The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Proximity labeling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID
Open accessAbstract
Defining specific protein interactions and spatially or temporally restricted local proteomes improves our understanding of all cellular processes, but obtaining such data is challenging, especially for rare proteins, cell types, or events. Proximity labeling enables discovery of protein neighborhoods defining functional complexes and/or organellar protein compositions. Recent technological improvements, namely two highly active biotin ligase variants (TurboID and miniTurbo), allowed us to address two challenging questions in plants: (1) what are in vivo partners of a low abundant key developmental transcription factor and (2) what is the nuclear proteome of a rare cell type? Proteins identified with FAMA-TurboID include known interactors of this stomatal transcription factor and novel proteins that could facilitate its activator and repressor functions. Directing TurboID to stomatal nuclei enabled purification of cell type- and subcellular compartment-specific proteins. Broad tests of TurboID and miniTurbo in Arabidopsis and Nicotiana benthamiana and versatile vectors enable customization by plant researchers.
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Fiji: an open-source platform for biological-image analysis
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REVIGO summarizes and visualizes long lists of gene ontology terms
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Proteomic Analysis of Unbounded Cellular Compartments: Synaptic Clefts
Loh, K. H., Stawski, P. S., Draycott, A. S., Udeshi, N. D., Lehrman, E. K., Wilton, D. K., Svinkina, T., Deerinck, T. J., Ellisman, M. H., Stevens, B., et al. (2016) · Cell · doi:10.1016/j.cell.2016.07.041 · Cited by 399
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Cited by
Deciphering molecular interactions by proximity labeling
Qin, W., Cho, K. F., Cavanagh, P. E., & Ting, A. Y. (2021) · Nature Methods · doi:10.1038/s41592-020-01010-5 · Cited by 671
Self-citationProximity labeling in mammalian cells with TurboID and split-TurboID
Cho, K. F., Branon, T. C., Udeshi, N. D., Myers, S. A., Carr, S. A., & Ting, A. Y. (2020) · Nature Protocols · doi:10.1038/s41596-020-0399-0 · Cited by 534
Self-citationHighly effective proximate labeling in Drosophila
Zhang, B., Zhang, Y., & Liu, J.-L. (2021) · G3 Genes|Genomes|Genetics · doi:10.1093/g3journal/jkab077 · Cited by 32
Barbosa, I. C. R., De Bellis, D., Flückiger, I., Bellani, E., Grangé-Guerment, M., Hématy, K., & Geldner, N. (2023) · Nature Communications · doi:10.1038/s41467-023-37265-7 · Cited by 28
Metadata
- Authors
- Mair, A., Xu, S.-L., Branon, T. C., Ting, A. Y., & Bergmann, D. C.
- Venue
- eLife
- Publisher
- eLife Sciences Publications, Ltd
- Published
- 2019-09-19
- Volume
- 8
- ISSN
- 2050-084X (ISSN-L)
- OpenAlex
- W2973298828
- Cited by
- 300
- References
- 5
- FWCI
- 11.75
- Type
- journal article
- Language
- en
- Open access
- gold
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