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Preparation of Isolated Sheets of Human Stratum Corneum
Abstract
Summary Methods of preparing intact, undenatured sheets of human stratum corneum are described. The epidermis of split or full-thickness skin is first separated by exposure to ammonia fumes or heat at 60 C. The horny layer is then isolated by incubating in dilute trypsin solution. Cantharidin blisters provide a convenient way of obtaining stratum corneum from living subjects with minimum discomfort and no scarring.
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Citations
Cited by
Cross, S. E., Innes, B., Roberts, M. S., Tsuzuki, T., Robertson, T. A., & McCormick, P. (2007) · Skin Pharmacology and Physiology · doi:10.1159/000098701 · Cited by 481
Oestradiol skin delivery from ultradeformable liposomes: refinement of surfactant concentration
El Maghraby, G. M. M., Williams, A. C., & Barry, B. W. (2000) · International Journal of Pharmaceutics · doi:10.1016/s0378-5173(99)00441-x · Cited by 286
Ourique, A. F., Melero, A., Silva, C. D. B. D., Schaefer, U. F., Pohlmann, A. R., Guterres, S. S., Lehr, C.-M., Kostka, K.-H., & Beck, R. C. R. (2011) · European Journal of Pharmaceutics and Biopharmaceutics · doi:10.1016/j.ejpb.2011.03.008 · Cited by 132
Human skin models: From healthy to disease-mimetic systems; characteristics and applications
Moniz, T., Costa Lima, S. A., & Reis, S. (2020) · British Journal of Pharmacology · doi:10.1111/bph.15184 · Cited by 80
Smart polymeric nanofibers for topical delivery of levothyroxine
Fathi Azarbayjani, A., Venugopal, J. R., Ramakrishna, S., Lim, F. C., Chan, Y. W., & Chan, S. Y. (2010) · Journal of Pharmacy & Pharmaceutical Sciences · doi:10.18433/j3ts3g · Cited by 56
Metadata
- Authors
- KLIGMAN, A. M.
- Venue
- Archives of Dermatology
- Publisher
- American Medical Association (AMA)
- Published
- 1963-12-01
- Volume
- 88
- Issue
- 6
- Pages
- 702
- ISSN
- 0003-987X (ISSN-L)
- OpenAlex
- W2141331305
- Cited by
- 775
- References
- 0
- FWCI
- 4.65
- Type
- journal article
- Language
- en
- Open access
- closed
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
