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Cited by 108Pluripotent Stem Cells ResearchCongenital heart defects researchCardiac electrophysiology and arrhythmias

Current methods for the maturation of induced pluripotent stem cell-derived cardiomyocytes

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Machiraju, Pranav, Greenway, Steven C·2019·World Journal of Stem Cells
DOI 10.4252/wjsc.v11.i1.33PMC PMC6354100
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Abstract

modelling. Cardiovascular disease is the greatest cause of mortality worldwide but encompasses rarer disorders of conduction and myocardial function for which a cellular model of study is ideal. Although methods to differentiate iPSCs into beating cardiomyocytes (iPSC-CMs) have recently been adequately optimized and commercialized, the resulting cells remain largely immature with regards to their structure and function, demonstrating fetal gene expression, disorganized morphology, reliance on predominantly glycolytic metabolism and contractile characteristics that differ from those of adult cardiomyocytes. As such, disease modelling using iPSC-CMs may be inaccurate and of limited utility. However, this limitation is widely recognized, and numerous groups have made substantial progress in addressing this problem. This review highlights successful methods that have been developed for the maturation of human iPSC-CMs using small molecules, environmental manipulation and 3-dimensional (3D) growth approaches.

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Cited by

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Authors
Machiraju, P., & Greenway, S. C.
Affiliations
University of Calgary (CA)
ORCID
Venue
World Journal of Stem Cells
Publisher
Baishideng Publishing Group Inc.
Published
2019-01-26
Volume
11
Issue
1
Pages
33-43
ISSN
1948-0210 (ISSN-L)
DOI
10.4252/wjsc.v11.i1.33
PMC
PMC6354100
OpenAlex
W2911855595
Cited by
108
References
8
Citations by year
2026: 3 · 2025: 9 · 2024: 6 · 2023: 12 · 2022: 6 · 2021: 32 · 2020: 27 · 2019: 13
FWCI
1.72
Type
journal article
Language
en
Open access
Licence
cc-by-nc
Locations
  • World Journal of Stem Cells (publishedVersion, cc-by-nc)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Congenital heart defects research
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac electrophysiology and arrhythmias
Keywords
induced pluripotent stem cells, iPSC-derived cardiomyocytes, cardiomyocyte maturation, beating cardiomyocytes, disease modeling, small molecules, three-dimensional growth, cardiomyocyte contractility, glycolytic metabolism, fetal gene expression, cardiovascular disease

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