A Global Approach to Asses Stress Response of the Bioregenerative Life Support System Organism Rhodospirillum rubrum Under Space-Flight Related Environmental Conditions
Abstract
In view of long haul space exploration missions, the development of regenerative life support systems is a condition sine qua non to increase the crew autonomy and decrease the cost associated to the mass embarked. Therefore, in the late 80's, the European Space Agency initiated the MELiSSA project (Micro-Ecological Life Support System Alternative). MELISSA has been conceived as a micro-organisms and higher plant process enabling high recycling efficiency. Nevertheless, the organisms inhabiting the the MELISSA system need to perform their tasks as optimally as possible (Fig. 1). A number of stresses such as temperature variation, water deprivation, oxidative stress, UV and ionizing radiations, light variation, supernatant of the previous compartment, genomic evolution during long-time culturing and gene transfer can indeed affect the capabilities of the micro-organisms as well as the efficiency of the whole bioregenerative loop. Thus, the detection of functional and genetic instability due to stress in all bioreactors of the MELiSSA loop is considered to be of primordial importance. In this paper a global approach is put forward to analyse the stress response of R. rubrum ATCC25903, using whole cell flow cytometry analysis, mass spectrometry analysis and microarray gene expression profiling.
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The effects observed in microgravity vary with the growth medium and growth conditions used.
“Several effects have been observed in microgravity (Hendrickx et al., 2005, Mastroleo et al., 2008, 2009), but the responses are depending on the growth media and growth conditions used.”
From Limnospira indica PCC8005 growth in photobioreactor: model and simulation of the ISS and ground experiments (2020) · cites this paper as Hendrickx et al., 2005
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- Authors
- Hendrickx, L., Mastroleo, F., Baatout, S., Mergeay, M., Paillé, C., & Wattiez, R.
- Affiliations
- Belgian Nuclear Research Centre (BE)
- Venue
- SAE Technical Paper Series
- Publisher
- SAE International
- Published
- 2005-07-11
- Volume
- 1
- ISSN
- 0148-7191 (ISSN-L)
- 2688-3627
- OpenAlex
- W2041883126
- Cited by
- 1
- References
- 23
- Citations by year
- 2017: 1
- FWCI
- 0.00
- Type
- conference paper
- Language
- en
- Open access
- closed
- Locations
- SAE technical papers on CD-ROM/SAE technical paper series (publishedVersion)
- Funders
- European Space Agency
- Fonds De La Recherche Scientifique - FNRS
- Topics
- Health Sciences › Medicine › Physiology › Spaceflight effects on biology
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Physical Sciences › Engineering › Aerospace Engineering › Space Exploration and Technology
- Keywords
- Rhodospirillum rubrum, bioregenerative life support, Melissa, stress response, mass spectrometry, gene expression microarrays, microbial stress tolerance, long-duration spaceflight
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