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Fluorescent biological aerosol particles measured with the Waveband Integrated Bioaerosol Sensor WIBS-4: laboratory tests combined with a one year field study

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Toprak, E., Schnaiter, M.·2013·Atmospheric Chemistry and Physics

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Abstract

In this paper bioaerosol measurements conducted with the Waveband Integrated Bioaerosol Sensor mark 4 (WIBS-4) are presented. The measurements comprise aerosol chamber characterization experiments and a one-year ambient measurement period at a semi-rural site in South Western Germany. This study aims to investigate the sensitivity of WIBS-4 to biological and non-biological aerosols and detection of biological particles in the ambient aerosol. Several types of biological and non-biological aerosol samples, including fungal spores, bacteria, mineral dust, ammonium sulphate, combustion soot, and fluorescent polystyrene spheres, were analyzed by WIBS-4 in the laboratory. The results confirm the sensitivity of the ultraviolet light-induced fluorescence (UV-LIF) method to biological fluorophores and show the good discrimination capabilities of the two excitation wavelengths/detection wavebands method applied in WIBS-4. However, a weak cross-sensitivity to non-biological fluorescent interferers remains and is discussed in this paper. All the laboratory studies have been undertaken in order to prepare WIBS-4 for ambient aerosol measurements. According to the one-year ambient aerosol study, number concentration of fluorescent biological aerosol particles (FBAP) show strong seasonal and diurnal variability. The highest number concentration of FBAP was measured during the summer term and decreased towards the winter period when colder and drier conditions prevail. Diurnal FBAP concentra…

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Authors
Toprak, E., & Schnaiter, M.
Affiliations
Karlsruhe Institute of Technology (DE)
Venue
Atmospheric Chemistry and Physics
Published
2013-01-10
Volume
13
Issue
1
Pages
225-243
ISSN
  • 1680-7316 (ISSN-L)
  • 1680-7324
OpenAlex
W2044059536
Cited by
171
References
1
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FWCI
10.98
Type
journal article
Language
en
Open access
Locations
  • Atmospheric chemistry and physics (publishedVersion, cc-by)
  • Repository KITopen (Karlsruhe Institute of Technology) (publishedVersion)
  • Repository KITopen (Karlsruhe Institute of Technology) (publishedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • KITopen (other-oa)
  • KITopen (cc-by)
  • Greater South Information System
Funders
  • University of Hertfordshire
  • Deutsche Forschungsgemeinschaft
Topics
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Indoor Air Quality and Microbial Exposure
  • Physical Sciences › Earth and Planetary Sciences › Atmospheric Science › Atmospheric chemistry and aerosols
  • Physical Sciences › Environmental Science › Global and Planetary Change › Atmospheric aerosols and clouds
Keywords
bioaerosols, fungal spores, bacterial aerosols, seasonal and diurnal variability, relative humidity, aerosol number concentration

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