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Cited by 33Cellular transport and secretionAutophagy in Disease and TherapyCaveolin-1 and cellular processes

Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors

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Wang, Dawei, Ye, Zuodong, Wei, Wenjie, Yu, Jingting, Huang, Lihong, Zhang, Hongmin, Yue, Jianbo·2021·eLife
DOI 10.7554/elife.65910PMC PMC8654373
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Abstract

Actin filaments (F-actin) have been implicated in various steps of endosomal trafficking, and the length of F-actin is controlled by actin capping proteins, such as CapZ, which is a stable heterodimeric protein complex consisting of α and β subunits. However, the role of these capping proteins in endosomal trafficking remains elusive. Here, we found that CapZ docks to endocytic vesicles via its C-terminal actin-binding motif. CapZ knockout significantly increases the F-actin density around immature early endosomes, and this impedes fusion between these vesicles, manifested by the accumulation of small endocytic vesicles in CapZ-knockout cells. CapZ also recruits several RAB5 effectors, such as Rabaptin-5 and Rabex-5, to RAB5-positive early endosomes via its N-terminal domain, and this further activates RAB5. Collectively, our results indicate that CapZ regulates endosomal trafficking by controlling actin density around early endosomes and recruiting RAB5 effectors.

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  • CapZ family proteins are important for endosome maturation.

    “which are important for endosome maturation”

    From INPP5D/SHIP1 regulates endo-lysosomal function and cargo-selective phagocytosis in human microglia (2025) · cites this paper as [24]

    The Results and Discussion report that CapZ is required for early endosome maturation: CapZβ-knockout cells had smaller, more numerous early endosomes, and restoring CapZβ reversed the phenotype. The paper supports the claim for CapZ, but these passages do not establish that CapZ family proteins generally have this role. (LLM, conf=0.79)

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Cited by

Sorted by citations

Authors
Wang, D., Ye, Z., Wei, W., Yu, J., Huang, L., Zhang, H., & Yue, J.
Affiliations
  • City University of Hong Kong (HK)
  • Southern University of Science and Technology (CN)
  • City University of Hong Kong, Shenzhen Research Institute (CN)
Corresponding author
Yue, Jianbo
ORCID
Venue
eLife
Publisher
eLife Sciences Publications, Ltd
Published
2021-11-19
Volume
10
ISSN
2050-084X (ISSN-L)
DOI
10.7554/elife.65910
PMC
PMC8654373
OpenAlex
W3213535530
Cited by
33
References
1
Citations by year
2026: 6 · 2025: 12 · 2024: 9 · 2023: 4 · 2022: 2
FWCI
1.96
Type
journal article
Language
en
Open access
Licence
Locations
  • eLife (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • CityU Scholars (submittedVersion, cc-by)
Funders
  • Research Grants Council, University Grants Committee (11101717, 11103620)
  • National Natural Science Foundation of China (21778045, 2070702)
  • Science, Technology and Innovation Commission of Shenzhen Municipality (JCYJ20160229165235739, JCYJ20170413141331470)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Caveolin-1 and cellular processes
Keywords
CapZ, actin capping protein, endosomal trafficking, early endosomes, endocytic vesicles, Rab5, Rabaptin-5, Rabex-5, vesicle fusion, endocytosis
MeSH terms
Actins, Humans, Endosomes, rab5 GTP-Binding Proteins, Transport Vesicles, CapZ Actin Capping Protein

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