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Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
Abstract
Exosomes are small membrane vesicles found in cell culture supernatants and in different biological fluids. Exosomes form in a particular population of endosomes, called multivesicular bodies (MVBs), by inward budding into the lumen of the compartment. Upon fusion of MVBs with the plasma membrane, these internal vesicles are secreted. Exosomes possess a defined set of membrane and cytosolic proteins. The physiological function of exosomes is still a matter of debate, but increasing results in various experimental systems suggest their involvement in multiple biological processes. Because both cell‐culture supernatants and biological fluids contain different types of lipid membranes, it is critical to perform high‐quality exosome purification. This unit describes different approaches for exosome purification from various sources, and discusses methods to evaluate the purity and homogeneity of the purified exosome preparations.
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“For electron microscopy (EM) analysis of EVs, samples were prepared as per the protocol described previously72.”
From GAPDH controls extracellular vesicle biogenesis and enhances the therapeutic potential of EV mediated siRNA delivery to the brain (2021) · cites this paper as [72]
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Cites
B lymphocytes secrete antigen-presenting vesicles.
Raposo, G., Nijman, H. W., Stoorvogel, W., Liejendekker, R., Harding, C. V., Melief, C. J., & Geuze, H. J. (1996) · The Journal of experimental medicine · doi:10.1084/jem.183.3.1161 · Cited by 3,546
Self-citationZitvogel, L., Régnault, A., Lozier, A., Wolfers, J., Flament, C., Tenza, D., Ricciardi‐Castagnoli, P., Raposo, G., & Amigorena, S. (1998) · Nature Medicine · doi:10.1038/nm0598-594 · Cited by 2,266
Self-citationIdentification and proteomic profiling of exosomes in human urine
Pisitkun, T., Shen, R., & Knepper, M. A. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0403453101 · Cited by 2,162
Tumor-derived exosomes are a source of shared tumor rejection antigens for CTL cross-priming
Wolfers, J., Lozier, A., Raposo, G., Régnault, A., Théry, C., Masurier, C., Flament, C., Pouzieux, S., Faure, F., Tursz, T., et al. (2001) · Nature Medicine · doi:10.1038/85438 · Cited by 1,643
Self-citationThéry, C., Boussac, M., Véron, P., Ricciardi‐Castagnoli, P., Raposo, G., Garin, J., & Amigorena, S. (2001) · The Journal of Immunology · doi:10.4049/jimmunol.166.12.7309 · Cited by 1,564
Self-citationHeijnen, H. F. G., Schiel, A. E., Fijnheer, R., Geuze, H. J., & Sixma, J. J. (1999) · Blood · doi:10.1182/blood.v94.11.3791 · Cited by 1,488
Exosomal-like vesicles are present in human blood plasma
Caby, M.-P., Lankar, D., Vincendeau-Scherrer, C., Raposo, G., & Bonnerot, C. (2005) · International Immunology · doi:10.1093/intimm/dxh267 · Cited by 1,348
Self-citationPan, B., Teng, K., Wu, C., Adam, M., & Johnstone, R. M. (1985) · The Journal of Cell Biology · doi:10.1083/jcb.101.3.942 · Cited by 1,242
Escola, J.-M., Kleijmeer, M. J., Stoorvogel, W., Griffith, J. M., Yoshie, O., & Geuze, H. J. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.32.20121 · Cited by 1,228
Molecular Characterization of Dendritic Cell-Derived Exosomes
Théry, C., Régnault, A., Garin, J., Wolfers, J., Zitvogel, L., Ricciardi‐Castagnoli, P., Raposo, G., & Amigorena, S. (1999) · The Journal of Cell Biology · doi:10.1083/jcb.147.3.599 · Cited by 1,065
