Therapeutic effects of polydeoxyribonucleotide in an in vitro neuronal model of ischemia/reperfusion injury
Open accessAbstract
Polydeoxyribonucleotide (PDRN) is an agonist that selectively stimulates adenosine A 2A receptor (ADORA2A), which suppresses inflammatory responses. Ischemia/reperfusion (I/R) injury plays a major role in the pathogenesis of ischemic stroke by inducing neuroinflammation. Therefore, this study aimed to investigate the therapeutic effects of PDRN in an in vitro I/R injury model. The in vitro model was established with differentiated Neuro-2a cells under oxygen and glucose deprivation condition. The cells were treated with PDRN for 24 h under reoxygenation condition. As the results of RNA-seq transcriptome analysis, CSF1, IL-6, PTPN6, RAC2, and STAT1 were identified of its relation to the effect of PDRN on inflammatory responses in the model. To further investigate therapeutic effects of PDRN, RT-qPCR, western blotting, LDH assay, and TUNEL assay were performed. PDRN significantly reversed the expression of genes and proteins related to inflammatory responses. The elevated ADORA2A expression by PDRN treatment downregulated JAK/STAT pathway in the model. Furthermore, PDRN inhibited neuronal cell death in the model. Consequently, our results suggested that PDRN alleviated inflammatory responses through inhibition of JAK/STAT pathway by mediating ADORA2A expression and inhibited neuronal cell death in the model. These results provide significant insights into potential therapeutic approaches involving PDRN treatment for I/R injury.
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PDRNs are classified as prescription medications approved for injectable use for wound healing/tissue repair and knee osteoarthritis.
“PDRNs are classified as prescription medications approved for injectable use for wound healing/tissue repair and knee osteoarthritis [19,173,174].”
From Comparison of Polynucleotide and Polydeoxyribonucleotide in Dermatology: Molecular Mechanisms and Clinical Perspectives (2025) · cites this paper as [174]
The Results section describes PDRN as a drug used to treat osteoarthritis, and the References list studies of wound healing and intra-articular injections for knee osteoarthritis (no page numbers available). The paper does not say PDRNs are classified as prescription medications or approved for injectable use for either indication. (LLM, conf=0.84)
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- Authors
- Jo, S., Baek, A., Cho, Y., Kim, S. H., Baek, D., Hwang, J., Cho, S.-R., & Kim, H. J.
- Affiliations
- Yonsei University (KR)
- Eulji University (KR)
- Corresponding author
- Kim, Sung Hoon
- Cho, Sung-Rae
- Kim, Hyun Jung
- Venue
- Scientific Reports
- Published
- 2023-04-12
- Volume
- 13
- Issue
- 1
- Pages
- 6004-6004
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC10097812
- OpenAlex
- W4365151359
- Cited by
- 19
- References
- 34
- Citations by year
- 2026: 6 · 2025: 8 · 2024: 5
- FWCI
- 1.96
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- YUHSpace (Yonsei University Medical Library) (submittedVersion, cc-by-nc-nd)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Hyundai Motor Chung Mong-Koo Foundation
- National Research Foundation, South Korea (NRF-2022R1A2C1006374)
- Student Research Bursary of Yonsei University College of Medicine (2020)
- Korean Fund for Regenerative Medicine (21A0202L1, 21C0715L1)
- Korea Health Industry Development Institute (HI21C1314, HI22C1588)
- Eulji University (2020)
- Topics
- Life Sciences › Neuroscience › Neurology › Neurological Disorders and Treatments
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Health Sciences › Medicine › Neurology › Traumatic Brain Injury and Neurovascular Disturbances
- Keywords
- polydeoxyribonucleotide, ischemia-reperfusion injury, neuroinflammation, adenosine A2A receptor, JAK/STAT signaling, neuronal cell death, ischemic stroke, oxygen-glucose deprivation, N2a cells, CSF-1, interleukin-6, STAT1, SHP-1, Rac2
- MeSH terms
- Humans, Ischemia, Polydeoxyribonucleotides, Signal Transduction, Reperfusion Injury, STAT Transcription Factors, Janus Kinases
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