Adeno‐Associated Viral Vectors for Anterograde Axonal Tracing with Fluorescent Proteins in Nontransgenic and Cre Driver Mice
Abstract
Harnessing the natural ability of viruses to infect post‐mitotic cells such as neurons has provided an explosion of new methods to manipulate and reconstruct neural circuits in vivo. Here we describe the use of recombinant adeno‐associated viral vectors (rAAV) for axonal tract tracing in nontransgenic and transgenic Cre driver mice. Two protocols are presented for stereotactic‐guided placement of rAAV vectors into the live mouse brain using iontophoretic or nanoliter pressure injections. The methods discussed here will result in expression of fluorescent proteins in cell bodies, dendrites, and axons in targeted neurons, and can be easily adapted to address various experimental questions. Curr. Protoc. Neurosci. 59:1.20.1‐1.20.18. © 2012 by John Wiley & Sons, Inc.
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The entire atlas data generation was standardized with the use of AAV serotype 1 and iontophoresis for stereotaxic injections.
“Thus, the entire atlas data generation was standardized with the use of AAV serotype 1 and iontophoresis for stereotaxic injections20.”
From A mesoscale connectome of the mouse brain (2014) · cites this paper as [20]
Cites
A robust and high-throughput Cre reporting and characterization system for the whole mouse brain
Madisen, L., Zwingman, T. A., Sunkin, S. M., Oh, S. W., Zariwala, H. A., Gu, H., Ng, L. L., Palmiter, R. D., Hawrylycz, M. J., Jones, A. R., et al. (2010) · Nature Neuroscience · doi:10.1038/nn.2467 · Cited by 6,907 · see citing works
Self-citationRegulation of parkinsonian motor behaviours by optogenetic control of basal ganglia circuitry
Kravitz, A. V., Freeze, B. S., Parker, P. R. L., Kay, K., Thwin, M. T., Deisseroth, K., & Kreitzer, A. C. (2010) · Nature · doi:10.1038/nature09159 · Cited by 1,885 · see citing works
Adeno-associated Virus Serotypes: Vector Toolkit for Human Gene Therapy
Wu, Z., Asokan, A., & Samulski, R. J. (2006) · Molecular Therapy · doi:10.1016/j.ymthe.2006.05.009 · Cited by 921 · see citing works
Hisano, S., Hoshi, K., Ikeda, Y., Maruyama, D., Kanemoto, M., Ichijo, H., Kojima, I., Takeda, J., & Nogami, H. (2000) · Molecular Brain Research · doi:10.1016/s0169-328x(00)00194-7 · Cited by 806 · see citing works
Dhillon, H., Zigman, J. M., Ye, C., Lee, C. E., McGovern, R. A., Tang, V., Kenny, C. D., Christiansen, L. M., White, R. D., Edelstein, E. A., et al. (2006) · Neuron · doi:10.1016/j.neuron.2005.12.021 · Cited by 791 · see citing works
Burger, C., Gorbatyuk, O. S., Velardo, M. J., Peden, C. S., Williams, P., Zolotukhin, S., Reier, P. J., Mandel, R. J., & Muzyczka, N. (2004) · Molecular Therapy · doi:10.1016/j.ymthe.2004.05.024 · Cited by 741 · see citing works
Serial two-photon tomography for automated ex vivo mouse brain imaging
Ragan, T. M., Kadiri, L. R., Venkataraju, K. U., Bahlmann, K., Sutin, J. D. B., Taranda, J., Arganda‐Carreras, I., Kim, Y., Seung, H. S., & Osten, P. (2012) · Nature Methods · doi:10.1038/nmeth.1854 · Cited by 659 · see citing works
Atasoy, D., Aponte, Y., Su, H. H., & Sternson, S. M. (2008) · Journal of Neuroscience · doi:10.1523/jneurosci.1954-08.2008 · Cited by 614 · see citing works
Spires, T. L., Meyer-Luehmann, M., Stern, E. A., McLean, P. J., Skoch, J., Nguyen, P. T., Bacskai, B. J., & Hyman, B. T. (2005) · The Journal of Neuroscience · doi:10.1523/jneurosci.1879-05.2005 · Cited by 550 · cited 1× · see citing works
“This may be desirable for dynamic or in situ imaging (e.g., Bennett et al., 1997; Spires et al., 2005; Stettler et al., 2006; Madisen et al., 2010; Marik et al., 2010; Mittmann et al., 2011).”
BackgroundComparative Analysis of Adeno-Associated Viral Vector Serotypes 1, 2, 5, 7, And 8 in Mouse Brain
Taymans, J.-M., Vandenberghe, L. H., Haute, C. V. D., Thiry, I., Deroose, C. M., Mortelmans, L., Wilson, J. M., Debyser, Z., & Baekelandt, V. (2007) · Human Gene Therapy · doi:10.1089/hum.2006.178 · Cited by 305 · see citing works
Axons and synaptic boutons are highly dynamic in adult visual cortex
Stettler, D. D., Yamahachi, H., Li, W., Denk, W., & Gilbert, C. D. (2006) · Neuron · doi:10.1016/j.neuron.2006.02.018 · Cited by 256 · see citing works
Chamberlin, N. L., Du, B., de Lacalle, S., & Saper, C. B. (1998) · Brain Research · doi:10.1016/s0006-8993(98)00169-3 · Cited by 221 · see citing works
Passini, M. A., Watson, D. J., Vite, C. H., Landsburg, D. J., Feigenbaum, A. L., & Wolfe, J. H. N. (2003) · Journal of Virology · doi:10.1128/jvi.77.12.7034-7040.2003 · Cited by 213 · see citing works
Cearley, C. N., & Wolfe, J. H. N. (2007) · Journal of Neuroscience · doi:10.1523/jneurosci.2185-07.2007 · Cited by 212 · see citing works
Gene Transfer by Adeno-Associated Virus Vectors into the Central Nervous System
Xiao, X., Li, J., McCown, T. J., & Samulski, R. J. (1997) · Experimental Neurology · doi:10.1006/exnr.1996.6396 · Cited by 202 · see citing works
Real-time, noninvasive in vivo assessment of adeno-associated virus-mediated retinal transduction.
