Acceleration of heat shock-induced collagen breakdown in human dermal fibroblasts with knockdown of NF-E2-related factor 2
Open accessAbstract
Heat shock increases skin temperature during sun exposure and some evidence indicates that it may be involved in skin aging. The antioxidant response mediated by the transcription factor NF-E2-related factor 2 (Nrf2) is a critically important cellular defense mechanism that serves to limit skin aging. We investigated the effects of heat shock on collagenase expression when the antioxidant defense system was downregulated by knockdown of Nrf2. GSH and collagenases were analyzed, and the expression of inducible Nrf2, HO-1, and NQO1 was measured. HS68 cells were transfected with small interfering RNA against Nrf2. Heat shock induced the downregulation of Nrf2 in both the cytosol and nucleus and reduced the expression of HO-1, GSH, and NQO1. In addition, heat-exposed Nrf2-knockdown cells showed significantly increased levels of collagenase protein and decreased levels of procollagen. Our data suggest that Nrf2 plays an important role in protection against heat shock-induced collagen breakdown in skin.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Nrf2 deficiency or Nrf2-knockdown causes lipid oxidation, inflammation, and ECM-protease expression (e.g., MMPs, Cox) in UVA-irradiated skin fibroblasts or heat shock-induced human dermal fibroblasts.
“For example, Nrf2 deficiency or Nrf2-knockdown is known to cause lipid oxidation, inflammation, and extracellular matrix-protease expression (e.g., MMPs, cyclo-oxygenases (Cox) in UVA-irradiated skin fibroblasts or heat shock-induced human dermal fibroblasts [74,75].”
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
REST, M. V. D., & Garrone, R. (1991) · The FASEB Journal · doi:10.1096/fasebj.5.13.1916105 · Cited by 1,278 · see citing works
Nrf2 signaling: An adaptive response pathway for protection against environmental toxic insults
Osburn, W. O., & Kensler, T. W. (2007) · Mutation Research/Reviews in Mutation Research · doi:10.1016/j.mrrev.2007.11.006 · Cited by 549 · see citing works
Modulation of Skin Collagen Metabolism in Aged and Photoaged Human Skin In Vivo
Chung, J. H., Seo, J. Y., Choi, H. R., Lee, M. K., Youn, C. S., Rhie, G., Cho, K. H., Kim, K. H., Park, K. C., & Eun, H. C. (2001) · Journal of Investigative Dermatology · doi:10.1046/j.0022-202x.2001.01544.x · Cited by 410 · see citing works
Braun, S., Hanselmann, C., Gassmann, M. G., auf dem Keller, U., Born-Berclaz, C., Chan, K., Kan, Y. W., & Werner, S. (2002) · Molecular and Cellular Biology · doi:10.1128/mcb.22.15.5492-5505.2002 · Cited by 410 · see citing works
Chen, X., & KUNSCH, C. A. (2004) · Current Pharmaceutical Design · doi:10.2174/1381612043452901 · Cited by 387 · see citing works
Emerging Role of Nrf2 in Protecting Against Hepatic and Gastrointestinal Disease
Aleksunes, L. M., & Manautou, J. E. (2007) · Toxicologic Pathology · doi:10.1080/01926230701311344 · Cited by 285 · see citing works
Cutaneous effects of infrared radiation: from clinical observations to molecular response mechanisms
Schieke, S. M., Schröeder, P., & Krutmann, J. (2003) · Photodermatology Photoimmunology & Photomedicine · doi:10.1034/j.1600-0781.2003.00054.x · Cited by 271 · see citing works
The Missing Link – Light-Induced (280–1,600 nm) Free Radical Formation in Human Skin
Zastrow, L., Groth, N., Klein, F., Kockott, D., Lademann, J. M., Renneberg, R., & Ferrero, L. (2008) · Skin Pharmacology and Physiology · doi:10.1159/000188083 · Cited by 240 · see citing works
Hirota, A., Kawachi, Y., Itoh, K., Nakamura, Y., Xu, X., Banno, T., Takahashi, T., Yamamoto, M. P., & Otsuka, F. (2005) · Journal of Investigative Dermatology · doi:10.1111/j.0022-202x.2005.23670.x · Cited by 162 · see citing works
Shin, M. H., Moon, Y. J., Seo, J., Lee, Y., Kim, K. H., & Chung, J. H. (2007) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2007.10.053 · Cited by 147 · see citing works
Park, C., Lee, M. J., Ahn, J., Kim, S., Kim, H. H., Kim, K. H., Eun, H. C., & Chung, J. H. (2004) · Journal of Investigative Dermatology · doi:10.1111/j.0022-202x.2004.23487.x · Cited by 146 · see citing works
Şahin, K., Orhan, C., Tuzcu, Z., et al. (2012) · Food and Chemical Toxicology · doi:10.1016/j.fct.2012.08.029 · Cited by 139 · see citing works
