Complete Chemical Synthesis, Assembly, and Cloning of a Mycoplasma genitalium Genome

The Minimal Gene Complement of <i>Mycoplasma genitalium</i>

Fraser, C. M., Gocayne, J. D., White, O., Adams, M. D., Clayton, R. A., Fleischmann, R. D., Bult, C. J., Kerlavage, A. R., Sutton, G., Kelley, J. M., et al. (1995) · Science · doi:10.1126/science.270.5235.397 · Cited by 2,545 · see citing works

Cloning of Large Segments of Exogenous DNA into Yeast by Means of Artificial Chromosome Vectors

Burke, D., Carle, G. F., & Olson, M. V. (1987) · Science · doi:10.1126/science.3033825 · Cited by 1,511 · see citing works

Efficient Initiation of HCV RNA Replication in Cell Culture

Blight, K. J., Kolykhalov, A. A., & Rice, C. M. (2000) · Science · doi:10.1126/science.290.5498.1972 · Cited by 1,420 · see citing works

Chemical synthesis of poliovirus cDNA: generation of infectious virus in the absence of natural template.

Cello, J., Paul, A. V., & Wimmer, E. (2002) · Science · doi:10.1126/science.1072266 · Cited by 980 · see citing works

Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC

Li, M. Z., & Elledge, S. J. (2007) · Nature Methods · doi:10.1038/nmeth1010 · Cited by 939 · see citing works

Global Transposon Mutagenesis and a Minimal Mycoplasma Genome

Hutchison, C. A., Peterson, S. N., Gill, S. R., Cline, R. T., White, O. R., Fraser, C. M., Smith, H. O., & Venter, J. C. (1999) · Science · doi:10.1126/science.286.5447.2165 · Cited by 925 · see citing works

Essential genes of a minimal bacterium

Glass, J. I., Assad-Garcia, N., Alperovich, N., Yooseph, S., Lewis, M. R., Maruf, M., Hutchison, C. A., Smith, H. O., & Venter, J. C. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0510013103 · Cited by 912 · see citing works

Generating a synthetic genome by whole genome assembly: φX174 bacteriophage from synthetic oligonucleotides

Smith, H. O., Hutchison, C. A., Pfannkoch, C. M., & Venter, J. C. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2237126100 · Cited by 612 · see citing works

Genome Transplantation in Bacteria: Changing One Species to Another

Lartigue, C., Glass, J. I., Alperovich, N., Pieper, R., Parmar, P. P., Hutchison, C. A., Smith, H. O., & Venter, J. C. (2007) · Science · doi:10.1126/science.1144622 · Cited by 485 · see citing works

Idempotent Vector Design for Standard Assembly of Biobricks

Knight, T. (2003) · doi:10.21236/ada457791 · Cited by 397 · see citing works

Systematic Assembly of a Full-Length Infectious cDNA of Mouse Hepatitis Virus Strain A59

Yount, B. L., Denison, M. R., Weiss, S. R., & Baric, R. S. (2002) · Journal of Virology · doi:10.1128/jvi.76.21.11065-11078.2002 · Cited by 324 · see citing works

The structure and function of yeast ARS elements

Newlon, C. S., & Theis, J. F. (1993) · Current Opinion in Genetics & Development · doi:10.1016/s0959-437x(05)80094-2 · Cited by 250 · see citing works

Total synthesis of long DNA sequences: Synthesis of a contiguous 32-kb polyketide synthase gene cluster

Kodumal, S. J., Patel, K. G., Reid, R. C., Menzella, H. G., Welch, M., & Santi, D. V. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0406911101 · Cited by 237 · see citing works

Combining two genomes in one cell: Stable cloning of the Synechocystis PCC6803 genome in the Bacillus subtilis 168 genome

Itaya, M., Tsuge, K., Koizumi, M., et al. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0503868102 · Cited by 191 · see citing works

Transformation of Escherichia coli with large DNA molecules by electroporation

Sheng, Y., Mancino, V., & Birren, B. W. (1995) · Nucleic Acids Research · doi:10.1093/nar/23.11.1990 · Cited by 191 · see citing works

Bottom-up genome assembly using the Bacillus subtilis genome vector

Itaya, M., Fujita, K., Kuroki, A., et al. (2007) · Nature Methods · doi:10.1038/nmeth1143 · Cited by 151 · see citing works

