Isolation of cholesterol-lowering lactic acid bacteria from human intestine for probiotic use
Open accessAbstract
Cholesterol-lowering effect of lactic acid bacteria (LAB: Streptococcus, Lactobacillus and Bifidobacterium) is well-known. Thus, we investigated LAB isolated from human intestine on the cholesterol-lowering effect in vitro. Seven Streptococcus (61.1%), 11 Lactobacillus (71.8%) and 7 Bifidobacterium (27.9%) were isolated as acid (pH 2.5 and 3.0) and bile (0.3% oxgall) tolerant strains. Streptococcus HJS-1, Lactobacillus HJL-37 and Bifidobacterium HJB-4 were finally selected as probiotic strains to use through the bile salt hydrolase (BSH) activity assay by using MRS media added taurodeoxycholic acid (TDCA) and the cholesterol-lowering test by using soluble cholesterol containing MRS broth. These studies suggested that the isolated LAB had an excellent hypocholesterolemic effect.
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High survival rate of Streptococcus isolates was obtained after testing against these gastrointestinal models.
“Moreover, the high survival rate of Streptococcus isolates was obtained after testing against these gastrointestinal models [50].”
From Bioprophylactic potential of novel human colostrum probiotics via apoptotic induction of colon cancer cells and cell immune activation (2022) · cites this paper as [50]
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- Authors
- Lim, H. J., Kim, S. Y., & Lee, W. K.
- Affiliations
- Chungbuk National University (KR)
- ORCID
- Kim, So Young
- Venue
- Journal of Veterinary Science
- Publisher
- XMLink
- Published
- 2004
- Volume
- 5
- Issue
- 4
- Pages
- 391
- ISSN
- 1229-845X (ISSN-L)
- OpenAlex
- W2223751714
- Cited by
- 94
- References
- 14
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 2 · 2023: 2 · 2022: 7 · 2021: 3 · 2020: 5 · 2019: 2 · 2018: 11 · 2017: 2 · 2016: 2 · 2015: 6 · 2014: 4 · 2013: 5 · 2012: 4
- FWCI
- 4.22
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Journal of Veterinary Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Chungbuk National University
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Steroid Chemistry and Biochemistry
- Keywords
- lactic acid bacteria, cholesterol-lowering effect, hypocholesterolemic activity, probiotic strains, human gut microbiome, bile tolerance, acid resistance, Lactobacillus, Streptococcus, Bifidobacterium, taurodeoxycholic acid
- MeSH terms
- Amidohydrolases, Bifidobacterium, Cholesterol, Feces, Female, Humans, Intestines, Lactobacillus, Male, Streptococcus, Taurodeoxycholic Acid, Probiotics
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