Self-citation
Research works printed but not linked: 1
- Raposo G., 1997, Handbook of Experimental Immunology, 1
Cited by
Sorted by citations
Théry, C., Witwer, K. W., Aikawa, E., Alcaraz, M. J., Anderson, J. D., Andriantsitohaina, R., Antoniou, A., Arab, T., Archer, F., Atkin‐Smith, G. K., et al. (2018) · Journal of Extracellular Vesicles · doi:10.1080/20013078.2018.1535750 · Cited by 11,099
Self-citationShedding light on the cell biology of extracellular vesicles
van Niel, G., D'Angelo, G., & Raposo, G. (2018) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2017.125 · Cited by 8,369
Self-citationExtracellular vesicles: Exosomes, microvesicles, and friends
Raposo, G., & Stoorvogel, W. (2013) · Journal of Cell Biology · doi:10.1083/jcb.201211138 · Cited by 8,099
Self-citationBiogenesis, Secretion, and Intercellular Interactions of Exosomes and Other Extracellular Vesicles
Colombo, M., Raposo, G., & Théry, C. (2014) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev-cellbio-101512-122326 · Cited by 6,186
Self-citationDelivery of siRNA to the mouse brain by systemic injection of targeted exosomes
Alvarez-Erviti, L., Seow, Y., Yin, H., Betts, C., Lakhal, S., & Wood, M. J. A. (2011) · Nature Biotechnology · doi:10.1038/nbt.1807 · Cited by 4,930
Welsh, J. A., Goberdhan, D. C. I., O'Driscoll, L., Buzas, E. I., Blenkiron, C., Bussolati, B., Cai, H., Di Vizio, D., Driedonks, T. A. P., Erdbrügger, U., et al. (2024) · Journal of Extracellular Vesicles · doi:10.1002/jev2.12404 · Cited by 4,663
Self-citationMembrane vesicles as conveyors of immune responses
Théry, C., Ostrowski, M., & Segura, É. (2009) · Nature reviews. Immunology · doi:10.1038/nri2567 · Cited by 3,996
Self-citationCommunication by Extracellular Vesicles: Where We Are and Where We Need to Go
Tkach, M., & Théry, C. (2016) · Cell · doi:10.1016/j.cell.2016.01.043 · Cited by 3,444
Self-citationExtracellular vesicles: biology and emerging therapeutic opportunities
EL Andaloussi, S., Mäger, I., Breakefield, X. O., & Wood, M. J. A. (2013) · Nature Reviews Drug Discovery · doi:10.1038/nrd3978 · Cited by 3,571
Kowal, J., Arras, G., Colombo, M., Jouve, M., Morath, J. P., Primdal-Bengtson, B., Dingli, F., Loew, D., Tkach, M., & Théry, C. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1521230113 · Cited by 3,426
Self-citationExosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Chen, G., Huang, A. C., Zhang, W., Zhang, G., Wu, M., Xu, W., Yu, Z., Yang, J., Wang, B., Sun, H., et al. (2018) · Nature · doi:10.1038/s41586-018-0392-8 · Cited by 2,813
Glypican-1 identifies cancer exosomes and detects early pancreatic cancer
Melo, S. A., Luecke, L. B., Kahlert, C., Fernandez, A. F., Gammon, S. T., Kaye, J., LeBleu, V. S., Mittendorf, E. A., Weitz, J., Rahbari, N., et al. (2015) · Nature · doi:10.1038/nature14581 · Cited by 2,880
Rab27a and Rab27b control different steps of the exosome secretion pathway
Ostrowski, M., Carmo, N., Krumeich, S., Fanget, I., Raposo, G., Savina, A., Moita, C. F., Schauer, K., Hume, A. N., Freitas, R. P., et al. (2009) · Nature Cell Biology · doi:10.1038/ncb2000 · Cited by 2,761
Self-citationMicroRNAs are transported in plasma and delivered to recipient cells by high-density lipoproteins
Vickers, K. C., Palmisano, B. T., Shoucri, B. M., Shamburek, R. D., & Remaley, A. T. (2011) · Nature Cell Biology · doi:10.1038/ncb2210 · Cited by 2,741
