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Cited by 15Barrier Structure and Function StudiesDermatology and Skin DiseasesAdvancements in Transdermal Drug Delivery

Up-regulation of tight junction-related proteins and increase of human epidermal keratinocytes barrier function by Saccharomycosis ferment filtrate

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Pang, Jong-Hwei, Wong, Wen-Rou, Hakozaki, Tomohiro, Yoshii, Takashi, Chen, Tzu-Ya·2011·Journal of Cosmetics, Dermatological Sciences and Applications
DOI 10.4236/jcdsa.2011.11003
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Abstract

Saccharomycopsis ferment filtrate (SFF), mainly used in skin care products, has been reported to inhibit inflammatory nitric oxide production and prevent epidermal damage. However, the effects of SFF on epidermal keratinocytes have not yet been explored. We investigated the effects of SFF on skin barrier function using human primary epidermal keratinocytes. Cell viability was determined by MTT assay. The mRNA and protein expression levels of tight junction proteins (claudin-1, -3, -4, occludin, ZO-1) were analyzed by RT-PCR and Western blotting, respectively. The effect of SFF on the barrier formation of epidermal keratinocytes was measured by transepithelial electrical resistance (TER). Rescue of cell death from H2O2 treatment was evaluated by annexin V staining. SFF, at concentrations that did not cause significant change of cell viability, induced dose-dependent cell-cell adhesion and formation of an organized monolayer structure. Pretreatment of keratinocytes with EGTA, a Ca2+ chelator, did not inhibit the cell-cell adhesion of keratinocytes by SFF, indicating a Ca2+-independent mechanism. The mRNA and protein levels of claudin-1 in keratinocytes were up-regulated by SFF treatment in a dose-dependent manner. The expressions of other tight junctions (TJs) including claudin-3 & 4, occludin and ZO-1 were also similarly increased in SFF-treated epidermal keratinocytes. The promoting effect of SFF on the barrier function of epidermal keratinocytes was further confirmed by the increased TER value in SFF-treated epidermal keratinocytes. Annexin V staining confirmed that SFF markedly decreased the number of dead cells resulted from H2O2 injury. Taken together, our results provided the first evidence that SFF enhanced keratinocytes barrier function by increasing the expression of TJs and TER.

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Research works printed but not linked: 38

