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Cited by 65Microtubule and mitosis dynamicsCancer Treatment and PharmacologySynthesis and biological activity

Structural model for differential cap maturation at growing microtubule ends

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Estévez-Gallego, Juan, Josa-Prado, Fernando, Ku, Siou, Buey, Ruben M, Balaguer, Francisco A, Prota, Andrea E, Lucena-Agell, Daniel, Kamma-Lorger, Christina +9 more·2020·eLife
DOI 10.7554/elife.50155PMC PMC7064335
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Abstract

Microtubules (MTs) are hollow cylinders made of tubulin, a GTPase responsible for essential functions during cell growth and division, and thus, key target for anti-tumor drugs. In MTs, GTP hydrolysis triggers structural changes in the lattice, which are responsible for interaction with regulatory factors. The stabilizing GTP-cap is a hallmark of MTs and the mechanism of the chemical-structural link between the GTP hydrolysis site and the MT lattice is a matter of debate. We have analyzed the structure of tubulin and MTs assembled in the presence of fluoride salts that mimic the GTP-bound and GDP•Pi transition states. Our results challenge current models because tubulin does not change axial length upon GTP hydrolysis. Moreover, analysis of the structure of MTs assembled in the presence of several nucleotide analogues and of taxol allows us to propose that previously described lattice expansion could be a post-hydrolysis stage involved in Pi release.

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  • In vitro, dynamic microtubules have predominantly compacted lattice spacing, while Taxol- and GMPCPP-bound microtubules have expanded lattice spacing.

    “Our analysis recovered the expected lattice spacings, namely predominantly compacted for dynamic microtubules, and expanded for Taxol- and GMPCPP-bound microtubules, verifying our approach (Fig. 1 E).”

    From StableMARK-decorated microtubules in cells have expanded lattices (2025) · cites this paper as Estevez-Gallego et al., 2020

  • The Taxol-induced expansion observed in vitro (41.2-42.0 Å) is considerably smaller than the average hyper-expansion of 42.6 Å measured in situ.

    “Remarkably, the Taxol-induced expansion to 41.2–42.0 Å observed in vitro (Alushin et al., 2014; Estevez-Gallego et al., 2020; Kellogg et al., 2017; Rai et al., 2020; Vale et al., 1994) is considerably smaller than the average hyper-expansion of 42.6 Å measured using our in situ approach.”

    From StableMARK-decorated microtubules in cells have expanded lattices (2025) · cites this paper as Estevez-Gallego et al., 2020

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Authors
Estévez-Gallego, J., Josa-Prado, F., Ku, S., Buey, R. M., Balaguer, F. A., Prota, A. E., Lucena-Agell, D., Kamma-Lorger, C., Yagi, T., Iwamoto, H., Duchesne, L., Barasoain, I., Steinmetz, M. O., Chrétien, D., Kamimura, S., Díaz, J. F., & Oliva, M. A.
Affiliations
  • Structural and Chemical Biology Department, Centro de Investigaciones Biológicas, CSIC, Madrid, Spain
  • Centre National de la Recherche Scientifique (FR)
  • Paul Scherrer Institute (CH)
  • ALBA synchrotron, CELLS, Cerdanyola del Vallès, Spain
  • Prefectural University of Hiroshima (JP)
  • Japan Synchrotron Radiation Research Institute (JP)
  • University of Basel (CH)
  • Chuo University (JP)
  • Consejo Superior de Investigaciones Científicas (ES)
ORCID
Venue
eLife
Publisher
eLife Sciences Publications, Ltd
Published
2020-03-10
Volume
9
Pages
e50155
ISSN
2050-084X (ISSN-L)
DOI
10.7554/elife.50155
PMC
PMC7064335
OpenAlex
W3006609370
Cited by
65
References
1
Citations by year
2026: 14 · 2025: 7 · 2024: 7 · 2023: 11 · 2022: 11 · 2021: 7 · 2020: 8
FWCI
4.57
Type
journal article
Language
en
Open access
Licence
Locations
  • eLife (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Gredos (University of Salamanca) (publishedVersion, cc-by-nc-nd)
  • Hispana (publishedVersion, cc-by-nc-nd)
  • DORA PSI (Paul Scherrer Institute) (submittedVersion, cc-by)
  • HAL (submittedVersion, cc-by)
  • DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Ministerio de Economía y Competitividad (BFU2013-47014P, RYC-2011-07900, BFU2016-75319-R)
  • H2020 European Research Council (H2020-MSCA-ITN-EJD-860070)
  • Swiss National Science Foundation (31003A_166608)
  • Japan Society for the Promotion of Science (KAKENHI 16K07328/17H03668)
  • Agence Nationale de la Recherche (ANR-16-C11-0017-01)
  • SPring-8 Proposal (2016B1182, 2019B1365)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Health Sciences › Medicine › Oncology › Cancer Treatment and Pharmacology
  • Physical Sciences › Chemistry › Organic Chemistry › Synthesis and biological activity
Keywords
tubulin, GTP hydrolysis, microtubule lattice, fluoride salts, nucleotide analogues, paclitaxel, lattice expansion, phosphate release, microtubule growth
MeSH terms
Guanosine Diphosphate, Guanosine Triphosphate, Hydrogen Bonding, Microtubules, Models, Molecular, Molecular Conformation, Structure-Activity Relationship, Tubulin, Cryoelectron Microscopy

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