Lötvall, J., Hill, A. F., Hochberg, F., Buzás, E. I., Di Vizio, D., Gardiner, C., Gho, Y. S., Kurochkin, I. V., Mathivanan, S., Quesenberry, P., et al. (2014) · Journal of Extracellular Vesicles · doi:10.3402/jev.v3.26913 · Cited by 2,451
Self-citationExosomes facilitate therapeutic targeting of oncogenic KRAS in pancreatic cancer
Kamerkar, S., LeBleu, V. S., Sugimoto, H., Yang, S., Ruivo, C. F., Melo, S. A., Lee, J. J., & Kalluri, R. (2017) · Nature · doi:10.1038/nature22341 · Cited by 2,639
Witwer, K. W., Buzás, E. I., Bemis, L., Bora, A., Lässer, C., Lötvall, J. O., Hoen, E. N. M. N., Piper, M. G., Sivaraman, S., Skog, J., et al. (2013) · Journal of Extracellular Vesicles · doi:10.3402/jev.v2i0.20360 · Cited by 2,513
Self-citationCurrent knowledge on exosome biogenesis and release
Hessvik, N. P., & Llorente, A. (2018) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-017-2595-9 · Cited by 2,362
Exosomes – vesicular carriers for intercellular communication
Simons, M., & Raposo, G. (2009) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2009.03.007 · Cited by 2,410
Self-citationExosome secreted by MSC reduces myocardial ischemia/reperfusion injury
Lai, R. C., Arslan, F., Lee, M. M., Sze, N. S. K., Choo, A., Chen, T. S., Salto-Tellez, M., Timmers, L., Lee, C. N., El Oakley, R. M., et al. (2010) · Stem Cell Research · doi:10.1016/j.scr.2009.12.003 · Cited by 2,215
Exosomes: biogenesis, biologic function and clinical potential
Zhang, Y., Liu, Y., Liu, H., & Tang, W. H. (2019) · Cell & Bioscience · doi:10.1186/s13578-019-0282-2 · Cited by 2,119
Membrane vesicles, current state-of-the-art: emerging role of extracellular vesicles
György, B., Szabó, T. G., Pásztói, M., Pál, Z., Misják, P., Aradi, B., László, V., Pállinger, É., Pap, E., Kittel, Á., et al. (2011) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-011-0689-3 · Cited by 2,160
Exosomes as drug delivery vehicles for Parkinson’s disease therapy
Haney, M. J., Klyachko, N. L., Zhao, Y., Gupta, R., Plotnikova, E. G., He, Z., Patel, T., Piroyan, A., Sokolsky, M., Kabanov, A. V., et al. (2015) · Journal of Controlled Release · doi:10.1016/j.jconrel.2015.03.033 · Cited by 2,009
Progress in Exosome Isolation Techniques
Li, P., Kaslan, M., Lee, S. H., Yao, J. D., & Gao, Z. (2017) · Theranostics · doi:10.7150/thno.18133 · Cited by 2,019
Vlassov, A. V., Magdaleno, S. M., Setterquist, R. A., & Conrad, R. (2012) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2012.03.017 · Cited by 1,979
Tian, Y., Li, S., Song, J., Ji, T., Zhu, M., Anderson, G. J., Wei, J., & Nie, G. (2013) · Biomaterials · doi:10.1016/j.biomaterials.2013.11.083 · Cited by 1,841
Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells
Mittelbrunn, M., Gutiérrez‐Vázquez, C., Villarroya‐Beltri, C., González, S., Sánchez‐Cabo, F., González, M. A., Bernad, A., & Sánchez‐Madrid, F. (2011) · Nature Communications · doi:10.1038/ncomms1285 · Cited by 1,818
Optimized exosome isolation protocol for cell culture supernatant and human plasma
Lobb, R. J., Becker, M., Wen, S., Wong, C. S. F., Wiegmans, A. P., Leimgruber, A., & Möller, A. (2015) · Journal of Extracellular Vesicles · doi:10.3402/jev.v4.27031 · Cited by 1,763
New Technologies for Analysis of Extracellular Vesicles
Shao, H., Im, H., Castro, C. M., Breakefield, X. O., Weissleder, R., & Lee, H. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00534 · Cited by 1,697
Depletion of microglia and inhibition of exosome synthesis halt tau propagation