Cho, S., Lee, M. J., Kim, M. S., Lee, S., Kim, Y. K., Lee, D. H., Lee, C. W., Cho, K. H., & Chung, J. H. (2008) · Journal of Dermatological Science · doi:10.1016/j.jdermsci.2007.11.009 · Cited by 134 · see citing works
Şahin, K., Orhan, C., Akdemir, F., Tuzcu, M., Iben, C., & Şahin, N. (2011) · Journal of Animal Physiology and Animal Nutrition · doi:10.1111/j.1439-0396.2010.01123.x · Cited by 111 · see citing works
Acceleration of UVB-induced photoageing in nrf2 gene-deficient mice
Hirota, A., Kawachi, Y., Yamamoto, M. P., Koga, T., Hamada, K., & Otsuka, F. (2011) · Experimental Dermatology · doi:10.1111/j.1600-0625.2011.01292.x · Cited by 88 · see citing works
The role of Nrf2 in ultraviolet A mediated heme oxygenase 1 induction in human skin fibroblasts
Zhong, J. L., Edwards, G. P., Raval, C., Li, H., & Tyrrell, R. M. (2010) · Photochemical & Photobiological Sciences · doi:10.1039/b9pp00068b · Cited by 65 · see citing works
Heat‐induced MMP‐1 expression is mediated by TRPV1 through PKC α signaling in HaCaT cells
Lee, Y. M., Li, W. H., Kim, Y. K., Kim, K. H., & Chung, J. H. (2008) · Experimental Dermatology · doi:10.1111/j.1600-0625.2008.00738.x · Cited by 53 · see citing works
Park, G., Jang, D. S., & Oh, M. S. (2012) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2012.04.013 · Cited by 41 · see citing works
Self-citationChronic heat treatment causes skin wrinkle formation and oxidative damage in hairless mice
Shin, M. H., Seo, J., Kim, Y. K., Kim, K. H., & Chung, J. H. (2012) · Mechanisms of Ageing and Development · doi:10.1016/j.mad.2012.01.006 · Cited by 38 · see citing works
Park, G., Kim, H., Kim, Y.-O., Park, S. H., Kim, S., & Oh, M. S. (2012) · Skin Pharmacology and Physiology · doi:10.1159/000335257 · Cited by 36 · see citing works
Self-citationLee, E. K., Kim, J. A., Park, S., et al. (2013) · BMB Reports · doi:10.5483/bmbrep.2013.46.5.199 · Cited by 36 · see citing works
Chang, S. W., Lee, S.-I., Bae, W., Min, K., Shin, E.-S., Oh, G., Pae, H., & Kim, E.-C. (2009) · Journal of Endodontics · doi:10.1016/j.joen.2009.06.005 · Cited by 33 · see citing works
Park, G., Kim, H. G., Hong, S., Kim, S. Y., & Oh, M. S. (2014) · Skin Pharmacology and Physiology · doi:10.1159/000354917 · Cited by 29 · see citing works
Self-citationPark, G., Kim, H. G., Sim, Y., Sung, S. H., & Oh, M. S. (2013) · Biological and Pharmaceutical Bulletin · doi:10.1248/bpb.b13-00101 · Cited by 29 · see citing works
Self-citationAnti-wrinkle effect of bone morphogenetic protein receptor 1a-extracellular domain (BMPR1a-ECD)
Yoon, B. H., Jeon, Y. H., Hwang, B., Kwon, H. N., Choe, S., & Yang, Z. Y. (2013) · BMB Reports · doi:10.5483/bmbrep.2013.46.9.238 · Cited by 5 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Skin aging and oxidative stress: Equol’s anti-aging effects via biochemical and molecular mechanisms
Lephart, E. D. (2016) · Ageing Research Reviews · doi:10.1016/j.arr.2016.08.001 · Cited by 325 · see citing works
- Authors
- Park, G., & Oh, M. S.
- Affiliations
- Kyung Hee University (KR)
- Corresponding author
- Oh, Myung Sook
- Venue
- BMB Reports
- Published
- 2015-08-31
- Volume
- 48
- Issue
- 8
- Pages
- 467-472
- ISSN
- 1976-6696 (ISSN-L)
- PMC
- PMC4576955
- OpenAlex
- W2337166170
- Cited by
- 12
- References
- 25
- Citations by year
- 2026: 1 · 2025: 1 · 2018: 2 · 2017: 1 · 2016: 7
- FWCI
- 1.45
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by-nc
- Locations
- BMB Reports (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Funders
- Korea Institute of Oriental Medicine (K14301)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics, phytochemicals, and oxidative stress
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Heat shock proteins research
- Keywords
- heat shock, Nrf2, collagen degradation, dermal fibroblasts, skin aging, antioxidant response, heme oxygenase-1, NQO1, glutathione, procollagen, Nrf2 knockdown
- MeSH terms
- Antioxidants, Cell Line, Collagen, Fibroblasts, Glutathione, Humans, Down-Regulation, Skin Aging, NAD(P)H Dehydrogenase (Quinone), Oxidative Stress, Heat-Shock Response, NF-E2-Related Factor 2, Heme Oxygenase-1, Gene Knockdown Techniques
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
