Candida glabrata and Candida albicans co‐infection of an in vitro oral epithelium
Open accessAbstract
BACKGROUND: Candida albicans is regarded as the leading of candidosis. However, Candida glabrata has emerged as an important pathogen of oral mucosa, occurring both singly or in mixed species infections, often with C. albicans. Compared with C. albicans, little is known about the role of C. glabrata in oral infection. The aim of this study was to examine single and mixed species infection of oral epithelium involving C. glabrata and establish its ability to invade and damage tissue. METHODS: A reconstituted human oral epithelium (RHOE) was infected only with C. glabrata, or simultaneously with C. glabrata and C. albicans. The ability of both species to invade the tissue was examined using species specific peptide nucleic acid (PNA) probe hybridization and confocal laser scanning microscopy. Epithelial damage was assessed by measuring lactate dehydrogenase (LDH) activity. RESULTS: Candida glabrata strains were able to colonize the RHOE, in a strain dependent manner. Candida glabrata single infection after 12 h, generally revealed no invasion of the RHOE, which contrasted with extensive tissue invasion demonstrated by C. albicans. Mixed infection showed that C. albicans enhanced the invasiveness of C. glabrata, and led to increased LDH release by the RHOE, which paralleled the observed histological damage. CONCLUSIONS: The results obtained demonstrating enhanced invasion and increased tissue damage caused by mixed C. glabrata and C. albicans infections have important clinical significance and highlight the need to identify Candida species involved in oral candidosis.
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C. glabrata could not invade the reconstituted human oral epithelium in vitro.
“Similarly, C. glabrata could neither degrade the E-Cadherin protein present in the adherens junctions of the oral mucosal epithelium [135] nor invade the reconstituted human oral epithelium in vitro [130].”
From Candida glabrata: A Lot More Than Meets the Eye (2019) · cites this paper as [130]
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- Authors
- Silva, S., Henriques, M., Hayes, A., Oliveira, R., Azeredo, J., & Williams, D. W.
- Affiliations
- Institute for Biotechnology and Bioengineering (PT)
- Cardiff University (GB)
- Corresponding author
- Henriques, Mariana
- Venue
- Journal of Oral Pathology & Medicine
- Publisher
- Wiley
- Published
- 2010-12-15
- Volume
- 40
- Issue
- 5
- Pages
- 421-427
- ISSN
- 0300-9777 (ISSN-L)
- 0904-2512
- 1600-0714
- OpenAlex
- W1593066164
- Cited by
- 108
- References
- 31
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 2 · 2023: 8 · 2022: 14 · 2021: 8 · 2020: 10 · 2019: 9 · 2018: 7 · 2017: 10 · 2016: 6 · 2015: 8 · 2014: 8 · 2013: 9 · 2012: 2
- FWCI
- 2.27
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Oral Pathology and Medicine (publishedVersion)
- PubMed (publishedVersion)
- Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, other-oa)
- Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, cc-by-nc-nd)
- Funders
- Fundação para a Ciência e a Tecnologia
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Yeasts and Rust Fungi Studies
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › HIV/AIDS oral health manifestations
- Keywords
- Candida glabrata, Candida albicans, oral candidiasis, mixed fungal infection, oral epithelium, tissue invasion, epithelial damage, lactate dehydrogenase release
- MeSH terms
- Analysis of Variance, Candida albicans, Candidiasis, Oral, Epithelium, Humans, Mouth Mucosa, Superinfection, Keratinocytes, Microscopy, Confocal, Statistics, Nonparametric, Candida glabrata, Lactate Dehydrogenases, Cell Line, Tumor, Tissue Culture Techniques
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