The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Isolation of Whole Mononuclear Cells from Peripheral Blood and Cord Blood
Abstract
Peripheral blood is the primary source of lymphoid cells for investigation of the human immune system. Its use is facilitated by Ficoll‐Hypaque density gradient centrifugation—a simple and rapid method of purifying peripheral blood mononuclear cells (PBMC) that takes advantage of the density differences between mononuclear cells and other elements found in the blood sample. Thus, cells are distributed in the solution in layers based on the differences in their density/size. Additional purification methods can be employed as the mononuclear cell sample can be purified from monocytes by adherence or by exposure to L‐leucine methyl ester; these methods are described for both procedures. Cord blood and peripheral blood from infants contain immature cells, including nucleated red cells, which can result in significant contamination of the mononuclear cell layer, and removal of these cells requires additional steps that are described. The isolation procedures presented here can also be applied to cell populations derived from tissues. Curr. Protoc. Immunol. 85:7.1.1‐7.1.8. © 2009 by John Wiley & Sons, Inc.
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Cites
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The Glomerular Permeability Determined by Dextran Clearance Using Sephadex Gel Filtration
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The mouse excisional wound splinting model, including applications for stem cell transplantation
Wang, X., Ge, J., Tredget, E. E., & Wu, Y. (2013) · Nature Protocols · doi:10.1038/nprot.2013.002 · Cited by 440
Mingozzi, F., Meulenberg, J. J., Hui, D. J., Basner-Tschakarjan, E., Hasbrouck, N. C., Edmonson, S. A., Hutnick, N. A., Betts, M. R., Kastelein, J. J., Stroes, E. S., et al. (2009) · Blood · doi:10.1182/blood-2008-07-167510 · Cited by 295
Fisher, W. E., Cruz-Monserrate, Z., McElhany, A. L., Lesinski, G. B., Hart, P. A., Ghosh, R., Van Buren, G., Fishman, D. S., Rinaudo, J. A. S., Serrano, J., et al. (2018) · Pancreas · doi:10.1097/mpa.0000000000001171 · Cited by 34
Schreurs, R. R. C. E., Drewniak, A., Bakx, R., Corpeleijn, W. E., Geijtenbeek, T. H. B., van Goudoever, J. B., & Bunders, M. J. (2017) · Journal of Immunological Methods · doi:10.1016/j.jim.2017.03.006 · Cited by 25
Metadata
- Authors
- Fuss, I. J., Kanof, M. E., Smith, P. D., & Zola, H.
- Venue
- Current Protocols in Immunology
- Publisher
- Wiley
- Published
- 2009-04
- Volume
- 85
- Issue
- 1
- Pages
- 7.1.1-7.1.8
- ISSN
- 1934-3671 (ISSN-L)
- 1934-368X
- OpenAlex
- W1602010694
- Cited by
- 433
- References
- 6
- FWCI
- 6.62
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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