Asai, H., Ikezu, S., Tsunoda, S., Medalla, M., Luebke, J., Haydar, T., Wolozin, B., Butovsky, O., Kügler, S., & Ikezu, T. (2015) · Nature Neuroscience · doi:10.1038/nn.4132 · Cited by 1,591
Zhang, H., Freitas, D. P., Kim, H. S., Fabijanic, K. I., Li, Z., Chen, H., Mark, M. T., Molina, H., Benito‐Martín, A., Bojmar, L., et al. (2018) · Nature Cell Biology · doi:10.1038/s41556-018-0040-4 · Cited by 1,666
Applying extracellular vesicles based therapeutics in clinical trials – an ISEV position paper
Lener, T., Gimona, M., Aigner, L., Börger, V., Buzas, E., Camussi, G., Chaput, N., Chatterjee, D., Court, F. A., del Portillo, H. A., et al. (2015) · Journal of Extracellular Vesicles · doi:10.3402/jev.v4.30087 · Cited by 1,491
Self-citationAdipose-derived circulating miRNAs regulate gene expression in other tissues
Thomou, T., Mori, M. A., Dreyfuss, J. M., Konishi, M., Sakaguchi, M., Wolfrum, C., Rao, T. N., Winnay, J. N., Garcia-Martin, R., Grinspoon, S. K., et al. (2017) · Nature · doi:10.1038/nature21365 · Cited by 1,539
Cancer Exosomes Perform Cell-Independent MicroRNA Biogenesis and Promote Tumorigenesis
Melo, S. A., Sugimoto, H., O’Connell, J. T., Kato, N., Villanueva, A., Vidal, A., Qiu, L., Vitkin, E. I., Perelman, L. T., Melo, C. A., et al. (2014) · Cancer Cell · doi:10.1016/j.ccell.2014.09.005 · Cited by 1,566
Development of exosome-encapsulated paclitaxel to overcome MDR in cancer cells
Kim, M. S., Haney, M. J., Zhao, Y., Mahajan, V., Deygen, I., Klyachko, N. L., Inskoe, E., Piroyan, A., Sokolsky, M., Okolie, O., et al. (2016) · Nanomedicine: Nanotechnology, Biology and Medicine · doi:10.1016/j.nano.2015.10.012 · Cited by 1,410
Cancer-secreted miR-105 destroys vascular endothelial barriers to promote metastasis
Zhou, W., Fong, M. Y., Min, Y., Somlo, G., Liu, L., Palomares, M. R., Yu, Y., Chow, A., O’Connor, S. T. F., Chin, A. R., et al. (2014) · Cancer Cell · doi:10.1016/j.ccr.2014.03.007 · Cited by 1,476
Colombo, M., Moita, C. F., van Niel, G., Kowal, J., Vigneron, J., Benaroch, P., Manel, N., Moita, L. F., Théry, C., & Raposo, G. (2013) · Journal of Cell Science · doi:10.1242/jcs.128868 · Cited by 1,534
Self-citationAkers, J. C., Gonda, D., Kim, R., Carter, B. S., & Chen, C. C. (2013) · Journal of Neuro-Oncology · doi:10.1007/s11060-013-1084-8 · Cited by 1,423
“Purification by density gradient using sucrose gradients has also been reported [17].”
A proteomic atlas of senescence-associated secretomes for aging biomarker development
Basisty, N., Kale, A., Jeon, O. H., Kuehnemann, C., Payne, T., Rao, C., Holtz, A., Shah, S., Sharma, V., Ferrucci, L., et al. (2020) · PLOS Biology · doi:10.1371/journal.pbio.3000599 · Cited by 1,348
Sizing and phenotyping of cellular vesicles using Nanoparticle Tracking Analysis
Dragovic, R., Gardiner, C., Brooks, A. S., Tannetta, D., Ferguson, D. J. P., Hole, P., Carr, B., Redman, C. W. G., Harris, A. L., Dobson, P. J., et al. (2011) · Nanomedicine Nanotechnology Biology and Medicine · doi:10.1016/j.nano.2011.04.003 · Cited by 1,368
Label-free detection and molecular profiling of exosomes with a nano-plasmonic sensor
Im, H., Shao, H., Park, Y. I., Peterson, V. M., Castro, C. M., Weissleder, R., & Lee, H. (2014) · Nature Biotechnology · doi:10.1038/nbt.2886 · Cited by 1,331
Zhang, Y., Bi, J., Huang, J., Tang, Y., Du, S., & Li, P. (2020) · International Journal of Nanomedicine · doi:10.2147/ijn.s264498 · Cited by 1,323
Extracellular vesicles for drug delivery.
