The DOI matches its Crossref record; 40 of the 40 references it cites were found in a registry or an index.
An introduction to the wound healing assay using live-cell microscopy
Open accessAbstract
The wound healing assay is used in a range of disciplines to study the coordinated movement of a cell population. In this technical review, we describe the workflow of the wound healing assay as monitored by optical microscopy. Although the assay is straightforward, a lack of standardization in its application makes it difficult to compare results and reproduce experiments among researchers. We recommend general guidelines for consistency, including: (1) sample preparation including the creation of the gap, (2) microscope equipment requirements, (3) image acquisition, and (4) the use of image analysis to measure the gap size and its rate of closure over time. We also describe parameters that are specific to the particular research question, such as seeding density and matrix coatings. All of these parameters must be carefully controlled within a given set of experiments in order to achieve accurate and reproducible results.
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An image J plugin for the high throughput image analysis of in vitro scratch wound healing assays
(2020) · doi:10.1371/journal.pone.0232565 · Cited by 851
Tutorial: guidance for quantitative confocal microscopy
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Self-citationIn vitro cell migration quantification method for scratch assays
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Human In Vitro Skin Models for Wound Healing and Wound Healing Disorders
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Metadata
- Authors
- Jonkman, J. E. N., Cathcart, J. A., Xu, F., Bartolini, M. E., Amon, J. E., Stevens, K. M., & Colarusso, P.
- Venue
- Cell Adhesion & Migration
- Publisher
- Informa UK Limited
- Published
- 2014-09-03
- Volume
- 8
- Issue
- 5
- Pages
- 440-451
- ISSN
- 1933-6918 (ISSN-L)
- 1933-6926
- OpenAlex
- W2168182874
- Cited by
- 729
- References
- 40
- FWCI
- 8.25
- Type
- journal article
- Language
- en
- Open access
- gold
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- Jonkman, J. E. N., Cathcart, J. A., Xu, F., Bartolini, M. E., Amon, J. E., Stevens, K. M., & Colarusso, P. (2014). An introduction to the wound healing assay using live-cell microscopy. Cell Adhesion & Migration. https://doi.org/10.4161/cam.36224
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