Comparison of wound healing effects between Oncorhynchus keta-derived polydeoxyribonucleotide (PDRN) and Oncorhynchus mykiss-derived PDRN
Open accessAbstract
Comparison of wound healing effects between Oncorhynchus keta-derived polydeoxyribonucleotide (PDRN) and Oncorhynchus mykiss-derived PDRNBackground: Polydeoxyribonucleotide (PDRN) influencing cellular growth and differentiation is recognized to promote wound healing by stimulating tissue repair.Although PDRN can be extracted from human placentas, PDRN medications have recently been extracted from the semen of trout (Oncorhynchus mykiss) and salmon (Oncorhynchus keta).The present study was designed to evaluate the wound healing effects of O. keta -derived PDRN for injection (Rejuvenex) and PDRN cream (Rejuvenex Cream) in comparison with those of O. mykiss-derived PDRN injection (Placentex).Methods: Full-thickness skin defects were made on the back of mice (n=60).The mice were divided into the following four groups according to the dressing used for the wounds: O. mykiss-derived PDRN injection group, O. keta-derived PDRN injection group, O. ketaderived PDRN cream group, and normal saline soaked dressing group (control group).We analyzed the gross findings, wound sizes, histological findings, immunohistochemistry and enzyme-linked immunosorbent assays for the groups immediately after the treatment, and again after 4, 7, and 10 days of treatment.Results: The wound healing effects were the greatest in the O. keta-derived PDRN injection and O. mykiss-derived PDRN injection groups, which showed similar scores, followed by the O. keta-derived cream and normal saline soaked dressing groups.Conclusion: The injection of PDRN extracted from O. keta was found to be as effective at healing full-thickness skin defects as the O. mykiss-derived PDRN injection, which is currently used in the clinic.Moreover, the O. keta-derived PDRN injection was also found to reduce the time required for wound healing.
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Placentex® is manufactured using a proprietary High Purification Technology (HPT) designed to enhance purity and partially control the polymer length.
“This product is manufactured using a proprietary High Purification Technology (HPT) designed to enhance purity and partially control the polymer length [165,166].”
From Comparison of Polynucleotide and Polydeoxyribonucleotide in Dermatology: Molecular Mechanisms and Clinical Perspectives (2025) · cites this paper as [165]
The Introduction (page unavailable) says PDRN medications are obtained by high-temperature extraction, have purity above 95%, and comprise polymers of 50–2,000 base pairs. The Methods—Materials section identifies Placentex as a PDRN injection. Neither section says Placentex is manufactured using proprietary High Purification Technology (HPT) or that its manufacturing process controls polymer length. (LLM, conf=0.84)
Experimental — accuracy not yet measuredPlacentex® is a PDRN-based therapeutic developed by Mastelli S.R.L. in Italy.
“A notable example is Placentex®, a PDRN-based therapeutic developed by Mastelli S.R.L. in Italy.”
From Comparison of Polynucleotide and Polydeoxyribonucleotide in Dermatology: Molecular Mechanisms and Clinical Perspectives (2025) · cites this paper as [165]
A PN-based medical device incorporating HPT®, developed by Mastelli S.R.L., significantly improved facial wrinkles and skin roughness, as measured using three-dimensional skin imaging and the global esthetic improvement scale (GAIS).
“The use of a PN-based medical device incorporating HPT®, developed by Mastelli S.R.L., significantly improved facial wrinkles and skin roughness, as measured using three-dimensional skin imaging and the global esthetic improvement scale (GAIS) [165,166].”
From Comparison of Polynucleotide and Polydeoxyribonucleotide in Dermatology: Molecular Mechanisms and Clinical Perspectives (2025) · cites this paper as [165]
The Methods and Discussion (pages not provided) describe PDRN treatment of full-thickness wounds on mice, assessed by biopsy and wound-healing measures. Searches for HPT®, Mastelli, GAIS, facial wrinkles, skin roughness, and three-dimensional skin imaging found no passage addressing the claimed device or cosmetic outcomes. (LLM, conf=0.78)
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Cited by
Sorted by citations
Colangelo, M. T., Galli, C., & Guizzardi, S. (2020) · Regenerative Medicine · doi:10.2217/rme-2019-0118 · Cited by 59 · see citing works
Jo, S., Baek, A., Cho, Y., Kim, S. H., Baek, D., Hwang, J., Cho, S.-R., & Kim, H. J. (2023) · Scientific Reports · doi:10.1038/s41598-023-32744-9 · Cited by 19 · see citing works
Kim, S. T. (2025) · Pharmaceutics · doi:10.3390/pharmaceutics17081024 · Cited by 18 · see citing works
- Authors
- Lee, J. H., Han, J. W., Byun, J. H., Lee, W. M., Kim, M. H., & Wu, W. H.
- Affiliations
- Eulji University (KR)
- Corresponding author
- Lee, Jong Hun
- Han, Jin Woo
- Venue
- Archives of Craniofacial Surgery
- Published
- 2018-03-20
- Volume
- 19
- Issue
- 1
- Pages
- 20-34
- ISSN
- 2287-1152 (ISSN-L)
- 2287-5603
- PMC
- PMC5894545
- OpenAlex
- W2795142336
- Cited by
- 29
- References
- 0
- Citations by year
- 2026: 5 · 2025: 7 · 2024: 3 · 2023: 2 · 2022: 3 · 2021: 3 · 2020: 2 · 2019: 3 · 2018: 1
- FWCI
- 1.92
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Licence
- Locations
- Archives of Craniofacial Surgery (publishedVersion, cc-by-nc)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Funders
- Eulji University (13-023-411)
- Topics
- Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
- Health Sciences › Medicine › Urology › Periodontal Regeneration and Treatments
- Health Sciences › Medicine › Dermatology › Dermatologic Treatments and Research
- Keywords
- polydeoxyribonucleotide, wound healing, chum salmon, rainbow trout, full-thickness skin defect, tissue repair, injection therapy
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