Vader, P., Mol, E. A., Pasterkamp, G., & Schiffelers, R. M. (2016) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2016.02.006 · Cited by 1,319
Extracellular Vesicles: Composition, Biological Relevance, and Methods of Study
Zaborowski, M. P., Balaj, L., Breakefield, X. O., & Lai, C. P. (2015) · BioScience · doi:10.1093/biosci/biv084 · Cited by 1,336
Exosome Theranostics: Biology and Translational Medicine
He, C., Zheng, S., Luo, Y., & Wang, B. (2017) · Theranostics · doi:10.7150/thno.21945 · Cited by 1,271
Isolation of Extracellular Vesicles: General Methodologies and Latest Trends
Konoshenko, M. Y., Lekchnov, E. A., Vlassov, A. V., & Laktionov, P. P. (2018) · BioMed Research International · doi:10.1155/2018/8545347 · Cited by 1,302
Extracellular Vesicle and Particle Biomarkers Define Multiple Human Cancers
Hoshino, A., Kim, H. S., Bojmar, L., Gyan, K. E., Cioffi, M., Hernandez, J. M., Zambirinis, C. P., Rodrigues, G., Molina, H., Heissel, S., et al. (2020) · Cell · doi:10.1016/j.cell.2020.07.009 · Cited by 1,275
Single‐step isolation of extracellular vesicles by size‐exclusion chromatography
Böing, A. N., van der Pol, E., Grootemaat, A. E., Coumans, F. A. W., Sturk, A., & Nieuwland, R. (2014) · Journal of Extracellular Vesicles · doi:10.3402/jev.v3.23430 · Cited by 1,228
Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration
Luga, V., Zhang, L., Viloria-Petit, A. M., Ogunjimi, A. A., Inanlou, M. R., Chiu, E., Buchanan, M., Hosein, A. N., Basik, M., & Wrana, J. L. (2012) · Cell · doi:10.1016/j.cell.2012.11.024 · Cited by 1,311
Secreted Monocytic miR-150 Enhances Targeted Endothelial Cell Migration
Zhang, Y., Liu, D., Chen, X., Li, J., Li, L., Bian, Z., Sun, F., Lu, J., Yin, Y., Cai, X. F., et al. (2010) · Molecular Cell · doi:10.1016/j.molcel.2010.06.010 · Cited by 1,219
Tauro, B. J., Greening, D. W., Mathias, R. A., Ji, H. L., Mathivanan, S., Scott, A. M., & Simpson, R. J. (2012) · Methods · doi:10.1016/j.ymeth.2012.01.002 · Cited by 1,214
Microenvironment-induced PTEN loss by exosomal microRNA primes brain metastasis outgrowth
Zhang, L., Zhang, S., Yao, J., Lowery, F. J., Zhang, Q., Huang, W.-C., Li, P., Li, M., Wang, X., Zhang, C., et al. (2015) · Nature · doi:10.1038/nature15376 · Cited by 1,175
Surface functionalized exosomes as targeted drug delivery vehicles for cerebral ischemia therapy
Tian, T., Zhang, H.-X., He, C.-P., Fan, S., Zhu, Y.-L., Qi, C., Huang, N.-P., Xiao, Z.-D., Lu, Z.-H., Tannous, B. A., et al. (2018) · Biomaterials · doi:10.1016/j.biomaterials.2017.10.012 · Cited by 1,154
Emmanouilidou, E., Melachroinou, K., Roumeliotis, T., Garbis, S. D., Ntzouni, M., Margaritis, L. H., Stefanis, L., & Vekrellis, K. (2010) · The Journal of Neuroscience · doi:10.1523/jneurosci.5699-09.2010 · Cited by 1,115
“To confirm that the CM of -synuclein-expressing cells contained exosomes, we isolated pure exosomes from the CM using a validated differential ultracentrifugation procedure (Théry et al., 2006).”
BackgroundMethodResultInfluentialGurunathan, S., Kang, M.-H., Jeyaraj, M., Qasim, M., & Kim, J. (2019) · Cells · doi:10.3390/cells8040307 · Cited by 1,160
Using exosomes, naturally-equipped nanocarriers, for drug delivery
Batrakova, E. V., & Kim, M. S. (2015) · Journal of Controlled Release · doi:10.1016/j.jconrel.2015.07.030 · Cited by 1,041
Fong, M. Y., Zhou, W., Liu, L., Alontaga, A. Y., Chandra, M., Ashby, J., Chow, A., O’Connor, S. T. F., Li, S., Chin, A. R., et al. (2015) · Nature Cell Biology · doi:10.1038/ncb3094 · Cited by 1,118
Exosome Secretion: Molecular Mechanisms and Roles in Immune Responses
