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Cited by 10Biochemical and Structural CharacterizationBacterial Genetics and BiotechnologyEscherichia coli research studies

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Structural foundation for the role of enterococcal PrgB in conjugation, biofilm formation, and virulence

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Sun, Wei-Sheng, Lassinantti, Lena, Järvå, Michael, Schmitt, Andreas, ter Beek, Josy, Berntsson, Ronnie P-A·2023·eLife
DOI 10.7554/elife.84427.3

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Abstract

Type 4 Secretion Systems are a main driver for the spread of antibiotic resistance genes and virulence factors in bacteria. In Gram-positives, these secretion systems often rely on surface adhesins to enhance cellular aggregation and mating-pair formation. One of the best studied adhesins is PrgB from the conjugative plasmid pCF10 of Enterococcus faecalis , which has been shown to play major roles in conjugation, biofilm formation, and importantly also in bacterial virulence. Since prgB orthologs exist on a large number of conjugative plasmids in various different species, this makes PrgB a model protein for this widespread virulence factor. After characterizing the polymer adhesin domain of PrgB previously, we here report the structure for almost the entire remainder of PrgB, which reveals that PrgB contains four immunoglobulin (Ig)-like domains. Based on this new insight, we re-evaluate previously studied variants and present new in vivo data where specific domains or conserved residues have been removed. For the first time, we can show a decoupling of cellular aggregation from biofilm formation and conjugation in prgB mutant phenotypes. Based on the presented data, we propose a new functional model to explain how PrgB mediates its different functions. We hypothesize that the Ig-like domains act as a rigid stalk that presents the polymer adhesin domain at the right distance from the cell wall.

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Metadata

Authors
Sun, W.-S., Lassinantti, L., Järvå, M., Schmitt, A., ter Beek, J., & Berntsson, R. P.-A.
Affiliations
Umeå University (SE)
ORCID
Venue
eLife
Publisher
eLife Sciences Publications, Ltd
Published
2023-10-20
Volume
12
ISSN
2050-084X (ISSN-L)
DOI
10.7554/elife.84427.3
OpenAlex
W4387815499
Cited by
10
References
1
Citations by year
2025: 5 · 2024: 5
FWCI
0.89
Type
journal article
Language
en
Open access
Licence
Locations
eLife (publishedVersion, cc-by)
Funders
  • Vetenskapsrådet (2016-03599)
  • Kempestiftelserna (SMK-1762, SMK-1869)
  • Carl Tryggers Stiftelse för Vetenskaplig Forskning (CTS 18:39)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
Keywords
Enterococcus faecalis, conjugation, biofilm formation, bacterial virulence, type IV secretion system, immunoglobulin-like domains, protein structure, surface adhesins, mating pair formation, cell aggregation

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