DNA-based watermarks using the DNA-Crypt algorithm

Heider, D., & Barnekow, A. (2007) · BMC Bioinformatics · doi:10.1186/1471-2105-8-176 · Cited by 146 · see citing works

Peptide Methionine Sulfoxide Reductase (MsrA) Is a Virulence Determinant in Mycoplasma genitalium

Dhandayuthapani, S., Blaylock, M. W., Bébéar, C., Rasmussen, W. G., & Baseman, J. B. (2001) · Journal of Bacteriology · doi:10.1128/jb.183.19.5645-5650.2001 · Cited by 122 · see citing works

Direct isolation of human BRCA2 gene by transformation-associated recombination in yeast

Larionov, V., Kouprina, N., Solomon, G. G., et al. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.14.7384 · Cited by 95 · see citing works

Secret Signatures Inside Genomic DNA

Arita, M., & Ohashi, Y. (2004) · Biotechnology Progress · doi:10.1021/bp049917i · Cited by 93 · see citing works

Transfer of YACs up to 2.3 Mb intact into human cells with polyethylenimine

Marschall, P., Malik, N., & Larin, Z. (1999) · Gene Therapy · doi:10.1038/sj.gt.3300975 · Cited by 85 · see citing works

A physical map of the Mycoplasma genitalium genome

Colman, S. D., Hu, P., Litaker, W., et al. (1990) · Molecular Microbiology · doi:10.1111/j.1365-2958.1990.tb00638.x · Cited by 61 · see citing works

Highly selective isolation of human DNAs from rodent–human hybrid cells as circular yeast artificial chromosomes by transformation-associated recombination cloning

Larionov, V., Kouprina, N., Graves, J. P., et al. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.24.13925 · Cited by 59 · see citing works

Defining the minimal length of sequence homology required for selective gene isolation by TAR cloning

Noskov, V. N. (2001) · Nucleic Acids Research · doi:10.1093/nar/29.6.e32 · Cited by 57 · see citing works

Large-Insert BAC/YAC Libraries for Selective Re-isolation of Genomic Regions by Homologous Recombination in Yeast

Zeng, C., Kouprina, N., Zhu, B., Cairo, A., Hoek, M., Cross, G., Osoegawa, K., Larionov, V., & de Jong, P. (2001) · Genomics · doi:10.1006/geno.2001.6616 · Cited by 50 · see citing works

Segments missing from the draft human genome sequence can be isolated by transformation‐associated recombination cloning in yeast

Kouprina, N., Leem, S., Solomon, G. G., et al. (2003) · EMBO Reports · doi:10.1038/sj.embor.embor766 · Cited by 41 · see citing works

Optimum conditions for selective isolation of genes from complex genomes by transformation-associated recombination cloning

Leem, S. (2003) · Nucleic Acids Research · doi:10.1093/nar/gng029 · Cited by 40 · see citing works

Rebuilding microbial genomes

Holt, R. A., Warren, R. M., Flibotte, S., Missirlis, P. I., & Smailus, D. E. (2007) · BioEssays · doi:10.1002/bies.20585 · Cited by 22 · see citing works

Selective Isolation of Large Chromosomal Regions by Transformation-Associated Recombination Cloning for Structural and Functional Analysis of Mammalian Genomes

Kouprina, N., Noskov, V. N., & Larionov, V. (2006) · Humana Press eBooks · doi:10.1385/1-59745-158-4:85 · Cited by 18 · see citing works

Purification of YAC-Containing Total Yeast DNA

Silverman, G. A. (2003) · Humana Press eBooks · doi:10.1385/0-89603-313-9:65 · Cited by 9 · see citing works

Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome

Gibson, D. G., Glass, J. I., Lartigue, C., Noskov, V. N., Chuang, R.-Y., Algire, M. A., Benders, G. A., Montague, M. G., Ma, L., Moodie, M. M., et al. (2010) · Science · doi:10.1126/science.1190719 · Cited by 2,638 · see citing works

Automated design of synthetic ribosome binding sites to control protein expression

Salis, H. M., Mirsky, E. A., & Voigt, C. A. (2009) · Nature Biotechnology · doi:10.1038/nbt.1568 · Cited by 1,844 · see citing works