  1. N. Kitson and J. L. Thewalt, "Hypothesis: The Epidermal Permeability Barrier is A Porous Medium," Acta Der- mato-Venereologica Supplementum, Vol. 208, 2000, pp: 12-15. doi:10.1080/000155500750042808 by Saccharomycosis Ferment Filtrate · DOI
  2. P. W. Wertz, "Lipids and Barrier Function of the Skin," Acta Dermato-Venereologica Supplementum, Vol. 208, 2000, pp. 7-11. doi:10.1080/000155500750042790 · DOI
  3. M. Furuse, M. Hata, K. Furuse, Y. Yoshida, A. Haratake and Y. Sugitani, et al, "Claudin-Based Tight Junctions Are Crucial for The Mammalian Epidermal Barrier: A Lesson From Claudin-1-Deficient Mice, " Journal of Cell Biology, Vol. 156, No. 6, 2002, pp. 1099-1111. doi:10.1083/jcb.200110122 · DOI · Paper page
  4. M. Malminen, V. Koivukangas, J. Peltonen, S. L. Kar- vonen, A. Oikarinen and S. Peltonen, "Immunohistologi- cal Distribution of The Tight Junction Components ZO-1 and Occludin In Regenerating Human Epidermis," British Journal of Dermatology, Vol. 149, No. 2, 2003, pp. 255-260. doi:10.1046/j.1365-2133.2003.05438.x · DOI
  5. K. Pummi, M. Malminen, H. Aho, S. L. Karvonen, J. Peltonen and S. Peltonen, "Epidermal Tight Junctions: Zo-1 and Occludin Are Expressed In Mature, Developing and Affected Skin and In Vitro Differentiating Keratino- cytes," Journal of Investigative Dermatology, Vol. 117, No. 5, 2001, pp. 1050-1058. doi:10.1046/j.0022-202x.2001.01493.x · DOI · Paper page
  6. K. Morita and Y. Miyachi, "Tight Junctions in the Skin," Journal of Dermatological Science, Vol. 31, No. 2, 2003, pp. 81-89. doi:10.1016/S0923-1811(03)00038-0 · DOI
  7. T. Yuki, A. Haratake, H. Koishikawa, K. Morita, Y. Mi- yachi and S. Inoue, "Tight Junction Proteins in Keratino- cytes: Localization and Contribution to Barrier Function," Expiermental Dermatology, Vol. 16, 2007, pp. 324-330. doi:10.1111/j.1600-0625.2006.00539.x · DOI
  8. J. M. Brandner, S. Kief, E. Wladykowski, P. Houdek and I. Moll, "Tight Junction Proteins In The Skin," Skin Pharmacology & Physiology, Vol. 1919, No. 2, pp. 71-77.
  9. D. Tsuruta, K. J. Green, S. Getsios and J. C. Jones, "The Barrier Function of Skin: How to Keep A Tight Lid On Water Loss," Trends in Cell Biology, Vol. 12, No. 8, 2002, pp. 355-357. doi:10.1016/S0962-8924(02)02316-4 · DOI
  10. G. Bazzoni and E. Dejana, "Keratinocyte Junctions and The Epidermal Barrier: How to Make A Skin-Tight Dress," Journal of Cell Biology, Vol. 156, No. 6, 2002, pp. 947-949. doi:10.1083/jcb.200202116 · DOI
  11. T. Yuki, A. Haratake, H. Koishikawa, K. Morita, Y. Mi- yachi and S. Inoue, "Tight Junction Proteins In Keratino- cytes: Localization and Contribution to Barrier Function," Expiermental Dermatology, Vol. 16, 2007, pp. 324-330. doi:10.1111/j.1600-0625.2006.00539.x · DOI
  12. J. P. Bentley, T. K. Hunt, J. B. Weiss, C. M. Taylor, A. N. Hanson, G. H. Davies, et al, "Peptides from Live Yeast Cell Derivative Stimulate Wound Healing," Archives of Surgery, Vol. 125, No. 5, 1990, pp. 641-646.
  13. J. Z. Kaplan, "Acceleration of Wound Healing by A Live Yeast Cell Derivative," Archives of Surgery, Vol. 119, No. 9, 1984, pp. 1005-1008.
  14. A. J. Dart, B. A. Dowling, C. L. Smith, "Topical treat- ments in equine wound management," Equine Practice, Vol. 21, No. 1, 2005, pp. 77-89. doi:10.1016/j.cveq.2004.11.003 · DOI
  15. J. M. Liptak, "An Overview of the Topical Management of Wounds," Australian Veterinary Journal, Vol. 75, No. 6, 1997, pp. 408-413. doi:10.1111/j.1751-0813.1997.tb14342.x · DOI
  16. M. J. Crowe, R. B. McNeill, D. J. Schlemm, D. G. Green- halgh and S. J. Keller, "Topical Application of Yeast Ex- tract Accelerates The Wound Healing Of Diabetic Mice," Journal of Burn Care & Rehabilitation, Vol. 2, 1920, pp. 155-162.
  17. H. H. Tsai, Y. C. Chen, W. R. Lee, C. H. Hu, T. Hako- zaki and T. Yoshii, et al, "Inhibition of Inflammatory Ni- tric Oxide Production and Epidermis Damages by Sac- charomycopsis Ferment Filtrate," Journal of Derma- tological Science, Vol. 42, No. 3, 2006, pp. 249-257. doi:10.1016/j.jdermsci.2006.01.009 · DOI
  18. K. Ikeyama, S. Fuziwara and M. Denda, "Topical Appli- cation Of Neuronal Nitric Oxide Synthase Inhibitor Ac- celerates Cutaneous Barrier Recovery and Prevents Epi- dermal Hyperplasia Induced By Barrier Disruption," Journal of Investigative Dermatology, Vol. 127, No. 7, 2007, pp. 1713-1719.
  19. S. Basuroy, A. Seth, B. Elias, A. P. Naren and R. Rao, "MAPK Interacts With Occludin and Mediates Egf-Induced Prevention of Tight Junction Disruption by Hydrogen Per- oxide," Biochemical Journal, Vol. 393, No. 1, 2006, pp. 69-77. doi:10.1042/BJ20050959 · DOI