Bobrie, A., Colombo, M., Raposo, G., & Théry, C. (2011) · Traffic · doi:10.1111/j.1600-0854.2011.01225.x · Cited by 1,148
Self-citationQuantitative and stoichiometric analysis of the microRNA content of exosomes
Chevillet, J. R., Kang, Q., Ruf, I. K., Briggs, H., Vojtech, L. N., Hughes, S. M., Cheng, H. H., Arroyo, J. D., Meredith, E. K., Gallichotte, E. N., et al. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1408301111 · Cited by 1,137
Hypoxic enhancement of exosome release by breast cancer cells
King, H. W., Michael, M. Z., & Gleadle, J. M. (2012) · BMC Cancer · doi:10.1186/1471-2407-12-421 · Cited by 1,045
Advances in therapeutic applications of extracellular vesicles
Wiklander, O. P. B., Brennan, M. Á., Lötvall, J., Breakefield, X. O., & EL Andaloussi, S. (2019) · Science Translational Medicine · doi:10.1126/scitranslmed.aav8521 · Cited by 1,039
Exosomes Released by Melanoma Cells Prepare Sentinel Lymph Nodes for Tumor Metastasis
Hood, J. L., San, R. S., & Wickline, S. A. (2011) · Cancer Research · doi:10.1158/0008-5472.can-10-4455 · Cited by 1,056
Yang, D., Zhang, W., Zhang, H., Zhang, F., Chen, L., Ma, L., Larcher, L. M., Chen, S., Liu, N., Zhao, Q., et al. (2020) · Theranostics · doi:10.7150/thno.41580 · Cited by 1,079
Lipids in exosomes: current knowledge and the way forward
Skotland, T., Sandvig, K., & Llorente, A. (2017) · Progress in Lipid Research · doi:10.1016/j.plipres.2017.03.001 · Cited by 1,030
Kahlert, C., Melo, S. A., Protopopov, A. I., Tang, J., Seth, S., Koch, M., Zhang, J., Weitz, J., Chin, L., Futreal, P. A., et al. (2014) · Journal of Biological Chemistry · doi:10.1074/jbc.c113.532267 · Cited by 1,058
Methodological Guidelines to Study Extracellular Vesicles
Coumans, F. A. W., Brisson, A. R., Buzás, E. I., Dignat‐George, F., Drees, E. E. E., Andaloussi, S. E., Emanueli, C., Gąsecka, A., Hendrix, A., Hill, A. F., et al. (2017) · Circulation Research · doi:10.1161/circresaha.117.309417 · Cited by 1,052
Active Wnt proteins are secreted on exosomes
Gross, J. C., Chaudhary, V., Bartscherer, K., & Boutros, M. (2012) · Nature Cell Biology · doi:10.1038/ncb2574 · Cited by 1,025
Mesenchymal stem cells use extracellular vesicles to outsource mitophagy and shuttle microRNAs
Phinney, D. G., Di Giuseppe, M., Njah, J., Sala, E., Shiva, S., St Croix, C. M., Stolz, D. B., Watkins, S. C., Di, Y. P., Leikauf, G. D., et al. (2015) · Nature Communications · doi:10.1038/ncomms9472 · Cited by 1,007
Fang, T., Lv, H., Lv, G., Li, T., Wang, C., Han, Q., Yu, L., Su, B., Guo, L., Huang, S., et al. (2018) · Nature Communications · doi:10.1038/s41467-017-02583-0 · Cited by 995
Willms, E., Cabañas, C., Mäger, I., Wood, M. J. A., & Vader, P. (2018) · Frontiers in Immunology · doi:10.3389/fimmu.2018.00738 · Cited by 1,030
Saman, S., Kim, W., Raya, M., Visnick, Y., Miro, S., Saman, S., Jackson, B., McKee, A. C., Alvarez, V. E., Lee, N. C. Y., et al. (2012) · Journal of Biological Chemistry · doi:10.1074/jbc.m111.277061 · Cited by 985
Bang, C., Bátkai, S., Dangwal, S., Gupta, S. K., Foinquinos, A., Holzmann, A., Just, A., Remke, J., Zimmer, K., Zeug, A., et al. (2014) · Journal of Clinical Investigation · doi:10.1172/jci70577 · Cited by 995
Mathieu, M., Névo, N., Jouve, M., Valenzuela, J. I., Maurin, M., Verweij, F. J., Palmulli, R., Lankar, D., Dingli, F., Loew, D., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-24384-2 · Cited by 910
Self-citationThe impact of disparate isolation methods for extracellular vesicles on downstream RNA profiling