Bennett, J., Duan, D., Engelhardt, J. F., & Maguire, A. M. (1997) · PubMed · Cited by 191 · see citing works
Two-photon calcium imaging of evoked activity from L5 somatosensory neurons in vivo
Mittmann, W., Wallace, D. J., Czubayko, U., Herb, J. T., Schaefer, A. T., Looger, L. L., Denk, W. T., & Kerr, J. N. D. (2011) · Nature Neuroscience · doi:10.1038/nn.2879 · Cited by 188 · see citing works
Expanded Repertoire of AAV Vector Serotypes Mediate Unique Patterns of Transduction in Mouse Brain
Cearley, C. N., Vandenberghe, L. H., Parente, M. K., Carnish, E. R., Wilson, J. M., & Wolfe, J. H. (2008) · Molecular Therapy · doi:10.1038/mt.2008.166 · Cited by 182 · see citing works
Kuhlman, S. J., & Huang, Z. J. (2008) · PLoS ONE · doi:10.1371/journal.pone.0002005 · Cited by 171 · see citing works
Eight Years of Clinical Improvement in MPTP-Lesioned Primates After Gene Therapy With AAV2-hAADC
Hadaczek, P., Eberling, J. L., Pivirotto, P., Bringas, J., Forsayeth, J., & Bankiewicz, K. S. (2010) · Molecular Therapy · doi:10.1038/mt.2010.106 · Cited by 158 · see citing works
Axonal dynamics of excitatory and inhibitory neurons in somatosensory cortex
Marik, S. A., Yamahachi, H., McManus, J. N. J., Szabo, G., & Gilbert, C. D. (2010) · PLoS Biology · doi:10.1371/journal.pbio.1000395 · Cited by 124 · see citing works
The Role of the Adeno-Associated Virus Capsid in Gene Transfer
Vliet, K. V., Blouin, V., Brument, N., Agbandje‐McKenna, M., & Snyder, R. O. (2008) · Methods in molecular biology · doi:10.1007/978-1-59745-210-6_2 · Cited by 113 · see citing works
Adeno-Associated Viral Vectors for Mapping, Monitoring, and Manipulating Neural Circuits
Betley, J. N., & Sternson, S. M. (2011) · Human Gene Therapy · doi:10.1089/hum.2010.204 · Cited by 102 · see citing works
Gautron, L., Lazarus, M., Scott, M. M., Saper, C. B., & Elmquist, J. K. (2010) · The Journal of Comparative Neurology · doi:10.1002/cne.22323 · Cited by 82 · see citing works
Vandenberghe, L. H., Breous, E., Nam, H.-J., Gao, G., Xiao, R., Sandhu, A., Johnston, J., Debyser, Z., Agbandje‐McKenna, M., & Wilson, J. M. (2009) · Gene Therapy · doi:10.1038/gt.2009.101 · Cited by 72 · see citing works
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Tasic, B., Menon, V., Nguyen, T. N., Kim, T. K., Jarsky, T., Yao, Z., Levi, B., Gray, L. T., Sorensen, S. A., Dolbeare, T., et al. (2016) · Nature Neuroscience · doi:10.1038/nn.4216 · Cited by 1,714 · see citing works
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Funk, C. M., Peelman, K., Bellesi, M., Marshall, W., Cirelli, C., & Tononi, G. (2017) · The Journal of Neuroscience · doi:10.1523/jneurosci.1303-17.2017 · Cited by 145 · see citing works
Lindberg, D., Chen, P., & Li, C. (2013) · Journal of Comparative Neurology · doi:10.1002/cne.23338 · Cited by 125 · see citing works
Wang, Q., Henry, A. M., Harris, J. A., Oh, S. W., Joines, K. M., Nyhus, J., Hirokawa, K. E., Dee, N., Mortrud, M., Parry, S., et al. (2014) · Journal of Comparative Neurology · doi:10.1002/cne.23567 · Cited by 44 · see citing works
Self-citation
- Authors
- Harris, J. A., Wook Oh, S., & Zeng, H.
- Affiliations
- Allen Institute for Brain Science (US)
- Allen Institute for Brain Science Seattle Washington
- Venue
- Current Protocols in Neuroscience
- Publisher
- Wiley
- Published
- 2012-04
- Volume
- 59
- Issue
- 1
- Pages
- Unit 1.20.1-18
- ISSN
- 1934-8576 (ISSN-L)
- 1934-8584
- OpenAlex
- W2102015942
- Cited by
- 72
- References
- 25
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2023: 3 · 2022: 7 · 2021: 3 · 2020: 11 · 2019: 5 · 2018: 4 · 2017: 6 · 2016: 6 · 2015: 6 · 2014: 9 · 2013: 9
- FWCI
- 4.61
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Current Protocols in Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Keywords
- adeno-associated virus vectors, anterograde tracing, fluorescent proteins, stereotactic injection, iontophoresis, pressure injection, postmitotic neurons, neural circuit reconstruction
- MeSH terms
- Dependovirus, Animals, Axons, Axonal Transport, Genetic Vectors, Luminescent Proteins, Mice, Transgenic, Neural Pathways, Protein Engineering, Integrases, Mice, Neuroanatomical Tract-Tracing Techniques
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