  20. H. S. Lee, K. Namkoong, D. H. Kim, K. J. Kim, Y. H. Cheong and S. S. Kim, et al, "Hydrogen Peroxide-Induced Alterations of Tight Junction Proteins In Bovine Brain Microvascular Endothelial Cells," Microvascular Re- search, Vol. 68, No. 3, 2004, pp. 231-238. doi:10.1016/j.mvr.2004.07.005 · DOI
  21. J. C. Jones and R. D. Goldman, "Intermediate Filaments and The Initiation of Desmosome Assembly," Journal of Cell Biology, Vol. 101, No. 2, 1985, pp. 506-517. doi:10.1083/jcb.101.2.506 · DOI
  22. E. J. O'Keefe, R. A. Briggaman and B. Herman, "Cal- cium-Induced Assembly of Adherens Junctions in Kerati- nocytes," Journal of Cell Biology, Vol. 105, No. 2, 1987, pp. 807-817. doi:10.1083/jcb.105.2.807 · DOI
  23. H. Hennings and K. A. Holbrook, "Calcium Regulation of Cell-Cell Contact and Differentiation of Epidermal Cells in Culture. An ultrastructural study," Experimental Cell Research, Vol. 143, No. 1, 1983, pp. 127-142. doi:10.1016/0014-4827(83)90115-5 · DOI
  24. M. D. Kubler, P. W. Jordan, C. H. O'Neill and F. M. Watt, "Changes In The Abundance and Distribution of Actin and Associated Proteins During Terminal Differentiation of Human Epidermal Keratinocytes," Journal of Cell Sci- ence 100, 1991, pp. 153-65.
  25. J. E. Lewis, P. J. Jensen and M. J. Wheelock, "Cadherin Function Is Required for Human Keratinocytes to As- semble Desmosomes and Stratify In Response to Cal- cium," Journal of Investigative Dermatology, Vol. 102, No. 6, 1994, pp. 870-877. doi:10.1111/1523-1747.ep12382690 · DOI
  26. K. Pummi, M. Malminen, H. Aho, S. L. Karvonen, J. Peltonen and S. Peltonen, "Epidermal Tight Junctions: Zo-1 and Occludin Are Expressed In Mature, Developing and Affected Skin and In Vitro Differentiating Keratino- cytes," Journal of Investigative Dermatology, Vol. 117, No. 5, 2001, pp. 1050-1058. doi:10.1046/j.0022-202x.2001.01493.x · DOI · Paper page
  27. S. A. Francis, J. M. Kelly, J. McCormack, R. A. Rogers, J. Lai and E. E. Schneeberger, et al, "Rapid Reduction of MDCK Cell Cholesterol by Methyl-Beta-Cyclodextrin Alters Steady State Transepithelial Electrical Resistance," European Journal of Cell Biology, Vol. 78, No. 7, 1999, pp. 473-484.
  28. D. Roop, "Defects in the Barrier," Science, Vol. 267, No. 5197, 1995, pp. 474-475. doi:10.1126/science.7529942 · DOI
  29. T. Yuki, A. Haratake, H. Koishikawa, K. Morita and Y. Miyachi, "Tight Junction Proteins In Keratinocytes: Lo- calization and Contribution to Barrier Function," Experi- mental Dermatology, Vol. 16, No. 4, 2007, pp. 324-330. doi:10.1111/j.1600-0625.2006.00539.x · DOI
  30. F. M. Watt, "Stem cell fate and patterning in mammalian epidermis," Current Opinion in Genetics & Development, Vol. 11, No. 4, 2001, pp. 410-477. doi:10.1016/S0959-437X(00)00211-2 · DOI
  31. S. Getsios, A. C. Huen and K. J. Green, "Working Out The Strength And Flexibility of Desmosomes," Nature Reviews Molecular Cell Biology, Vol. 5, No. 4, pp: 271-281. doi:10.1038/nrm1356 · DOI · Paper page
  32. K. Matter, S. Aijaz, A. Tsapara and M. S. Balda, "Mam- malian Tight Junctions in the Regulation of Epithelial Differentiation and Proliferation," Current Opinion in Cell Biology, Vol. 17, No. 5, 2005, pp. 453-458. doi:10.1016/j.ceb.2005.08.003 · DOI
  33. F. Kramer, K. White and M. Kubbies, et al, "Genomic Organization of Claudin-1 and Its Assessment in Heredi- tary and Sporadic Breast Cancer," Human Genetics, Vol. 107, 2007, pp. 249-256.
  34. K. Swisshelm, A. Machl, S. Planitzer, R. Robertson, M. Kubbies and S. Hosier, "SEMP1, A Senescence-Associated cDNA Isolated From Human Mammary Epithelial Cells, Is A Member of An Epithelial Membrane Protein Super- family," Gene, Vol. 226, No. 2, 1999, pp. 285-295. doi:10.1016/S0378-1119(98)00553-8 · DOI
  35. K. Morita, S. Tsukita and Y. Miyachi, "Tight Junction- Associated Proteins (Occludin, Zo-1, Claudin-1, Claudin-4) In Squamous Cell Carcinoma and Bowen's Disease," Brit- ish Journal of Dermatolog, Vol. 151, No. 2, 2004, pp. 328-334. doi:10.1111/j.1365-2133.2004.06029.x · DOI
  36. K. V. Roskos and R. H. Guy, "Assessment of Skin Barrier Function Using Transepidermal Water Loss: Effect of Age," Pharmaceutical Research, Vol. 6, No. 11, 1989, pp: 949-953. doi:10.1023/A:1015941412620 · DOI
  37. K. P. Wilhelm, A. B. Cua and H. I. Maibach, "Skin aging effect on Transepidermal Water Loss, Stratum Corneum Hydration, Skin Surface Ph and Casual Sebum Content," Archives of Dermatology, Vol. 127, No. 12, 1991, pp. 1806-1809. doi:10.1001/archderm.127.12.1806 · DOI
  38. D. C. Barnard, K. Ryan, J. L. Manley and J. D. Richter, "Symplekin and xGLD-2 are required for Cpeb-Mediated Cytoplasmic Polyadenylation," Cell, Vol. 119, No. 5, 2004, pp. 641-651. doi:10.1016/j.cell.2004.10.029 · DOI

