Cited by 28Enzyme Production and CharacterizationAdvanced Scientific Research MethodsEnzyme Catalysis and Immobilization

Production of milk-clotting enzyme by Bacillus subtilis B1 from wheat bran

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Zhongyang, Ding, Shuangping, Liu, Zhenghua, Gu, Liang, Zhang, Kechang, Zhang, Guiyang, Shi·2011·AFRICAN JOURNAL OF BIOTECHNOLOGY

Abstract

Three strains, Bacillus subtilis B1, B. subtilis B18 and Bacillus thuringiensis B12,were screened from wheat bran to produce milk-clotting enzyme. Among them, B. subtilis B1 exhibited considerable milk-clotting activity with low proteolytic activity. After response surface methodology optimization, milk-clotting activity wasimproved from 782 SU ml-1 to 1129.05 ± 74.55 SU mL-1 with a small inoculum size (0.130%, v/v). The optimized medium contained glucose (16.2 gL-1), wheat bran (30 gL-1), NaCl (5 gL-1), MgSO4·7H2O (5 gL-1), KH2PO4 (2 gL-1) and CaCO3 (3 gL-1). The milk-clotting enzyme from B. subtilis B1 has the optimum pH 5.5 and CaCl2concentration 50 mmol L-1. It is completely inactivated after 5 min at 70°C. The result showed that B. subtilis B1 was a promising strain for industrial milk-clotting enzyme production. Key words: Milk-clotting enzyme, Bacillus subtilis, submerged fermentation, wheat bran.

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Crossref · 10.5897/ajb10.1647

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  • Reduced MCE production by B. subtilis at high salt concentrations could be attributed to the sensitivity of the bacteria to salt and its limited capacity to synthesize MCE under such conditions.

    “Reduced MCE production by B. subtilis at high salt concentrations could be attributed to the sensitivity of the bacteria to salt and its limited capacity to synthesize MCE under such conditions (Zhongyang et al., 2011).”

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Authors
Zhongyang, D., Shuangping, L., Zhenghua, G., Liang, Z., Kechang, Z., & Guiyang, S.
Venue
AFRICAN JOURNAL OF BIOTECHNOLOGY
Published
2011-08-22
Volume
10
Issue
46
Pages
9370-9378
ISSN
1684-5315 (ISSN-L)
OpenAlex
W2116770624
Cited by
28
References
25
Citations by year
2026: 1 · 2025: 1 · 2024: 2 · 2021: 3 · 2020: 3 · 2019: 3 · 2018: 1 · 2017: 2 · 2016: 5 · 2014: 1 · 2013: 4 · 2012: 1
FWCI
1.91
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
AFRICAN JOURNAL OF BIOTECHNOLOGY (publishedVersion, cc-by)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Advanced Scientific Research Methods
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
Keywords
milk-clotting enzyme, Bacillus subtilis, wheat bran, submerged fermentation, enzyme production optimization, Bacillus thuringiensis, proteolytic activity, response surface methodology, fermentation medium optimization, enzyme characterization, industrial enzyme production

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