The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Piezo1 links mechanical forces to red blood cell volume
Open accessAbstract
Red blood cells (RBCs) experience significant mechanical forces while recirculating, but the consequences of these forces are not fully understood. Recent work has shown that gain-of-function mutations in mechanically activated Piezo1 cation channels are associated with the dehydrating RBC disease xerocytosis, implicating a role of mechanotransduction in RBC volume regulation. However, the mechanisms by which these mutations result in RBC dehydration are unknown. In this study, we show that RBCs exhibit robust calcium entry in response to mechanical stretch and that this entry is dependent on Piezo1 expression. Furthermore, RBCs from blood-cell-specific Piezo1 conditional knockout mice are overhydrated and exhibit increased fragility both in vitro and in vivo. Finally, we show that Yoda1, a chemical activator of Piezo1, causes calcium influx and subsequent dehydration of RBCs via downstream activation of the KCa3.1 Gardos channel, directly implicating Piezo1 signaling in RBC volume control. Therefore, mechanically activated Piezo1 plays an essential role in RBC volume homeostasis.
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Cites
Fiji: an open-source platform for biological-image analysis
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Piezo1 and Piezo2 Are Essential Components of Distinct Mechanically Activated Cation Channels
Coste, B., Mathur, J., Schmidt, M., Earley, T. J., Ranade, S., Petrus, M. J., Dubin, A. E., & Patapoutian, A. (2010) · Science · doi:10.1126/science.1193270 · Cited by 3,316
Self-citationPiezo1 integration of vascular architecture with physiological force
Li, J., Hou, B., Tumova, S., Muraki, K., Bruns, A., Ludlow, M. J., Sedo, A., Hyman, A. J., McKeown, L., Young, R. S., et al. (2014) · Nature · doi:10.1038/nature13701 · Cited by 1,223
Piezo proteins are pore-forming subunits of mechanically activated channels
Coste, B., Xiao, B., Santos, J. S., Syeda, R., Grandl, J., Spencer, K. S., Kim, S. E., Schmidt, M., Mathur, J., Dubin, A. E., et al. (2012) · Nature · doi:10.1038/nature10812 · Cited by 1,167
Self-citationPiezo1, a mechanically activated ion channel, is required for vascular development in mice
Ranade, S. S., Qiu, Z., Woo, S.-H., Hur, S. S., Murthy, S. E., Cahalan, S. M., Xu, J., Mathur, J., Bandell, M., Coste, B., et al. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1409233111 · Cited by 901
Self-citationChemical activation of the mechanotransduction channel Piezo1
Syeda, R., Xu, J., Dubin, A. E., Coste, B., Mathur, J., Huynh, T., Matzen, J., Lao, J., Tully, D. C., Engels, I. H., et al. (2015) · eLife · doi:10.7554/elife.07369 · Cited by 737
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Self-citationMutations in the mechanotransduction protein PIEZO1 are associated with hereditary xerocytosis
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Cited by
Mechanically Activated Ion Channels
Ranade, S. S., Syeda, R., & Patapoutian, A. (2015) · Neuron · doi:10.1016/j.neuron.2015.08.032 · Cited by 652
Self-citationMechanically activated ion channel Piezo1 modulates macrophage polarization and stiffness sensing
Atcha, H., Jairaman, A., Holt, J. R., Meli, V. S., Nagalla, R. R., Veerasubramanian, P. K., Brumm, K. T., Lim, H. E., Othy, S., Cahalan, M. D., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-23482-5 · Cited by 644
Endothelial cation channel PIEZO1 controls blood pressure by mediating flow-induced ATP release
Wang, S., Chennupati, R., Kaur, H., Iring, A., Wettschureck, N., & Offermanns, S. (2016) · Journal of Clinical Investigation · doi:10.1172/jci87343 · Cited by 616
Piezos thrive under pressure: mechanically activated ion channels in health and disease
Murthy, S. E., Dubin, A. E., & Patapoutian, A. (2017) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2017.92 · Cited by 582
Self-citationStructure-based membrane dome mechanism for Piezo mechanosensitivity
Guo, Y. R., & MacKinnon, R. (2017) · eLife · doi:10.7554/elife.33660 · Cited by 446
Metadata
- Authors
- Cahalan, S. M., Lukacs, V., Ranade, S. S., Chien, S., Bandell, M., & Patapoutian, A.
- Venue
- eLife
- Publisher
- eLife Sciences Publications, Ltd
- Published
- 2015-05-22
- Volume
- 4
- ISSN
- 2050-084X (ISSN-L)
- OpenAlex
- W2104989250
- Cited by
- 624
- References
- 26
- FWCI
- 22.17
- Type
- journal article
- Language
- en
- Open access
- gold
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- Cahalan, S. M., Lukacs, V., Ranade, S. S., Chien, S., Bandell, M., & Patapoutian, A. (2015). Piezo1 links mechanical forces to red blood cell volume. eLife. https://doi.org/10.7554/elife.07370
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