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Aging Research Using Mouse Models
Open accessAbstract
Despite the dramatic increase in human lifespan over the past century, there remains pronounced variability in “health‐span,” or the period of time in which one is generally healthy and free of disease. Much of the variability in health‐span and lifespan is thought to be genetic in origin. Understanding the genetic mechanisms of aging and identifying ways to boost longevity is a primary goal in aging research. Here, we describe a pipeline of phenotypic assays for assessing mouse models of aging. This pipeline includes behavior/cognition testing, body composition analysis, and tests of kidney function, hematopoiesis, and immune function, as well as physical parameters. We also describe study design methods for assessing lifespan and health‐span, and other important considerations when conducting aging research in the laboratory mouse. The tools and assays provided can assist researchers with understanding the correlative relationships between age‐associated phenotypes and, ultimately, the role of specific genes in the aging process. © 2015 by John Wiley & Sons, Inc.
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“As mice age, they undergo characteristic physical and physiological changes, including alopecia, hair greying, kyphosis, decreased body weight, motion in dark phase, and a reduced respiratory exchange ratio (RER) (Ackert-Bicknell et al., 2015; Harkema et al., 2016; Houtkooper et al., 2011; Koks et al., 2016).”
From Loss of epigenetic information as a cause of mammalian aging (2023) · cites this paper as Ackert-Bicknell et al., 2015
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“WT C57BL6/J mice have a median lifespan of ∼129 weeks (Yuan et al., 2012), equivalent to ∼80 years in humans (Ackert-Bicknell et al., 2015)”
From Gene Therapy-Mediated Partial Reprogramming Extends Lifespan and Reverses Age-Related Changes in Aged Mice (2024) · cites this paper as Ackert-Bicknell et al., 2015
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Metadata
- Authors
- Ackert‐Bicknell, C. L., Anderson, L. C., Sheehan, S., Hill, W. G., Chang, B., Churchill, G. A., Chesler, E. J., Korstanje, R., & Peters, L. L.
- Affiliations
- University of Rochester Medicine (US)
- Jackson Laboratory (US)
- Beth Israel Deaconess Medical Center (US)
- ORCID
- Venue
- Current Protocols in Mouse Biology
- Publisher
- Wiley
- Published
- 2015-06
- Volume
- 5
- Issue
- 2
- Pages
- 95-133
- ISSN
- 2161-2617 (ISSN-L)
- PMC
- PMC4590775
- OpenAlex
- W1519109490
- Cited by
- 135
- References
- 41
- Citations by year
- 2026: 13 · 2025: 13 · 2024: 19 · 2023: 20 · 2022: 18 · 2021: 23 · 2020: 8 · 2019: 8 · 2018: 6 · 2017: 4 · 2016: 3
- FWCI
- 1.74
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Current Protocols in Mouse Biology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- The Mouseion at the JAXlibrary (Jackson Laboratory) (submittedVersion)
- Funders
- National Institutes of Health (5p20dk097818-02, 5r01ey019943-13)
- National Institute on Aging (AG-038070, P30AG038070)
- National Eye Institute (EY019943, R01-EY019943)
- National Cancer Institute (P30CA034196)
- National Institute of Diabetes and Digestive and Kidney Diseases (DK097818, DK083299, P20-DK-097818, R01DK083299)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Telomeres, Telomerase, and Senescence
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Antioxidants, Aging, Portulaca oleracea
- Keywords
- aging, mouse models of aging, healthspan, lifespan, longevity, phenotypic assays, body composition, renal function, hematopoiesis, immune function, research design
- MeSH terms
- Aging, Animals, Animal Structures, Behavior, Animal, Disease Models, Animal, Humans, Laboratory Animal Science, Longevity, Research Design, Mice
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