The DOI matches its Crossref record.
Direct counts of aquatic bacteria by a modified epifluorescence technique1
Abstract
Various excitation and emission filters, microscope lamps, membrane filters, and field storage techniques used for direct counting of bacteria by epifluorescence microscopy have been compared. A rapid, simple modification of the Acridine Orange procedure using a new light filter combination, Sartorius cellulose membrane filters, and distilled water rinse gave the brightest cell fluorescence, darkest background, least fading, and highest counts for both estuarine and freshwater samples. Field fixation with Formalin permits storage of bacterial samples for 1 to 2 weeks without changes in bacterial numbers.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Use of nuclepore filters for counting bacteria by fluorescence microscopy
Hobbie, J. E., Daley, R. J., & Jasper, S. (1977) · Applied and Environmental Microbiology · doi:10.1128/aem.33.5.1225-1228.1977 · Cited by 4,244
Self-citationFuhrman, J. A., & Azam, F. (1980) · Applied and Environmental Microbiology · doi:10.1128/aem.39.6.1085-1095.1980 · Cited by 873
Determination of bacterial number and biomass in the marine environment
Watson, S. W., Novitsky, T. J., Quinby, H. L., & Valois, F. W. (1977) · Applied and Environmental Microbiology · doi:10.1128/aem.33.4.940-946.1977 · Cited by 590
Lampitt, R. S., Wishner, K. F., Turley, C. M., & Angel, M. V. (1993) · Marine Biology · doi:10.1007/bf00355486 · Cited by 317
Barbosa, A. B., Mosley, B. A., Galvão, H. M., & Domingues, R. B. (2023) · Microorganisms · doi:10.3390/microorganisms11102559 · Cited by 2
Metadata
- Authors
- Daley, R. J., & Hobbie, J. E.
- Venue
- Limnology and Oceanography
- Publisher
- Wiley
- Published
- 1975-09
- Volume
- 20
- Issue
- 5
- Pages
- 875-882
- ISSN
- 0024-3590 (ISSN-L)
- 1939-5590
- OpenAlex
- W2070790580
- Cited by
- 326
- References
- 0
- FWCI
- 12.53
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Direct counts of aquatic bacteria by a modified epifluorescence technique1. https://app.cochat.ai/papers/10.4319/lo.1975.20.5.0875
- The paper
- Daley, R. J., & Hobbie, J. E. (1975). Direct counts of aquatic bacteria by a modified epifluorescence technique1. Limnology and Oceanography. https://doi.org/10.4319/lo.1975.20.5.0875
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
