Cited by 72Virus-based gene therapy researchNeuroscience and Neuropharmacology ResearchRNA Interference and Gene Delivery

Adeno‐Associated Viral Vectors for Anterograde Axonal Tracing with Fluorescent Proteins in Nontransgenic and Cre Driver Mice

Harris, Julie A., Wook Oh, Seung, Zeng, Hongkui·2012·Current Protocols in Neuroscience

Abstract

Harnessing the natural ability of viruses to infect post‐mitotic cells such as neurons has provided an explosion of new methods to manipulate and reconstruct neural circuits in vivo. Here we describe the use of recombinant adeno‐associated viral vectors (rAAV) for axonal tract tracing in nontransgenic and transgenic Cre driver mice. Two protocols are presented for stereotactic‐guided placement of rAAV vectors into the live mouse brain using iontophoretic or nanoliter pressure injections. The methods discussed here will result in expression of fluorescent proteins in cell bodies, dendrites, and axons in targeted neurons, and can be easily adapted to address various experimental questions. Curr. Protoc. Neurosci. 59:1.20.1‐1.20.18. © 2012 by John Wiley & Sons, Inc.

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Crossref · 10.1002/0471142301.ns0120s59

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25 of 25 references verified0 citation errors · 0 not checkable

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  • The entire atlas data generation was standardized with the use of AAV serotype 1 and iontophoresis for stereotaxic injections.

    “Thus, the entire atlas data generation was standardized with the use of AAV serotype 1 and iontophoresis for stereotaxic injections20.”

    From A mesoscale connectome of the mouse brain (2014) · cites this paper as [20]

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Authors
Harris, J. A., Wook Oh, S., & Zeng, H.
Affiliations
  • Allen Institute for Brain Science (US)
  • Allen Institute for Brain Science Seattle Washington
Venue
Current Protocols in Neuroscience
Publisher
Wiley
Published
2012-04
Volume
59
Issue
1
Pages
Unit 1.20.1-18
ISSN
  • 1934-8576 (ISSN-L)
  • 1934-8584
OpenAlex
W2102015942
Cited by
72
References
25
Citations by year
2026: 1 · 2025: 1 · 2024: 1 · 2023: 3 · 2022: 7 · 2021: 3 · 2020: 11 · 2019: 5 · 2018: 4 · 2017: 6 · 2016: 6 · 2015: 6 · 2014: 9 · 2013: 9
FWCI
4.61
Type
journal article
Language
en
Open access
closed
Locations
  • Current Protocols in Neuroscience (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
Keywords
adeno-associated virus vectors, anterograde tracing, fluorescent proteins, stereotactic injection, iontophoresis, pressure injection, postmitotic neurons, neural circuit reconstruction
MeSH terms
Dependovirus, Animals, Axons, Axonal Transport, Genetic Vectors, Luminescent Proteins, Mice, Transgenic, Neural Pathways, Protein Engineering, Integrases, Mice, Neuroanatomical Tract-Tracing Techniques

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