Deun, J. V., Mestdagh, P., Sormunen, R. T., Cocquyt, V., Vermaelen, K., Vandesompele, J., Bracke, M., Wever, O. D., & Hendrix, A. (2014) · Journal of Extracellular Vesicles · doi:10.3402/jev.v3.24858 · Cited by 987
Exosomes and microvesicles: extracellular vesicles for genetic information transfer and gene therapy
Lee, Y., Andaloussi, S. E., & Wood, M. J. A. (2012) · Human Molecular Genetics · doi:10.1093/hmg/dds317 · Cited by 958
Bovine milk-derived exosomes for drug delivery
Munagala, R., Aqil, F., Jeyabalan, J., & Gupta, R. C. (2015) · Cancer Letters · doi:10.1016/j.canlet.2015.10.020 · Cited by 936
van der Pol, E., Coumans, F. A. W., Grootemaat, A. E., Gardiner, C., Sargent, I. L., Harrison, P., Sturk, A., van Leeuwen, T. G. J. M., & Nieuwland, R. (2014) · Journal of Thrombosis and Haemostasis · doi:10.1111/jth.12602 · Cited by 947
Mesenchymal stem cell-derived extracellular vesicles: novel frontiers in regenerative medicine
Keshtkar, S., Azarpira, N., & Ghahremani, M. H. (2018) · Stem Cell Research & Therapy · doi:10.1186/s13287-018-0791-7 · Cited by 938
Brennan, K., Martin, K., Fitzgerald, S. P., O’Sullivan, J., Wu, Y., Blanco, A., Richardson, C., & Gee, M. M. M. (2020) · Scientific Reports · doi:10.1038/s41598-020-57497-7 · Cited by 943
Extracellular vesicle isolation and characterization: toward clinical application
Xu, R., Greening, D. W., Zhu, H., Takahashi, N., & Simpson, R. J. (2016) · Journal of Clinical Investigation · doi:10.1172/jci81129 · Cited by 893
Focus on Extracellular Vesicles: Introducing the Next Small Big Thing
Kalra, H., Drummen, G. P. C., & Mathivanan, S. (2016) · International Journal of Molecular Sciences · doi:10.3390/ijms17020170 · Cited by 906
As we wait: coping with an imperfect nomenclature for extracellular vesicles
Gould, S. J., & Raposo, G. (2013) · Journal of Extracellular Vesicles · doi:10.3402/jev.v2i0.20389 · Cited by 864
Self-citationSokolova, V., Ludwig, A., Hornung, S., Rotan, O., Horn, P. A., Epple, M., & Giebel, B. (2011) · Colloids and Surfaces B Biointerfaces · doi:10.1016/j.colsurfb.2011.05.013 · Cited by 869
Exosomal cell-to-cell transmission of alpha synuclein oligomers
Danzer, K. M., Kranich, L. R., Ruf, W. P., Cagsal‐Getkin, O., Winslow, A. R., Zhu, L., Vanderburg, C. R., & McLean, P. J. (2012) · Molecular Neurodegeneration · doi:10.1186/1750-1326-7-42 · Cited by 880
Cancer Exosomes Trigger Fibroblast to Myofibroblast Differentiation
Webber, J. P., Steadman, R., Mason, M. D., Tabi, Z., & Clayton, A. (2010) · Cancer Research · doi:10.1158/0008-5472.can-10-1722 · Cited by 899
Self-citationRecord, M., Carayon, K., Poirot, M., & Silvente‐Poirot, S. (2013) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2013.10.004 · Cited by 893
Webber, J., & Clayton, A. (2013) · Journal of Extracellular Vesicles · doi:10.3402/jev.v2i0.19861 · Cited by 817
Self-citationExosome Transfer from Stromal to Breast Cancer Cells Regulates Therapy Resistance Pathways
Boelens, M. C., Wu, T. J., Nabet, B. Y., Xu, B., Qiu, Y., Yoon, T., Azzam, D. J., Victor, C. T.-S., Wiemann, B. Z., Ishwaran, H., et al. (2014) · Cell · doi:10.1016/j.cell.2014.09.051 · Cited by 819
Exosomes Derived from Mesenchymal Stem Cells
Yu, B., Zhang, X., & Li, X. (2014) · International Journal of Molecular Sciences · doi:10.3390/ijms15034142 · Cited by 788
Exosomes maintain cellular homeostasis by excreting harmful DNA from cells
Takahashi, A., Okada, R., Nagao, K., Kawamata, Y., Hanyu, A., Yoshimoto, S., Takasugi, M., Watanabe, S., Kanemaki, M. T., Obuse, C., et al. (2017) · Nature Communications · doi:10.1038/ncomms15287 · Cited by 761
