal plants in Thailand. They are sources of phytochemicals that applications for esthetic and healthcare. The aim of this research was to examine the phytochemical constituents and anti-aging potential of these plants. The phytochemical compounds were performed using gas chromatography-mass spectrometry. The anti-aging activities were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azinobis (3-ethylbenzothiazoline-6-sunfonic acid) (ABTS), anti-collagenase, and anti-elastase assays. The main interest phytochemical compounds of ethanolic extracts of C. asiatica, N. nucifera, H. sabdariffa were ethanol, 2-(-Octadecenyloxy), γ-sitosterol and hexadecanoic acid, and ethyl ester, respectively. The DPPH half-maximal inhibitory concentration (IC50) results of C. asiatica, N. nucifera, and H. sabdariffa were 0.32 ± 0.01, 0.34 ± 0.00, and 0.35 ± 0.01 mg/mL, respectively. The ABTS result of H. sabdariffa extract showed high inhibitory activity at IC50of the extract was 0.62 ± 0.12 mg/mL. The percentage of collagenase inhibition of C. asiatica, N. nucifera, and H. sabdariffa at 1.0 mg/mL was 78.13 ± 4.42, 85.94 ± 2.21, and 90.63 ± 0.00, respectively. The C. asiatica extract had a high percentage of elastase inhibition. Consequently, these research results suggest that phytochemicals may also provide a range of esthetic and health benefits. The phytochemical constituent could be used as anti-aging active ingredient for cosmetic and pharmaceutical industrials.
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All three plant extracts (C. asiatica, N. nucifera, and H. sabdariffa) exhibited significant antioxidant activity, with C. asiatica showing the highest activity.
“They found that all three plant extracts exhibited significant antioxidant activity, with C. asiatica showing the highest activity.”
From Cosmeceuticals in photoaging: A review. (2024) · cites this paper as [35]
The Results and Discussion report antioxidant activity for all three extracts. C. asiatica had the highest activity in the DPPH assay, but H. sabdariffa had the highest activity in the ABTS assay. The claim omits that assay-dependent distinction. (LLM, conf=0.90)
Experimental — accuracy not yet measuredThe Khuanekkaphan et al. 35 study level of evidence is considered low according to the Oxford Centre for Evidence-Based Medicine.
“However, the level of evidence for the findings presented in this study is considered low according to the Oxford Centre for Evidence‐Based Medicine.”
From Cosmeceuticals in photoaging: A review. (2024) · cites this paper as [35]
The Materials and Methods sections describe laboratory assays, and the Results and Discussion report antioxidant activity; neither gives an Oxford Centre for Evidence-Based Medicine rating. A search for the Oxford framework and evidence grading found no passage in our copy assessing the study’s level of evidence. (LLM, conf=0.75)
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Metadata
- Authors
- Khuanekkaphan, M., Noysang, C., & Khobjai, W.
- Affiliations
- Rajamangala University of Technology (TH)
- Corresponding author
- Khuanekkaphan, Monsicha
- Venue
- Journal of Advanced Pharmaceutical Technology & Research
- Published
- 2020
- Volume
- 11
- Issue
- 4
- Pages
- 174
- ISSN
- 0976-2094 (ISSN-L)
- 2231-4040
- PMC
- PMC7784944
- OpenAlex
- W3092272909
- Cited by
- 24
- References
- 18
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 6 · 2023: 7 · 2022: 4 · 2021: 1
- FWCI
- 2.19
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by-nc-sa
- Locations
- Journal of Advanced Pharmaceutical Technology amp Research (publishedVersion, cc-by-nc-sa)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Medicinal Plant Research
- Health Sciences › Medicine › Biochemistry (medical) › Phytochemicals and Antioxidant Activities
- Keywords
- Centella asiatica, Nelumbo nucifera, Hibiscus sabdariffa, anti-aging effects, phytochemicals, ethanolic plant extract, antioxidant activity, DPPH radical scavenging, ABTS radical scavenging, collagenase inhibition, elastase inhibition, γ-sitosterol, medicinal plants, cosmetic ingredients
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