The DOI matches its Crossref record; 29 of the 30 references it cites were found in a registry or an index.
Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
Abstract
Exosomes are small membrane vesicles found in cell culture supernatants and in different biological fluids. Exosomes form in a particular population of endosomes, called multivesicular bodies (MVBs), by inward budding into the lumen of the compartment. Upon fusion of MVBs with the plasma membrane, these internal vesicles are secreted. Exosomes possess a defined set of membrane and cytosolic proteins. The physiological function of exosomes is still a matter of debate, but increasing results in various experimental systems suggest their involvement in multiple biological processes. Because both cell‐culture supernatants and biological fluids contain different types of lipid membranes, it is critical to perform high‐quality exosome purification. This unit describes different approaches for exosome purification from various sources, and discusses methods to evaluate the purity and homogeneity of the purified exosome preparations.
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“For electron microscopy (EM) analysis of EVs, samples were prepared as per the protocol described previously72.”
From GAPDH controls extracellular vesicle biogenesis and enhances the therapeutic potential of EV mediated siRNA delivery to the brain (2021) · cites this paper as [72]
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Cites
B lymphocytes secrete antigen-presenting vesicles.
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Self-citationIdentification and proteomic profiling of exosomes in human urine
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Tumor-derived exosomes are a source of shared tumor rejection antigens for CTL cross-priming
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Exosomal-like vesicles are present in human blood plasma
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Self-citationCells release prions in association with exosomes
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Self-citationMalignant effusions and immunogenic tumour-derived exosomes
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Self-citationIndirect activation of naïve CD4+ T cells by dendritic cell–derived exosomes
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Self-citationProteomic and Biochemical Analyses of Human B Cell-derived Exosomes
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Intestinal epithelial cells secrete exosome–like vesicles
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Self-citationLipid raft-associated protein sorting in exosomes
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Self-citationTCR Activation of Human T Cells Induces the Production of Exosomes Bearing the TCR/CD3/ζ Complex
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Self-citationICAM-1 on exosomes from mature dendritic cells is critical for efficient naive T-cell priming
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Intestinal epithelial exosomes carry MHC class II/peptides able to inform the immune system in mice
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Research works printed but not linked: 1
- Raposo G., 1997, Handbook of Experimental Immunology, 1
Cited by
Sorted by citations
Théry, C., Witwer, K. W., Aikawa, E., Alcaraz, M. J., Anderson, J. D., Andriantsitohaina, R., Antoniou, A., Arab, T., Archer, F., Atkin‐Smith, G. K., et al. (2018) · Journal of Extracellular Vesicles · doi:10.1080/20013078.2018.1535750 · Cited by 11,099
Self-citationShedding light on the cell biology of extracellular vesicles
van Niel, G., D'Angelo, G., & Raposo, G. (2018) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2017.125 · Cited by 8,369
Self-citationExtracellular vesicles: Exosomes, microvesicles, and friends
Raposo, G., & Stoorvogel, W. (2013) · Journal of Cell Biology · doi:10.1083/jcb.201211138 · Cited by 8,099
Self-citationBiogenesis, Secretion, and Intercellular Interactions of Exosomes and Other Extracellular Vesicles
Colombo, M., Raposo, G., & Théry, C. (2014) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev-cellbio-101512-122326 · Cited by 6,186
Self-citationDelivery of siRNA to the mouse brain by systemic injection of targeted exosomes
Alvarez-Erviti, L., Seow, Y., Yin, H., Betts, C., Lakhal, S., & Wood, M. J. A. (2011) · Nature Biotechnology · doi:10.1038/nbt.1807 · Cited by 4,930
Metadata
- Authors
- Théry, C., Amigorena, S., Raposo, G., & Clayton, A.
- Affiliations
- Institut Curie (FR)
- Institut Curie Paris France
- Cardiff University (GB)
- Venue
- Current Protocols in Cell Biology
- Publisher
- Wiley
- Published
- 2006-03
- Volume
- 30
- Issue
- 1
- Pages
- Unit 3.22-Unit 3.22
- ISSN
- 1934-2500 (ISSN-L)
- 1934-2616
- OpenAlex
- W1877686344
- Cited by
- 5,766
- References
- 30
- Citations by year
- 2026: 206 · 2025: 356 · 2024: 485 · 2023: 502 · 2022: 558 · 2021: 641 · 2020: 620 · 2019: 521 · 2018: 452 · 2017: 390 · 2016: 301 · 2015: 259 · 2014: 177 · 2013: 106 · 2012: 85
- FWCI
- 3.54
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Current Protocols in Cell Biology (publishedVersion)
- PubMed (publishedVersion)
- ORCA Online Research @Cardiff (Cardiff University) (acceptedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Keywords
- exosomes, exosome isolation, multivesicular bodies, cell culture supernatant, biological fluids, exosome characterization, membrane vesicles, exosome function
- MeSH terms
- Animals, Culture Media, Electrophoresis, Polyacrylamide Gel, Flow Cytometry, Humans, Immunohistochemistry, Ultracentrifugation, Cell Culture Techniques, Secretory Vesicles, Microscopy, Electron, Transmission
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