Design and synthesis of a minimal bacterial genome

Hutchison, C. A., Chuang, R.-Y., Noskov, V. N., Assad-Garcia, N., Deerinck, T. J., Ellisman, M. H., Gill, J., Kannan, K., Karas, B. J., Ma, L., et al. (2016) · Science · doi:10.1126/science.aad6253 · Cited by 1,500 · see citing works

Programming cells by multiplex genome engineering and accelerated evolution

Wang, H. H., Isaacs, F. J., Carr, P. A., Sun, Z. Z., Xu, G., Forest, C. R., & Church, G. M. (2009) · Nature · doi:10.1038/nature08187 · Cited by 1,529 · see citing works

A Whole-Cell Computational Model Predicts Phenotype from Genotype

Karr, J. R., Sanghvi, J. C., Macklin, D. N., Gutschow, M. V., Jacobs, J. M., Bolival, B., Assad-Garcia, N., Glass, J. I., & Covert, M. W. (2012) · Cell · doi:10.1016/j.cell.2012.05.044 · Cited by 1,300 · see citing works

The second wave of synthetic biology: from modules to systems

Purnick, P. E. M., & Weiss, R. (2009) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2698 · Cited by 1,181 · see citing works

Proteome organization in a genome-reduced bacterium

Kühner, S., van Noort, V., Betts, M. J., Leo-Macias, A., Batisse, C., Rode, M., Yamada, T., Maier, T., Bader, S., Beltran-Alvarez, P., et al. (2009) · Science · doi:10.1126/science.1176343 · Cited by 471 · see citing works

Synthetic DNA Synthesis and Assembly: Putting the Synthetic in Synthetic Biology

Hughes, R. A., & Ellington, A. D. (2017) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a023812 · Cited by 411 · see citing works

A techno-economic analysis of industrial production of marine microalgae as a source of EPA and DHA-rich raw material for aquafeed: Research challenges and possibilities

Chauton, M. S., Reitan, K. I., Norsker, N. H., Tveterås, R., & Kleivdal, H. T. (2015) · Aquaculture · doi:10.1016/j.aquaculture.2014.10.038 · Cited by 323 · see citing works

Bricks and blueprints: methods and standards for DNA assembly.

Casini, A., Storch, M., Baldwin, G. S., & Ellis, T. (2015) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm4014 · Cited by 286 · see citing works

Recent developments in synthetic biology and metabolic engineering in microalgae towards biofuel production

Jagadevan, S., Banerjee, A., Banerjee, C., Guria, C., Tiwari, R., Baweja, M., & Shukla, P. (2018) · Biotechnology for Biofuels · doi:10.1186/s13068-018-1181-1 · Cited by 275 · see citing works

Realizing the potential of synthetic biology

Church, G. M., Elowitz, M. B., Smolke, C. D., Voigt, C. A., & Weiss, R. (2014) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3767 · Cited by 226 · see citing works

The Anthropocene biosphere

Williams, M., Zalasiewicz, J., Haff, P., Schwägerl, C., Barnosky, A. D., & Ellis, E. C. (2015) · The Anthropocene Review · doi:10.1177/2053019615591020 · Cited by 226 · see citing works

Essential metabolism for a minimal cell

Breuer, M., Earnest, E. E., Merryman, C., Wise, K. S., Sun, L., Lynott, M. R., Hutchison, C. A., Smith, H. O., Lapek, J. D., Gonzalez, D. J., et al. (2019) · eLife · doi:10.7554/elife.36842 · Cited by 190 · see citing works

Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome.

Shen, Y., Wang, Y., Chen, T., Gao, F., Gong, J., Abramczyk, D., Walker, R., Zhao, H., Chen, S., Liu, W., et al. (2017) · Science · doi:10.1126/science.aaf4791 · Cited by 202 · see citing works

The Future of Origin of Life Research: Bridging Decades-Old Divisions

Preiner, M., Asche, S., Becker, S., Betts, H. C., Boniface, A., Camprubi, E., Chandru, K., Erastova, V., Garg, S. G., Khawaja, N., et al. (2020) · Life · doi:10.3390/life10030020 · Cited by 140 · see citing works

