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Fluorescent biological aerosol particles measured with the Waveband Integrated Bioaerosol Sensor WIBS-4: laboratory tests combined with a one year field study
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Abstract
In this paper bioaerosol measurements conducted with the Waveband Integrated Bioaerosol Sensor mark 4 (WIBS-4) are presented. The measurements comprise aerosol chamber characterization experiments and a one-year ambient measurement period at a semi-rural site in South Western Germany. This study aims to investigate the sensitivity of WIBS-4 to biological and non-biological aerosols and detection of biological particles in the ambient aerosol. Several types of biological and non-biological aerosol samples, including fungal spores, bacteria, mineral dust, ammonium sulphate, combustion soot, and fluorescent polystyrene spheres, were analyzed by WIBS-4 in the laboratory. The results confirm the sensitivity of the ultraviolet light-induced fluorescence (UV-LIF) method to biological fluorophores and show the good discrimination capabilities of the two excitation wavelengths/detection wavebands method applied in WIBS-4. However, a weak cross-sensitivity to non-biological fluorescent interferers remains and is discussed in this paper. All the laboratory studies have been undertaken in order to prepare WIBS-4 for ambient aerosol measurements. According to the one-year ambient aerosol study, number concentration of fluorescent biological aerosol particles (FBAP) show strong seasonal and diurnal variability. The highest number concentration of FBAP was measured during the summer term and decreased towards the winter period when colder and drier conditions prevail. Diurnal FBAP concentra…
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Long-term analysis of FBAP measurements, including periods at Karlsruhe and Hyytiälä, shows an annual cycle of average FBAP number concentrations peaking in summer and lowest in winter.
“Long-term analysis of FBAP measurements, including periods at the Karlsruhe (Toprak and Schnaiter, 2013) and Hyytiälä site (Schumacher et al., 2013) discussed here, shows an annual cycle of average FBAP number concentrations peaking in summer and lowest in winter.”
From Regional-scale simulations of fungal spore aerosols using an emission parameterization adapted to local measurements of fluorescent biological aerosol particles (2015) · cites this paper as Toprak and Schnaiter, 2013
A better correlation with measured FBAP concentrations is achieved for specific humidity rather than relative humidity.
“Analyzing the meteorological and surface parameters of the model output, it was found that a better correlation with the measured FBAP concentrations is achieved for specific humidity rather than relative humidity, as it was reported for previous field measurements (Gabey et al., 2010; Toprak and Schnaiter, 2013; Di Filippo et al., 2013).”
From Regional-scale simulations of fungal spore aerosols using an emission parameterization adapted to local measurements of fluorescent biological aerosol particles (2015) · cites this paper as Toprak and Schnaiter, 2013
Measured FBAP number concentrations often exhibit distinct diel (24 h) cycles with a maximum in the morning hours or around midnight and a minimum around noon.
“Measured FBAP number concentrations often exhibit distinct diel (24 h) cycles with a maximum in the morning hours or around midnight and a minimum around noon. These features have been consistently reported by most studies discussing temporal behavior of FBAP (Gabey et al., 2010; Huffman et al., 2010, 2012; Toprak and Schnaiter, 2013).”
From Regional-scale simulations of fungal spore aerosols using an emission parameterization adapted to local measurements of fluorescent biological aerosol particles (2015) · cites this paper as Toprak and Schnaiter, 2013
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Hummel, M., Hoose, C., Gallagher, M., Healy, D. A., Huffman, J. A., O'Connor, D., Pöschl, U., Pöhlker, C., Robinson, N. H., Schnaiter, M., et al. (2015) · Atmospheric Chemistry and Physics · doi:10.5194/acp-15-6127-2015 · Cited by 59
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Metadata
- Authors
- Toprak, E., & Schnaiter, M.
- Affiliations
- Karlsruhe Institute of Technology (DE)
- Venue
- Atmospheric Chemistry and Physics
- Publisher
- Copernicus GmbH
- Published
- 2013-01-10
- Volume
- 13
- Issue
- 1
- Pages
- 225-243
- ISSN
- 1680-7316 (ISSN-L)
- 1680-7324
- OpenAlex
- W2044059536
- Cited by
- 171
- References
- 1
- Citations by year
- 2026: 12 · 2025: 15 · 2024: 8 · 2023: 11 · 2022: 13 · 2021: 13 · 2020: 11 · 2019: 15 · 2018: 12 · 2017: 15 · 2016: 16 · 2015: 13 · 2014: 9 · 2013: 7
- FWCI
- 10.98
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Atmospheric chemistry and physics (publishedVersion, cc-by)
- Repository KITopen (Karlsruhe Institute of Technology) (publishedVersion)
- Repository KITopen (Karlsruhe Institute of Technology) (publishedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- KITopen (other-oa)
- KITopen (cc-by)
- Greater South Information System
- Funders
- University of Hertfordshire
- Deutsche Forschungsgemeinschaft
- Topics
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Indoor Air Quality and Microbial Exposure
- Physical Sciences › Earth and Planetary Sciences › Atmospheric Science › Atmospheric chemistry and aerosols
- Physical Sciences › Environmental Science › Global and Planetary Change › Atmospheric aerosols and clouds
- Keywords
- bioaerosols, fungal spores, bacterial aerosols, seasonal and diurnal variability, relative humidity, aerosol number concentration
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