Doeppner, T. R., Herz, J., Görgens, A., Schlechter, J., Ludwig, A., Radtke, S., de Miroschedji, K., Horn, P. A., Giebel, B., & Hermann, D. M. (2015) · Stem Cells Translational Medicine · doi:10.5966/sctm.2015-0078 · Cited by 789
Buck, A. H., Coakley, G., Simbari, F., McSorley, H. J., Quintana, J. F., Le Bihan, T., Kumar, S., Abreu-Goodger, C., Lear, M., Harcus, Y., et al. (2014) · Nature Communications · doi:10.1038/ncomms6488 · Cited by 770
Wang, X., Luo, G., Zhang, K., Cao, J., Huang, C., Jiang, T., Liu, B., Su, L., & Qiu, Z. (2018) · Cancer Research · doi:10.1158/0008-5472.can-17-3841 · Cited by 771
Baglio, S. R., Rooijers, K., Koppers‐Lalic, D., Verweij, F. J., Pérez-Lanzón, M., Zini, N., Naaijkens, B. A., Perut, F., Niessen, H. W. M., Baldini, N., et al. (2015) · Stem Cell Research & Therapy · doi:10.1186/s13287-015-0116-z · Cited by 776
Exosomes: Vehicles of Intercellular Signaling, Biomarkers, and Vectors of Cell Therapy
Kourembanas, S. (2015) · Annual Review of Physiology · doi:10.1146/annurev-physiol-021014-071641 · Cited by 761
Protein typing of circulating microvesicles allows real-time monitoring of glioblastoma therapy
Shao, H., Chung, J., Balaj, L., Charest, A., Bigner, D. D., Carter, B. S., Hochberg, F. H., Breakefield, X. O., Weissleder, R., & Lee, H. (2012) · Nature Medicine · doi:10.1038/nm.2994 · Cited by 782
Exosome-delivered microRNAs modulate the inflammatory response to endotoxin
Alexander, M., Hu, R., Runtsch, M. C., Kagele, D. A., Mosbruger, T. L., Tolmachova, T., Seabra, M. C., Round, J. L., Ward, D. M., & O’Connell, R. M. (2015) · Nature Communications · doi:10.1038/ncomms8321 · Cited by 769
Tumor-Derived Exosomes and Their Role in Cancer Progression
Whiteside, T. L. (2016) · Advances in clinical chemistry · doi:10.1016/bs.acc.2015.12.005 · Cited by 775
Exosomal lipid composition and the role of ether lipids and phosphoinositides in exosome biology
Skotland, T., Hessvik, N. P., Sandvig, K., & Llorente, A. (2019) · Journal of Lipid Research · doi:10.1194/jlr.r084343 · Cited by 730
Plant Exosome-like Nanovesicles: Emerging Therapeutics and Drug Delivery Nanoplatforms
Dad, H. A., Gu, T.-W., Zhu, A.-Q., Huang, L., & Peng, L. (2020) · Molecular Therapy · doi:10.1016/j.ymthe.2020.11.030 · Cited by 772
Metadata
- Authors
- Théry, C., Amigorena, S., Raposo, G., & Clayton, A.
- Affiliations
- Institut Curie (FR)
- Institut Curie Paris France
- Cardiff University (GB)
- Venue
- Current Protocols in Cell Biology
- Publisher
- Wiley
- Published
- 2006-03
- Volume
- 30
- Issue
- 1
- Pages
- Unit 3.22-Unit 3.22
- ISSN
- 1934-2500 (ISSN-L)
- 1934-2616
- OpenAlex
- W1877686344
- Cited by
- 5,766
- References
- 30
- Citations by year
- 2026: 206 · 2025: 356 · 2024: 485 · 2023: 502 · 2022: 558 · 2021: 641 · 2020: 620 · 2019: 521 · 2018: 452 · 2017: 390 · 2016: 301 · 2015: 259 · 2014: 177 · 2013: 106 · 2012: 85
- FWCI
- 3.54
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Current Protocols in Cell Biology (publishedVersion)
- PubMed (publishedVersion)
- ORCA Online Research @Cardiff (Cardiff University) (acceptedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Keywords
- exosomes, exosome isolation, multivesicular bodies, cell culture supernatant, biological fluids, exosome characterization, membrane vesicles, exosome function
- MeSH terms
- Animals, Culture Media, Electrophoresis, Polyacrylamide Gel, Flow Cytometry, Humans, Immunohistochemistry, Ultracentrifugation, Cell Culture Techniques, Secretory Vesicles, Microscopy, Electron, Transmission
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