Cited by

Sorted by citations

Authors
Pang, J.-H., Wong, W.-R., Hakozaki, T., Yoshii, T., & Chen, T.-Y.
Affiliations
  • Chang Gung University (TW)
  • Procter & Gamble (United States) (US)
ORCID
Venue
Journal of Cosmetics, Dermatological Sciences and Applications
Publisher
Scientific Research Publishing, Inc.
Published
2011
Volume
01
Issue
01
Pages
15-24
ISSN
  • 2161-4105 (ISSN-L)
  • 2161-4512
DOI
10.4236/jcdsa.2011.11003
OpenAlex
W2042523803
Cited by
15
References
1
Citations by year
2024: 4 · 2023: 4 · 2022: 2 · 2019: 1 · 2017: 1 · 2015: 1 · 2014: 1 · 2012: 1
FWCI
0.35
Type
journal article
Language
en
Open access
Licence
Locations
Journal of Cosmetics Dermatological Sciences and Applications (publishedVersion, cc-by)
Topics
  • Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
  • Health Sciences › Medicine › Dermatology › Dermatology and Skin Diseases
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advancements in Transdermal Drug Delivery
Keywords
epidermal keratinocytes, skin barrier function, tight junction proteins, claudin-1, claudin-3, claudin-4, occludin, ZO-1, transepithelial electrical resistance, cell-cell adhesion, hydrogen peroxide-induced cell death, skin care products

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