Cited by 434Plant tissue culture and regenerationPlant Molecular Biology ResearchPlant nutrient uptake and metabolism

The DOI matches its Crossref record; 116 of the 116 references it cites were found in a registry or an index; 9 of the 220 sources its claims cite were checked for support.

A handbook of plant tissue culture, by Philip R. White...

Open access

Abstract

a wound in a "normal" individual take part in an orderly granulation, closing the wound, form- ing well-limited scar tissue, organizing a harmonious replacement of the injured regions, while in an individual carrying a cancer, even at some distant part of the body, cells of the same region may sion.If, on the other hand, there has been a true segregation and loss of function so that the cells are, in the mature organism, no longer totipotent, * Italic numbers refer to bibliography.roots (center), or merely wound callus (right), depending on the spatial orientation of the cut, showing the dependence of morphogenetic expression on fortuitous external factors.(From Goebel, K. 1908.Einleitung in die experimentelle Morphologie der Pflanzen.220, fig.Ill, 293.) to grow cells and small groups of cells in suitable nutrients and to study their behavior.The dis- cipline which Haberlandt thus outlined is that which, in the four decades since his paper, has gradually crystallized into what we know today like a comparable degree.Inthe first place, ani- mal cells throughout the body are regularly bathed in one or both of two characteristic and essentially complete nutrient fluids, the blood and lymph.These can be easily removed from the body in large quantity.They serve as basic "natural" nutrients for animal cells in which such cells can be immersed without serious shock.They can be analyzed at leisure to determine which of their constituents are truly essential.In the second /'hint Tissue Cult/ire **Msl8& Pig. 3. Segments of larvae of Bana palustris (left) and R. sylvatica (right) having differenl pigmentation, grafted together in the early embryonic stages.Each retains its own specific char- acteristics in intimate anatomical and physiological contacl with the other.Al the right, progressive stages in development.At the upper left, histological detail of the region of union between the two.Compare with Fig. 2. (From Earrison, R. G. L904.Ark.

Overview

AI snapshot

Written from the paper’s full text.

TL;DR

White’s 1943 handbook reviews the history and methods of plant tissue culture, arguing that excised plant cells/tissues can be grown under controlled conditions and used to study nutrition, physiology, pathology, and morphogenesis. It is primarily a methodological synthesis, not a primary experimental report.

Key findings

  • Plant tissue culture history is divided into four periods: 1834–1900, 1900–1922, 1922–1934, and 1934–1939; White dates the first prolonged cultivation of excised roots to 1934, satisfactory synthetic media to 1937, and prolonged cultivation of undifferentiated plant tissues to 1939.
  • Routine excised tomato root cultures averaged about 7–8 mm growth per culture per day; optimum temperature was about 27°C for wheat roots and nearer 30°C for tomato, with a 5°C rise above optimum capable of killing cultures if maintained.
  • For root experiments, 20 replications per condition were standard; differences up to 12% were not statistically significant, 24–25% were doubtful, and 31% or more were highly significant.
  • Linear increment measurements ignoring branches erred by about 5–8% compared with dry weights, while measurement itself had a mean error of 6–8%.

Methods

  • Design: a handbook/review that synthesizes historical literature and laboratory procedures for plant tissue culture, including root tips, callus, cambium, procambium, and embryos.
  • Data/sample: cited experiments and the author’s laboratory protocols, with quantitative examples from excised root cultures and nutrient studies.
  • Main analysis: narrative synthesis and methodological prescription, including nutrient formulations, sterilization, culture techniques, growth measurement, replication, and statistical interpretation.

Limitations

  • Stated: the field is “still too new to be frozen into definite patterns,” and the account “merely attempts to bring it as nearly up to date as possible”; many fields remained untouched.
  • Evident from the text: it is a 1943 handbook whose coverage ends in the early 1940s and relies on literature and laboratory practice rather than a single systematic experimental study.
  • Evident from the provided text: the OCR is fragmentary and contains missing or duplicated passages, so some details are incomplete.

Model-written summary. Check it against the paper before relying on it.

Integrity signals

Registry

Crossref · 10.5962/bhl.title.4656

Cites

116 of 116 references verified0 citation errors · 0 not checkable

Statistics

Nothing to recompute.

Verification

Claims the paper makes

98 Own interpretation · 84 No basis given · 63 Sources not found · 24 Not checked yet · 23 No full text of the sources · 20 Own result · 3 Supported so far · 3 Partly supported · 3 Own method · 1 Supported · 1 Not supported · accuracy not yet measured

Show all 134 claims

  • Probably not a substantial claim. 101
  • It may state two separate claims as one. 127
  • The quote only partly states this claim. 1
189 of 189
  • The paper relies on this without support.

    The cell is theoretically the ultimate unit of structure in living organisms.

    “Since the cell is theoretically the ultimate unit of structure in liv- ing organisms, the question of the origin of form and function in living beings may be stated thus”
  • The paper relies on this without support.

