Time-resolved single-cell sequencing identifies multiple waves of mRNA decay during the mitosis-to-G1 phase transition
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Abstract
Accurate control of the cell cycle is critical for development and tissue homeostasis, and requires precisely timed expression of many genes. Cell cycle gene expression is regulated through transcriptional and translational control, as well as through regulated protein degradation. Here, we show that widespread and temporally controlled mRNA decay acts as an additional mechanism for gene expression regulation during the cell cycle in human cells. We find that two waves of mRNA decay occur sequentially during the mitosis-to-G1 phase transition, and we identify the deadenylase CNOT1 as a factor that contributes to mRNA decay during this cell cycle transition. Collectively, our data show that, akin to protein degradation, scheduled mRNA decay helps to reshape cell cycle gene expression as cells move from mitosis into G1 phase.
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Cites
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- Authors
- Krenning, L., Sonneveld, S., & Tanenbaum, M. E.
- Affiliations
- The Netherlands Cancer Institute (NL)
- University Medical Center Utrecht (NL)
- Corresponding author
- Tanenbaum, Marvin E
- Venue
- eLife
- Publisher
- eLife Sciences Publications, Ltd
- Published
- 2022-02-01
- Volume
- 11
- ISSN
- 2050-084X (ISSN-L)
- PMC
- PMC8806192
- OpenAlex
- W4210361290
- Cited by
- 41
- References
- 6
- Citations by year
- 2026: 7 · 2025: 11 · 2024: 8 · 2023: 6 · 2022: 6 · 2021: 3
- FWCI
- 2.53
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- eLife (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Utrecht University Repository (Utrecht University) (submittedVersion, other-oa)
- Funders
- European Research Council (ERCSTG 677936-RNAREG)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- mRNA degradation, cell cycle, CNOT1, deadenylase, gene expression regulation, post-transcriptional regulation, human cells
- MeSH terms
- Cell Cycle, Cell Line, Gene Expression Regulation, Humans, Transcription Factors, Sequence Analysis, RNA, RNA Stability, HEK293 Cells
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