The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Improvement of interferon-γ sialylation in Chinese hamster ovary cell culture by feeding ofN-acetylmannosamine
Abstract
Gas transfer and mixing were characterized in a 32-L bubble column reactor equipped with a commercially available rubber membrane diffuser. The performance of the membrane diffuser indicates that the slits in the membrane are best described as holes with elastic lids, acting as valves cutting off bubbles from the gas stream. The membrane diffuser thus functions as a one-way valve preventing backflow of liquid. Our design of the bottom plate of the reactor enabled us to optimize the aeration by changing the tension of the membrane. We thereby achieved mass transfer coefficients higher than those previously reported in bubble columns. A strong dependence of mass transfer on gas holdup and bubble size was indicated by estimates based on these two variables. The microalga, Rhodomonas sp. , sensitive to chemical and physical stress, was maintained for 8 months in continuous culture with a productivity identical to cultures grown in stirred tank reactors. Copyright 1998 John Wiley & Sons, Inc.
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“Some of these approaches are based on the supplementation of cell culture media with different precursors of the sialic acid pathway, such as CMP-sialic acid, ManNAc and NeuNAc, but their effects are limited and still debated (Gu and Wang, 1998;Baker et al., 2001;Hills et al., 2001;Bork et al., 2009;Hossler et al., 2009;Wong et al., 2010;Kildegaard et al., 2016).”
From Therapeutic glycoprotein production in mammalian cells (2017) · cites this paper as Gu and Wang, 1998
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Metadata
- Authors
- Gu, X., & Wang, D. I. C.
- Venue
- Biotechnology and Bioengineering
- Publisher
- Wiley
- Published
- 1998-06-20
- Volume
- 58
- Issue
- 6
- Pages
- 642-648
- ISSN
- 0006-3592
- 1097-0290
- References
- 28
- Language
- en
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