The DOI matches its Crossref record; 10 of the 10 references it cites were found in a registry or an index.
Operon mRNAs are organized into ORF-centric structures that predict translation efficiency
Open accessAbstract
Bacterial mRNAs are organized into operons consisting of discrete open reading frames (ORFs) in a single polycistronic mRNA. Individual ORFs on the mRNA are differentially translated, with rates varying as much as 100-fold. The signals controlling differential translation are poorly understood. Our genome-wide mRNA secondary structure analysis indicated that operonic mRNAs are comprised of ORF-wide units of secondary structure that vary across ORF boundaries such that adjacent ORFs on the same mRNA molecule are structurally distinct. ORF translation rate is strongly correlated with its mRNA structure in vivo, and correlation persists, albeit in a reduced form, with its structure when translation is inhibited and with that of in vitro refolded mRNA. These data suggest that intrinsic ORF mRNA structure encodes a rough blueprint for translation efficiency. This structure is then amplified by translation, in a self-reinforcing loop, to provide the structure that ultimately specifies the translation of each ORF.
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Cites
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Self-citationCoding-Sequence Determinants of Gene Expression in Escherichia coli
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In vivo genome-wide profiling of RNA secondary structure reveals novel regulatory features
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Self-citationTranslation efficiency is determined by both codon bias and folding energy
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“tAI incorporates different efficiency weights of the wobble interactions between codons and anticodons, with wi is defined as the relative adaptiveness value of codon i of a gene (Tuller et al., 2010).”
InfluentialCauses and effects of N-terminal codon bias in bacterial genes
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Landscape and variation of RNA secondary structure across the human transcriptome
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Functional 5′ UTR mRNA structures in eukaryotic translation regulation and how to find them
Leppek, K., Das, R., & Barna, M. (2018) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2017.103 · Cited by 949
Pervasive Regulatory Functions of mRNA Structure Revealed by High-Resolution SHAPE Probing
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Quantitative Control for Stoichiometric Protein Synthesis
Taggart, J. C., Lalanne, J.-B., & Li, G.-W. (2021) · Annual Review of Microbiology · doi:10.1146/annurev-micro-041921-012646 · Cited by 17
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Metadata
- Authors
- Burkhardt, D. H., Rouskin, S., Zhang, Y., Li, G.-W., Weissman, J. S., & Gross, C. A.
- Venue
- eLife
- Publisher
- eLife Sciences Publications, Ltd
- Published
- 2017-01-31
- Volume
- 6
- ISSN
- 2050-084X (ISSN-L)
- OpenAlex
- W2583817197
- Cited by
- 163
- References
- 10
- FWCI
- 6.89
- Type
- journal article
- Language
- en
- Open access
- gold
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