    No mechanism is known by which segregation or loss of totipotency can take place.

    “No mechanism is known by which such segregation or loss can take place, yet it so often appears to have taken place that this alternative hypothesis has today many adherents.”
  • The paper relies on this without support.

    Cellular totipotency is a fundamental property of plant cells.

    “there can be no question about its fundamental totipotency”
  • The paper relies on this without support.

    The general requirements for a plant tissue culture laboratory are the same as those for any detailed micro-culture work.

    “The gen- eral requirements for a plant tissue culture lab- oratory are the same as those for any detailed micro-culture work”
  • The paper relies on this without support.

    Sharing a media room with bacteriologists, mycologists, etc., involves definite risks of contaminations, both biological and chemical.

    “since sharing it with bacteriologists, mycologists, etc., involves definite risks of contaminations, both biological and chemical.”
  • The paper relies on this without support.

    Molds are likely to grow in cotton plugs and ultimately penetrate to the nutrient if plugged flasks are not covered.

    “else molds are likely to grow in the plugs and ultimately penetrate to the nutrient”
  • The paper relies on this without support.

    Cotton plugs char and bits of charred cotton falling into flasks of nutrient may vitiate the results of nutrition experiments.

    “Cotton plugs char, and bits of charred cotton falling into flasks of nutrient may vitiate the results of nutrition experiments.”
  • The paper relies on this without support.

    Flasks which are properly handled and plugged and are capped to keep out dust and water of condensation will remain sterile for months without flaming.

    “Flasks which are properly handled and plugged and are capped to keep out dust and water of condensation will remain sterile for months without flaming.”
  • The paper relies on this without support.

    Instruments can be adequately sterilized in boiling water.

    “Instruments can be ade- quately sterilized in boiling water.”
  • The paper relies on this without support.

    Seeds contained in very small fruits, in drupes or achenes, or the seeds of Gymnosperms cannot be obtained in a uniformly aseptic condition by mechanical manipulation alone.

    “Seeds contained in very small fruits, in drupes or achenes, or the seeds of Gymnosperms cannot be obtained in a uniformly aseptic condition by mechanical manipulation alone.”
  • The paper relies on this without support.

    When seeds are sown on a semi-solid substratum such as agar, any contaminant present on one seed will not easily spread to another.

    “If sown on a semi-solid substratum such as agar, several seeds can be placed in each receptacle since any contaminant which may be present on one seed will not easily spread to another.”
  • The paper relies on this without support.

    True science rests upon quantitative representations.

    “True science rests upon quantitative representa- tions.”
  • The paper relies on this without support.

    Living organisms, particularly plant materials incapable of autonomous movement, give evidence of their reactions to changes in environment chiefly by changes in character or velocity of growth.

    “Living organisms, particularly those which, like most plant materials, are incapable of autonomous movement, give evidence of their reactions to changes in environment chiefly by changes in char- acter or velocity of growth.”
  • The paper relies on this without support.

    Living materials are chiefly proteins.

    “These living materials are chiefly proteins.”
  • The paper relies on this without support.

    Carbohydrates and fats and non-protein nitrogenous materials are largely constituents that can be removed without destroying the organism's capacity to live.

    “The carbohydrates and fats and the nitrogenous materials of non-protein character are largely constituents which can be removed without destroying the organism's ca- pacity to live.”
  • The paper relies on this without support.

    The significance of any piece of scientific work rests primarily on the ability to express results in quantitative terms and the reliability of these terms themselves.

    “The significance of any piece of scientific work rests primarily on the ability to express results in quantitative terms and the reliability of these terms themselves.”
  • The paper relies on this without support.

    All cultures react on average in approximately the same way and to the same extent to most variables in the environment.

    “Since all cultures react on the average in approximately the same way and to the same extent to most variables in the environment”
  • The paper relies on this without support.

    Varietal differences or uncontrolled seasonal variables in the environment may have as great or greater effects on growth rates than do experimental variables.

    “the ef- fects of varietal differences or of uncontrolled seasonal variables in the environment may have as great or greater effects on growth rates than do the experimental variables.”
  • The paper relies on this without support.

    The basic nutrient for tissues is also an excellent nutrient for many pathogens.

    “for the obvious reason that the basic nutrient for tissues is also an excellent nutrient for many of these organisms.”
19 of 19

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

Each contradiction between two passages listed here was confirmed by an independent check.