Advances and prospects in metabolic engineering of Zymomonas mobilis

Wang, X., He, Q., Yang, Y., Wang, J., Haning, K., Hu, Y., Wu, B., He, M., Zhang, Y., Bao, J., et al. (2018) · Metabolic Engineering · doi:10.1016/j.ymben.2018.04.001 · Cited by 154 · see citing works

A genome-scale metabolic reconstruction of Mycoplasma genitalium, iPS189

Suthers, P. F., Dasika, M. S., Kumar, V. S., Denisov, G., Glass, J. I., & Maranas, C. D. (2009) · PLoS Computational Biology · doi:10.1371/journal.pcbi.1000285 · Cited by 144 · see citing works

A user’s guide to golden gate cloning methods and standards

Bird, J. E., Marles-Wright, J., & Giachino, A. (2022) · ACS Synthetic Biology · doi:10.1021/acssynbio.2c00355 · Cited by 135 · see citing works

Implementation of stable and complex biological systems through recombinase-assisted genome engineering

Santos, C. N. S., Regitsky, D. D., & Yoshikuni, Y. (2013) · Nature Communications · doi:10.1038/ncomms3503 · Cited by 93 · see citing works

Prebiotic Chemistry: What We Know, What We Don’t

Cleaves, H. J. (2012) · Evolution: Education and Outreach · doi:10.1007/s12052-012-0443-9 · Cited by 75 · see citing works

Large-Scale de Novo Oligonucleotide Synthesis for Whole-Genome Synthesis and Data Storage: Challenges and Opportunities

Song, L.-F., Deng, Z.-H., Gong, Z.-Y., Li, L.-L., & Li, B.-Z. (2021) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2021.689797 · Cited by 74 · see citing works

Programming biological operating systems: genome design, assembly and activation.

Gibson, D. G. (2014) · Nature Methods · doi:10.1038/nmeth.2894 · Cited by 65 · see citing works

Systems biology perspectives on minimal and simpler cells

Xavier, J. C., Patil, K. R., & Rocha, I. (2014) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00050-13 · Cited by 58 · see citing works

From Designing the Molecules of Life to Designing Life: Future Applications Derived from Advances in DNA Technologies.

Kohman, R. E., Kunjapur, A. M., Hysolli, E., Wang, Y., & Church, G. M. (2018) · Angewandte Chemie International Edition · doi:10.1002/anie.201707976 · Cited by 33 · see citing works

Toward the Complete Functional Characterization of a Minimal Bacterial Proteome

Bianchi, D. M., Pelletier, J. F., Hutchison, C. A., Glass, J. I., & Luthey-Schulten, Z. (2022) · The Journal of Physical Chemistry B · doi:10.1021/acs.jpcb.2c04188 · Cited by 22 · see citing works

The evolution of <i>Mycoplasma genitalium</i>

Blanchard, A., & Bébéar, C. (2011) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.2011.06418.x · Cited by 22 · see citing works

Application of Array-Based Oligonucleotides for Synthesis of Genetic Designs

Sinyakov, A. N., Ryabinin, V. A., & Kostina, E. V. (2021) · Molecular Biology · doi:10.1134/s0026893321030109 · Cited by 9 · see citing works

Non-random distribution of transposable elements in the nuclear genome of plants

Capel, J., Montero, L. M., Martínez-Zapater, J. M., et al. (1993) · Nucleic Acids Research · doi:10.1093/nar/21.10.2369 · Cited by 27 · see citing works

Transposable elements and plant genome evolution

Sergeeva, E. M., & Salina, E. A. (2011) · Russian Journal of Genetics Applied Research · doi:10.1134/s2079059711060086 · Cited by 9 · see citing works

Active Transposition in Genomes

Huang, C. R. L., Burns, K. H., & Boeke, J. D. (2012) · Annual Review of Genetics · doi:10.1146/annurev-genet-110711-155616 · Cited by 412 · see citing works

Transposons in chloroplast genomes are distributed inhomogeneously among gymnosperms and angiosperms

Mutovina, O., & Sadovsky, M. G. (2020) · Journal of Physics Conference Series · doi:10.1088/1742-6596/1679/3/032095

The Functional Impact of Transposable Elements on the Diversity of Plant Genomes

Grzebelus, D. (2018) · Diversity · doi:10.3390/d10020018 · Cited by 26 · see citing works