The DOI matches its Crossref record; 115 of the 115 references it cites were found in a registry or an index.
Pervasive Regulatory Functions of mRNA Structure Revealed by High-Resolution SHAPE Probing
Open accessAbstract
SUMMARY Messenger RNAs (mRNAs) can fold into complex structures that regulate gene expression. Resolving such structures de novo has remained challenging and has limited understanding of the prevalence and functions of mRNA structure. We use SHAPE-MaP experiments in living E. coli cells to derive quantitative, nucleotide-resolution structure models for 194 endogenous transcripts encompassing approximately 400 genes. Individual mRNAs have exceptionally diverse architectures, and most contain well-defined structures. Active translation destabilizes mRNA structure in cells. Nevertheless, mRNA structure remains similar between in-cell and cell-free environments, indicating broad potential for structure-mediated gene regulation. We find that translation efficiency of endogenous genes is regulated by unfolding kinetics of structures overlapping the ribosome binding site. We discover conserved structured elements in 35% of untranslated regions, several of which we validate as novel protein binding motifs. RNA structure regulates every gene studied here in a meaningful way, implying that most functional structures remain to be discovered.
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Saha, K., England, W., Fernandez, M. M., Biswas, T., Spitale, R. C., & Ghosh, G. (2020) · Nucleic Acids Research · doi:10.1093/nar/gkaa358 · Cited by 25
Wolfe, M. B., Schagat, T. L., Paulsen, M. T., Magnuson, B., Ljungman, M., Park, D., Zhang, C., Campbell, Z. T., Goldstrohm, A. C., & Freddolino, P. L. (2020) · RNA · doi:10.1261/rna.077362.120 · Cited by 22
Quantitative Control for Stoichiometric Protein Synthesis
Taggart, J. C., Lalanne, J.-B., & Li, G.-W. (2021) · Annual Review of Microbiology · doi:10.1146/annurev-micro-041921-012646 · Cited by 17
Szikszai, M., Wang, T.-Y., Krueger, R., Mathews, D. H., Ward, M., & Aviran, S. (2026) · RNA · doi:10.1261/rna.080846.125 · Cited by 5
Metadata
- Authors
- Mustoe, A. M., Busan, S., Rice, G. M., Hajdin, C. E., Peterson, B. K., Ruda, V. M., Kubica, N., Nutiu, R., Baryza, J. L., & Weeks, K. M.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2018-03
- Volume
- 173
- Issue
- 1
- Pages
- 181-195.e18
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2793097545
- Cited by
- 258
- References
- 115
- FWCI
- 11.93
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Mustoe, A. M., Busan, S., Rice, G. M., Hajdin, C. E., Peterson, B. K., Ruda, V. M., Kubica, N., Nutiu, R., Baryza, J. L., & Weeks, K. M. (2018). Pervasive Regulatory Functions of mRNA Structure Revealed by High-Resolution SHAPE Probing. Cell. https://doi.org/10.1016/j.cell.2018.02.034
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