The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Genotoxicity of Escherichia coli Nissle 1917 strain cannot be dissociated from its probiotic activity
Open accessAbstract
Oral administration of the probiotic bacterium Escherichia coli Nissle 1917 improves chronic inflammatory bowel diseases, but the molecular basis for this therapeutic efficacy is unknown. E. coli Nissle 1917 harbors a cluster of genes coding for the biosynthesis of hybrid nonribosomal peptide-polyketide(s). This biosynthetic pathway confers the ability for bacteria to induce DNA double strand breaks in eukaryotic cells. Here we reveal that inactivation of the clbA gene within this genomic island abrogated the ability for the strain to induce DNA damage and chromosomal abnormalities in non-transformed cultured rat intestinal epithelial cells but is required for the probiotic activity of E. coli Nissle 1917. Thus, evaluation of colitis severity induced in rodent fed with E. coli Nissle 1917 or an isogenic non-genotoxic mutant demonstrated the need for a functional biosynthetic pathway both in the amelioration of the disease and in the modulation of cytokine expression. Feeding rodents with a complemented strain for which genotoxicity was restored confirmed that this biosynthetic pathway contributes to the health benefits of the probiotic by modulating its immunomodulatory properties. Our data provide additional evidence for the benefit of this currently used probiotic in colitis but remind us that an efficient probiotic may also have side effects as any other medication.
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DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139
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A microbial symbiosis factor prevents intestinal inflammatory disease
Mazmanian, S. K., Round, J. L., & Kasper, D. L. (2008) · Nature · doi:10.1038/nature07008 · Cited by 2,340
Escherichia coli Induces DNA Double-Strand Breaks in Eukaryotic Cells
Nougayrède, J.-P., Homburg, S., Taieb, F., Boury, M., Brzuszkiewicz, E., Gottschalk, G., Buchrieser, C., Hacker, J., Dobrindt, U., & Oswald, E. (2006) · Science · doi:10.1126/science.1127059 · Cited by 1,188
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Escherichia coli induces DNA damage in vivo and triggers genomic instability in mammalian cells
Cuevas-Ramos, G., Petit, C. R., Marcq, I., Boury, M., Oswald, E., & Nougayrède, J.-P. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1001261107 · Cited by 855
Self-citationThe immune system and the gut microbiota: friends or foes?
Cerf-Bensussan, N., & Gaboriau-Routhiau, V. (2010) · Nature Reviews Immunology · doi:10.1038/nri2850 · Cited by 704
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Probiotic Escherichia coli Nissle 1917 inhibits leaky gut by enhancing mucosal integrity
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Kothari, D., Patel, S., & Kim, S.-K. (2019) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2018.12.104 · Cited by 445
Arthur, J. C., Gharaibeh, R. Z., Mühlbauer, M., Perez-Chanona, E., Uronis, J. M., McCafferty, J., Fodor, A. A., & Jobin, C. (2014) · Nature Communications · doi:10.1038/ncomms5724 · Cited by 392
E. coli as an All-Rounder: The Thin Line Between Commensalism and Pathogenicity
Leimbach, A., Hacker, J., & Dobrindt, U. (2013) · Current Topics in Microbiology and Immunology · doi:10.1007/82_2012_303 · Cited by 366
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Metadata
- Authors
- Olier, M., Marcq, I., Salvador-Cartier, C., Secher, T., Dobrindt, U., Boury, M., Bacquié, V., Penary, M., Gaultier, E., Nougayrède, J.-P., Fioramonti, J., & Oswald, E.
- Venue
- Gut Microbes
- Publisher
- Informa UK Limited
- Published
- 2012-11-16
- Volume
- 3
- Issue
- 6
- Pages
- 501-509
- ISSN
- 1949-0976 (ISSN-L)
- 1949-0984
- OpenAlex
- W2005271513
- Cited by
- 159
- References
- 9
- FWCI
- 12.72
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Olier, M., Marcq, I., Salvador-Cartier, C., Secher, T., Dobrindt, U., Boury, M., Bacquié, V., Penary, M., Gaultier, E., Nougayrède, J.-P., Fioramonti, J., & Oswald, E. (2012). Genotoxicity of Escherichia coli Nissle 1917 strain cannot be dissociated from its probiotic activity. Gut Microbes. https://doi.org/10.4161/gmic.21737
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