Effect of Some Fermentation Substrates and Growth Temperature on Exopolysaccharide Production by Streptococcus thermophilus BN1
Abstract
The purpose of this research was to evaluate, the effect of incubation temperature and some substrates on exopolysaccharides (EPS) production. The S. thermophilus BN1 an EPS-producer strain isolated from cow milk was examined in this study. Skimmed milk, whole milk and cheese whey, were selected as culture media and two different temperatures were also tested. The EPS produced in the different studied conditions was purified and quantified. The strain BN1 was able to produce EPS in all established conditions. However, significant differences were observed in the amounts of produced EPS. The strain has been shown to produce high amounts of EPS at 37°C compared to 42°C (P<0.05). In skimmed milk at 37°C, the EPS amounts reached 548 PDM mg l-1, followed by 375 PDM mg l-1 and 325 PDM mg l-1 respectively in whey and whole milk. In addition, a slight significant difference was noted on the biomass, pH and lactic acid values obtained from the three fermented substrates (p <0.05). The present results demonstrate that suboptimal growth temperature (37°C) had a significant effect on the EPS production by S. thermophilus BN1.
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S. thermophilus exhibits high EPS production in milk medium compared to MRS or M17 broths.
“Additionally, S. thermophilus has been found to exhibit high EPS production in milk medium in comparison to MRS or M17 broths [59].”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [59]
Sub-optimal temperature is the optimal temperature for higher EPS production.
“In turn, several studies reported that sub-optimal temperature is the optimal temperature for higher EPS production [59,67]”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [59]
S. thermophilus BN1 showed an EPS production in skim milk 5-fold higher at 37 °C compared to 42 °C.
“S. thermophilus BN1 showed an EPS production in skim milk 5-fold higher at 37 °C compared to that of 42 °C [59].”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [59]
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- Authors
- Rabha, B., Nadra, R.-S., & Ahmed, B.
- Venue
- International Journal of Bioscience, Biochemistry and Bioinformatics
- Publisher
- IACSIT Press
- Published
- 2012
- Pages
- 44-47
- ISSN
- 2010-3638 (ISSN-L)
- OpenAlex
- W2313591957
- Cited by
- 24
- References
- 20
- Citations by year
- 2025: 1 · 2024: 2 · 2023: 3 · 2022: 1 · 2021: 3 · 2020: 2 · 2019: 3 · 2018: 3 · 2017: 1 · 2016: 1 · 2015: 1 · 2014: 1 · 2013: 2
- FWCI
- 1.97
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- International Journal of Bioscience Biochemistry and Bioinformatics (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biopolymer Synthesis and Applications
- Keywords
- exopolysaccharide production, Streptococcus thermophilus, growth temperature, fermentation substrate, skim milk, whole milk, whey, lactic acid bacteria, dairy fermentation, lactic acid, biomass, pH
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