Dental pulp stem cells: a new cellular resource for corneal stromal regeneration
Open accessAbstract
Corneal blindness afflicts millions of individuals worldwide and is currently treated by grafting with cadaveric tissues; however, there are worldwide donor tissue shortages, and many allogeneic grafts are eventually rejected. Autologous stem cells present a prospect for personalized regenerative medicine and an alternative to cadaveric tissue grafts. Dental pulp contains a population of adult stem cells and, similar to corneal stroma, develops embryonically from the cranial neural crest. We report that adult dental pulp cells (DPCs) isolated from third molars have the capability to differentiate into keratocytes, cells of the corneal stoma. After inducing differentiation in vitro, DPCs expressed molecules characteristic of keratocytes, keratocan, and keratan sulfate proteoglycans at both the gene and the protein levels. DPCs cultured on aligned nanofiber substrates generated tissue-engineered, corneal stromal-like constructs, recapitulating the tightly packed, aligned, parallel fibrillar collagen of native stromal tissue. After injection in vivo into mouse corneal stroma, human DPCs produced corneal stromal extracellular matrix containing human type I collagen and keratocan and did not affect corneal transparency or induce immunological rejection. These findings demonstrate a potential for the clinical application of DPCs in cellular or tissue engineering therapies for corneal stromal blindness.
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In animals, corneal stem cells can restore tissue transparency.
“In animals, it has previously been demonstrated how corneal stem cells can restore tissue transparency (Du et al., 2009, Syed-Picard et al., 2015).”
From Corneal structure and transparency (2015) · cites this paper as Syed-Picard et al., 2015
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Human mesenchymal stem cells modulate allogeneic immune cell responses
Aggarwal, S., & Pittenger, M. F. (2005) · Blood · doi:10.1182/blood-2004-04-1559 · Cited by 4,511 · see citing works
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Corneal structure and transparency
Meek, K. M., & Knupp, C. (2015) · Progress in Retinal and Eye Research · doi:10.1016/j.preteyeres.2015.07.001 · Cited by 853 · see citing works
- Authors
- Syed-Picard, F. N., Du, Y., Lathrop, K. L., Mann, M. M., Funderburgh, M. L., & Funderburgh, J. L.
- Affiliations
- University of Pittsburgh (US)
- Corresponding author
- Funderburgh, James L.
- Venue
- Stem Cells Translational Medicine
- Publisher
- Oxford University Press (OUP)
- Published
- 2015-02-11
- Volume
- 4
- Issue
- 3
- Pages
- 276-285
- ISSN
- 2157-6564 (ISSN-L)
- 2157-6580
- PMC
- PMC4339846
- OpenAlex
- W2060944529
- Cited by
- 106
- References
- 1
- Citations by year
- 2025: 3 · 2024: 7 · 2023: 12 · 2022: 10 · 2021: 17 · 2020: 12 · 2019: 11 · 2018: 7 · 2017: 10 · 2016: 12 · 2015: 5
- FWCI
- 8.35
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Stem Cells Translational Medicine (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- NIH (EY016415, EY009368, P30-EY008098)
- Topics
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Corneal Surgery and Treatments
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Keywords
- dental pulp stem cells, corneal stroma regeneration, keratocyte differentiation, keratocan, keratan sulfate proteoglycan, type I collagen, corneal blindness, autologous stem cell therapy, extracellular matrix, cranial neural crest
- MeSH terms
- Adult, Animals, Blindness, Cell Differentiation, Cornea, Corneal Diseases, Dental Pulp, Humans, Stem Cells, Stem Cell Transplantation, Mice, Heterografts
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