Producing a Superior Genotype from Agria Potato Cultivar Using Somaclonal Variation
Open accessAbstract
This study was performed to produce a superior genotype from Agria potato cultivar using somaclonal variation. Two leaf and meristem explants in combination with four doses of 2,4-D (0, 2, 3 and 4 mg / l) were used for callus induction in a factorial model based on a completely randomized design with 3 replications. Results showed that the meristem explant, along with 3 mg 2,4-D produced the most suitable calluses. In the mentioned regeneration media, the best calluses were regenerated and one of the regenerated genotypes, which was very different from the parent cultivar was selected. The regenerated genotype, was compared with the maternal genotype (Agria) and a control cultivar (Sante), in a field experiment based on a randomized complete block design with 3 replications. The results showed that in terms of most of the studied traits such as tuber weight per plant, stolon length, percentage of dry matter and percentage of starch, the new genotype was superior, compared to the parent cultivar and in terms of peel percentage and maturity date, the parental cultivar was superior. The results of the molecular comparison also showed that based on CBDP marker, both in terms of band number and band size, there were differences between the new genotype and the parental cultivar. In general, results showed that somaclonal variation can be an effective method to generate new genotypes with superior characteristics.
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Reducing PGR concentrations can significantly decrease the likelihood of somaclonal variation.
“Studies have shown that reducing PGR concentrations can significantly decrease the like-lihood of somaclonal variation [68,162], while optimizing environmental conditions such as temperature and light intensity can further stabilize cultures by avoiding stress-induced changes [68].”
From Somaclonal Variation and Clonal Fidelity in Commercial Micropropagation: Challenges and Perspectives (2025) · cites this paper as [68]
The Introduction (page not specified) says the hormonal combination of a culture medium can cause genetic changes. The Results and Discussion (page not specified) find significant effects of 2,4-D concentration on callus induction, diameter and weight—not on the likelihood of somaclonal variation. The paper does not establish that lowering PGR concentrations significantly reduces that likelihood. (LLM, conf=0.78)
Experimental — accuracy not yet measuredThe induced variation can be screened for desired traits to create new cultivars with desirable quality, known as somaclonal selection.
“The induced variation can be screened for desired traits to create new cultivars with desirable quality, known as somaclonal selection [68].”
From Somaclonal Variation and Clonal Fidelity in Commercial Micropropagation: Challenges and Perspectives (2025) · cites this paper as [68]
Selecting genetically stable genotypes can significantly reduce somaclonal variation.
“Therefore, selecting genetically stable genotypes-those less prone to genetic changes during tissue culture-can significantly reduce somaclonal variation [68].”
From Somaclonal Variation and Clonal Fidelity in Commercial Micropropagation: Challenges and Perspectives (2025) · cites this paper as [68]
The Introduction (page not provided) discusses detecting somaclonal variation and factors associated with genetic changes, such as subcultures and culture-medium hormones. The Methods describe selecting genotypes for desirable growth traits, not for genetic stability. Searches for genetically stable genotypes and their selection found no passage saying that selecting them significantly reduces somaclonal variation. (LLM, conf=0.73)
Experimental — accuracy not yet measuredOptimizing environmental conditions such as temperature and light intensity can further stabilize cultures by avoiding stress-induced changes.
“while optimizing environmental conditions such as temperature and light intensity can further stabilize cultures by avoiding stress-induced changes [68].”
From Somaclonal Variation and Clonal Fidelity in Commercial Micropropagation: Challenges and Perspectives (2025) · cites this paper as [68]
Materials and Methods reports a light–dark schedule and growth-chamber temperatures, but does not test whether adjusting them stabilizes cultures. The Introduction links genetic changes to subcultures and culture-medium hormones; searches for light intensity, temperature optimization, and stress-induced changes found no passage connecting those conditions to culture stability. (LLM, conf=0.72)
Experimental — accuracy not yet measured
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- Authors
- Bayati, E., Gomarian, M., Mirzaie-Nodousha, H., Changizi, M., & Khaghani, S.
- Affiliations
- Islamic Azad University, Arak (IR)
- Research Institute of Forests and Rangelands (IR)
- ORCID
- Khaghani, Shahab
- Venue
- Nexo Revista Científica
- Publisher
- Consejo Nacional de Rectores
- Published
- 2021-06-07
- Volume
- 34
- Issue
- 02
- Pages
- 671-681
- ISSN
- 1818-6742 (ISSN-L)
- 1995-9516
- OpenAlex
- W3169493015
- Cited by
- 8
- References
- 17
- Citations by year
- 2025: 4 · 2024: 2 · 2023: 2
- FWCI
- 0.31
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Locations
- Nexo Revista Científica (publishedVersion, cc-by)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Genetic and Mutation Studies
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetics and Plant Breeding
- Keywords
- somaclonal variation, potato breeding, callus induction, 2,4-D concentration, meristem explants, leaf explants, plant regeneration, tuber weight, starch content, maturity date
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