Glass, John I.

Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome

Gibson, D. G., Glass, J. I., Lartigue, C., Noskov, V. N., Chuang, R.-Y., Algire, M. A., Benders, G. A., Montague, M. G., Ma, L., Moodie, M. M., et al. (2010) · Science

Microbial Metabolic Engineering and BioproductionRNA and protein synthesis mechanisms

Cited by 2,638

Design and synthesis of a minimal bacterial genome

Hutchison, C. A., Chuang, R.-Y., Noskov, V. N., Assad-Garcia, N., Deerinck, T. J., Ellisman, M. H., Gill, J., Kannan, K., Karas, B. J., Ma, L., et al. (2016) · Science

Microbial Metabolic Engineering and BioproductionRNA and protein synthesis mechanisms

Cited by 1,500

A Whole-Cell Computational Model Predicts Phenotype from Genotype

Karr, J. R., Sanghvi, J. C., Macklin, D. N., Gutschow, M. V., Jacobs, J. M., Bolival, B., Assad-Garcia, N., Glass, J. I., & Covert, M. W. (2012) · Cell

Gene Regulatory Network AnalysisMicrobial Metabolic Engineering and Bioproduction

Cited by 1,300

Complete Chemical Synthesis, Assembly, and Cloning of a Mycoplasma genitalium Genome

Gibson, D. G., Benders, G. A., Andrews-Pfannkoch, C., Denisova, E. A., Baden-Tillson, H., Zaveri, J., Stockwell, T. B., Brownley, A., Thomas, D. W., Algire, M. A., et al. (2008) · Science

CRISPR and Genetic EngineeringMicrobial infections and disease research

Cited by 1,243

Essential genes of a minimal bacterium

Glass, J. I., Assad-Garcia, N., Alperovich, N., Yooseph, S., Lewis, M. R., Maruf, M., Hutchison, C. A., Smith, H. O., & Venter, J. C. (2006) · Proceedings of the National Academy of Sciences

Bacterial Genetics and BiotechnologyMicrobial infections and disease research

Cited by 912

Creating Bacterial Strains from Genomes That Have Been Cloned and Engineered in Yeast

Lartigue, C., Vashee, S., Algire, M. A., Chuang, R.-Y., Benders, G. A., Ma, L., Noskov, V. N., Denisova, E. A., Gibson, D. G., Assad-Garcia, N., et al. (2009) · Science

CRISPR and Genetic EngineeringMicrobial Metabolic Engineering and Bioproduction

Cited by 332

Essential metabolism for a minimal cell

Breuer, M., Earnest, E. E., Merryman, C., Wise, K. S., Sun, L., Lynott, M. R., Hutchison, C. A., Smith, H. O., Lapek, J. D., Gonzalez, D. J., et al. (2019) · eLife

Microbial Metabolic Engineering and BioproductionGene Regulatory Network Analysis

Cited by 190

Fundamental behaviors emerge from simulations of a living minimal cell

Thornburg, Z. R., Bianchi, D. M., Brier, T. A., Gilbert, B. R., Earnest, E. E., Melo, M. C. R., Safronova, N., Sáenz, J. P., Cook, A. T., Wise, K. S., et al. (2022) · Cell

Lipid Membrane Structure and BehaviorLipid metabolism and biosynthesis

Cited by 165

Genetic requirements for cell division in a genomically minimal cell

Pelletier, J. F., Sun, L., Wise, K. S., Assad-Garcia, N., Karas, B. J., Deerinck, T. J., Ellisman, M. H., Mershin, A., Gershenfeld, N., Chuang, R.-Y., et al. (2020) · bioRxiv / Cell

Bacterial Genetics and BiotechnologyMicrobial Metabolic Engineering and Bioproduction

Cited by 148

A genome-scale metabolic reconstruction of Mycoplasma genitalium, iPS189

Suthers, P. F., Dasika, M. S., Kumar, V. S., Denisov, G., Glass, J. I., & Maranas, C. D. (2009) · PLoS Computational Biology

Microbial Metabolic Engineering and BioproductionGene Regulatory Network Analysis

Cited by 144

Confronting risks of mirror life

Adamala, K. P., Agashe, D., Belkaid, Y., Bittencourt, D. M. D. C., Cai, Y., Chang, M. W., Chen, I. A., Church, G. M., Cooper, V. S., Davis, M. M., et al. (2024) · Science

Space Science and Extraterrestrial LifeOrigins and Evolution of Life

Cited by 125

Evolution of a minimal cell

Moger-Reischer, R. Z., Glass, J. I., Wise, K. S., Sun, L., Bittencourt, D. M. C., Lehmkuhl, B. K., Schoolmaster, D. R., Lynch, M., & Lennon, J. T. (2023) · Nature

Evolution and Genetic DynamicsMicrobial Metabolic Engineering and Bioproduction

Cited by 116

Bringing the genetically minimal cell to life on a computer in 4D

Thornburg, Z. R., Maytin, A., Kwon, J., Brier, T. A., Gilbert, B. R., Fu, E., Gao, Y.-L., Quenneville, J., Wu, T., Li, H., et al. (2026) · Cell

Gene Regulatory Network AnalysisRNA and protein synthesis mechanisms

Cited by 44

Bringing the Genetically Minimal Cell to Life on a Computer in 4D

Thornburg, Z. R., Maytin, A., Kwon, J., Brier, T. A., Gilbert, B. R., Fu, E., Gao, Y.-L., Quenneville, J., Wu, T., Li, H., et al. (2025) · Cell

Gene Regulatory Network AnalysisRNA and protein synthesis mechanisms

Cited by 44

‘Generating chromosome geometries in a minimal cell from cryo-electron tomograms and chromosome conformation capture maps’

Gilbert, B. R., Thornburg, Z. R., Lam, V., Rashid, F.-Z. M., Glass, J. I., Villa, E., Dame, R. T., & Luthey-Schulten, Z. (2021) · Frontiers in Molecular Biosciences

Genomics and Chromatin DynamicsRNA and protein synthesis mechanisms

Cited by 30

Metabolite Damage and Damage-Control in a Minimal Genome

Haas, D., Thamm, A. M., Sun, J., Huang, L., Sun, L., Beaudoin, G. A. W., Wise, K. S., Lerma-Ortiz, C., Bruner, S. D., Breuer, M., et al. (2021) · mBio

Microbial Metabolic Engineering and BioproductionBacterial Genetics and Biotechnology

Cited by 24

Adaptive evolution of a minimal organism with a synthetic genome

Sandberg, T. E., Wise, K. S., Dalldorf, C., Szubin, R., Feist, A. M., Glass, J. I., & Palsson, B. O. (2023) · iScience

Evolution and Genetic DynamicsMicrobial Metabolic Engineering and Bioproduction

Cited by 23

Kinetic modeling of the genetic information processes in a minimal cell

Thornburg, Z. R., Melo, M. C. R., Bianchi, D., Brier, T. A., Crotty, C., Breuer, M., Smith, H. O., Hutchison, C. A., Glass, J. I., & Luthey-Schulten, Z. (2019) · Frontiers in Molecular Biosciences

Microbial Metabolic Engineering and BioproductionGene Regulatory Network Analysis

Cited by 22

Toward the Complete Functional Characterization of a Minimal Bacterial Proteome

Bianchi, D. M., Pelletier, J. F., Hutchison, C. A., Glass, J. I., & Luthey-Schulten, Z. (2022) · The Journal of Physical Chemistry B

Microbial Metabolic Engineering and BioproductionProtein Structure and Dynamics

Cited by 22