Identification of the Switch in Early-to-Late Endosome Transition
Open accessAbstract
Sequential transport from early to late endosomes requires the coordinated activities of the small GTPases Rab5 and Rab7. The transition between early and late endosomes could be mediated either through transport carriers or by Rab conversion, a process in which the loss of Rab5 from an endosome occurs concomitantly to the acquisition of Rab7. We demonstrate that Rab conversion is the mechanism by which proteins pass from early to late endosomes in Caenorhabditis elegans coelomocytes. Moreover, we identified SAND-1/Mon1 as the critical switch for Rab conversion in metazoa. SAND-1 serves a dual role in this process. First, it interrupts the positive feedback loop of RAB-5 activation by displacing RABX-5 from endosomal membranes; second, it times the recruitment of RAB-7, probably through interaction with the HOPS complex to the same membranes. SAND-1/Mon1 thus acts as a switch by controlling the localization of RAB-5 and RAB-7 GEFs.
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Internalized cargo localizes to late endosomes approximately 10 minutes after uptake.
“previous studies established that internalized cargo localizes to late endosomes ~10 minutes after uptake29–31”
From INPP5D/SHIP1 regulates endo-lysosomal function and cargo-selective phagocytosis in human microglia (2025) · cites this paper as [31]
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- Authors
- Poteryaev, D., Datta, S., Ackema, K., Zerial, M., & Spang, A.
- Affiliations
- University of Basel (CH)
- Max Planck Institute of Molecular Cell Biology and Genetics (DE)
- Corresponding author
- Spang, Anne
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2010-04
- Volume
- 141
- Issue
- 3
- Pages
- 497-508
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2168210128
- Cited by
- 752
- References
- 1
- Citations by year
- 2026: 27 · 2025: 29 · 2024: 54 · 2023: 57 · 2022: 63 · 2021: 66 · 2020: 42 · 2019: 44 · 2018: 42 · 2017: 40 · 2016: 45 · 2015: 49 · 2014: 46 · 2013: 50 · 2012: 48
- FWCI
- 20.69
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Max Planck Institute for Plasma Physics (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Calcium signaling and nucleotide metabolism
- Keywords
- Rab5, Rab7, HOPS complex, endosomal transport, positive feedback loop
- MeSH terms
- rab7 GTP-Binding Proteins, Animals, Phosphatidylinositols, Endosomes, Caenorhabditis elegans, Guanine Nucleotide Exchange Factors, rab GTP-Binding Proteins, rab5 GTP-Binding Proteins, Caenorhabditis elegans Proteins, Animals, Genetically Modified
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