1 of 1

Citations

Cites

Show all 110 references

Research works printed but not linked: 13

  1. Borger, H. 1926. Verfahren pflanzlicher Gewebeziichtung. (Nachtrag zur: Erdmann, Rh. Gewebeziichtung.) Handb. norm. path. Physiol., Band 14, l.Halfte: 1000-1002. Springer, Berlin.
  2. Ephrussi, B. 1932. La culture des tissus. pp. 233.
  3. 1930. Praktikum der Gewebepflege oder Explan- tation besonders der Gewebeziiehtung. Ed. 2. Springer, Berlin.
  4. Gautheret, R. 1935. Kecherches sur la culture des tissus vegetaux: Essais de culture de quelques tissus me>i- stSmatiques. These, Univ. Paris, pp. 279.
  5. 1937. La culture des tissus vegetaux. Son 6tat actuel, comparaison avec la culture des tissus animaux. Actuality sci. industrielles. pp. 66. Hermann, Paris.
  6. Lewis, W. H., and Lewis, M. R. 1924. Behavior of cells in tissue cultures. (In: Cowdry, General cytology, Univ. Chicago Press, pp. 385-447.)
  7. Loeb, J. 1916. The organism as a whole, pp. 379. G. P. Putnam's Son, New York.
  8. 1924. Regeneration from a physicochemical view point, pp. 143. McGraw-Hill Book Co., Inc., New York.
  9. 1939. Plant organ and tissue physiology, as ex- emplified in growing isolated roots. Papers presented before Am. Sci. Teachers Assoc, A.A.A.S., December, . 1938. pp. 15-22.
  10. Galligar, G. C. 1936. Influence of certain organic sub- stances upon the growth behaviour of excised root tips. Trans. Illinois Acad. Sci. 29 : 59-68. 167. . 1938. Correlation between growth of excised root tips and types of food stored in the seed. Plant Physiol. 13: 599-609. 168. Knudson, L. 1916. Influence of certain carbohydrates on green plants. Cornell Univ. Agr. Exp. Sta. Memoir 9. pp. 75. 169. , and Smith, E. S. 1919. Secretion of amylase by plant roots. Bot. Gaz. 68: 460-466. 170. Bobbins, W. J., and Bartley, M. A. 1937. Use of dextrose by excised tomato roots. Science 86: 290-291. 171.
  11. Delarge, L. 1938. Cultures de meristemes radiculaires in vitro. Les variations individuelles et leur signification pbysiologiques. Bull. soc. roy. bot. belg. 71: 73-88. 281. . 1939. Cultures de meristemes radiculaires in vitro. Les milieux reputes complets et les variations indi- viduelles. Bull. soc. roy. bot. belg. 71 : 145-162. 282. Gautheret, R. 1937. Sur la migration cellulaire dans les cultures d'organes et de tissus vegetaux. C. r. soc. biol., Paris, 124: 632-634. 283. . 1938. Caracteres anatomiques de fragments de tubercules de carotte cultives in vitro. C. r. soc. biol., Paris, 127: 259-261. 284. . 1938. Caracteres cytologiques de tranches de tubercules de carotte cultives in vitro. C. r. soc. biol., Paris, 127 : 609-612. 285. . 1939. Sur la mesure de la croissance des tissus de carotte cultives in vitro. C. r. acad. sci., Paris, 208: 1340. 286. . 1940. Eecherches sur le bourgeonnement du tissu cambial d'Ulmus campestris cultiv6 in vitro. C. r. acad. sci., Paris, 210: 632-634. 287. . 1940. Nouvelles recherches sur le bourgeonne- ment du tissu cambial d 'Ulmus campestris cultivS in vitro. C. r. acad. sci., Paris, 210: 744-746. 288. . 1940. Recherches experimentales sur la polarity des tissus du tubercule de Carotte. C. r. acad. sci., Paris, 211: 15-18. 289. . 1940. Remarques sur la structure des tissus de carotte cultives in vitro. C. r. soc. biol., Paris, 134: 237- 238. 290. Gioelli, F. 1937. II differenziamento citologico ed isto- logico dei meristemi cambiali in vitro. (Nota I.) Accad. sei. Ferrara, atti 1937, vol. 14, 2' ser.: 172-174. 291. . 1937. Influenza della temperatura e della luce sullo sviluppo dei meristemi cambiali in vitro. (Nota II.)
  12. Kiister, E. 1911. Die Gallen der Pflanzen. pp. 437. Hir- zel Verlag, Leipzig. 301.
  13. White, P. R. 1934. Multiplication of the viruses of tobacco and aucuba mosaic in growing excised tomato roots. Phytopath. 24: 1003-1011. 439. . 1936. The cultivation of plant disease viruses in actively growing excised plant organs in vitro. Proc. 2nd Int. Congress Microbiol., London, pp. 30-31. Pathology -Animal 440.

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Metadata

Authors
White, P. R.
Corresponding author
White, Philip R.
Published
1943
OpenAlex
W2493594252
Cited by
434
References
110
Citations by year
2023: 1 · 2022: 2 · 2021: 1 · 2020: 2 · 2019: 3 · 2018: 3 · 2017: 4 · 2016: 4 · 2015: 2 · 2014: 1 · 2013: 1 · 2012: 3
FWCI
9.53
Type
book
Language
en
Open access
Locations
Biodiversity Heritage Library (Smithsonian Institution) (submittedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant tissue culture and regeneration
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
Keywords
plant tissue culture, plant cell culture, totipotency, morphogenesis, wound healing, grafting

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