Antifungal Susceptibility Testing with Etest for Candida Species Isolated from Patients with Oral Candidiasis
Open accessAbstract
BACKGROUND: The necessity of performing antifungal susceptibility tests is recently increasing because of frequent cases of oral candidiasis caused by antifungal-resistant Candida species. The Etest (BioMerieux, Marcy l'Etoile, France) is a rapid and easy-to-perform in vitro antifungal susceptibility test. OBJECTIVE: The purpose of this study was to determine the minimal inhibitory concentrations (MICs) of antifungal agents by using the Etest for Candida species isolated from patients with oral candidiasis. METHODS: Forty-seven clinical isolates of Candida species (39 isolates of Candida albicans, 5 isolates of C. glabrata, and 3 isolates of C. tropicalis) were tested along with a reference strain (C. albicans ATCC 90028). The MIC end points of the Etest for fluconazole, itraconazole, voriconazole, and amphotericin B susceptibility were read after the 24-hour incubation of each isolate on RPMI 1640 agar. RESULTS: All Candida isolates were found susceptible to voriconazole and amphotericin B. However, all five isolates of C. glabrata were resistant to itraconazole, among which two isolates were also resistant to fluconazole. CONCLUSION: This study revealed that the Etest represented a simple and efficacious method for antifungal susceptibility testing of Candida species isolated from oral candidiasis patients. Therefore, voriconazole and amphotericin B should be recommended as effective alternatives for the treatment of oral candidiasis.
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Song et al. reported from South Korea that two of five C. glabrata isolates tested were resistant to fluconazole.
“Song et al. [95] reported from South Korea that two of the five C. glabrata isolates tested were resistant to fluconazole.”
From Candida glabrata: pathogenicity and resistance mechanisms for adaptation and survival (2021) · cites this paper as [95]
The five C. glabrata isolates in Song et al.'s study were resistant to itraconazole.
“The five isolates were resistant to itraconazole.”
From Candida glabrata: pathogenicity and resistance mechanisms for adaptation and survival (2021) · cites this paper as [95]
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- Authors
- Song, Y. B., Suh, M. K., Ha, G. Y., & Kim, H.
- Affiliations
- Dongguk University (KR)
- Corresponding author
- Suh, Moo Kyu
- Venue
- Annals of Dermatology
- Publisher
- XMLink
- Published
- 2015
- Volume
- 27
- Issue
- 6
- Pages
- 715
- ISSN
- 1013-9087 (ISSN-L)
- 2005-3894
- PMC
- PMC4695424
- OpenAlex
- W2284331280
- Cited by
- 35
- References
- 31
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 6 · 2023: 3 · 2022: 3 · 2021: 3 · 2020: 5 · 2019: 2 · 2018: 4 · 2017: 1 · 2016: 2
- FWCI
- 1.82
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Annals of Dermatology (publishedVersion, cc-by-nc)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Yeasts and Rust Fungi Studies
- Keywords
- antifungal susceptibility testing, E-test, oral candidiasis, Candida albicans, Candida glabrata, Candida tropicalis, minimum inhibitory concentration, fluconazole resistance, itraconazole resistance, voriconazole, amphotericin B, antifungal resistance
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