The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.
PEI–PEG–Chitosan‐Copolymer‐Coated Iron Oxide Nanoparticles for Safe Gene Delivery: Synthesis, Complexation, and Transfection
Open accessAbstract
Gene therapy offers the potential of mediating disease through modification of specific cellular functions of target cells. However, effective transport of nucleic acids to target cells with minimal side effects remains a challenge despite the use of unique viral and non‐viral delivery approaches. Here, a non‐viral nanoparticle gene carrier that demonstrates effective gene delivery and transfection both in vitro and in vivo is presented. The nanoparticle system (NP–CP–PEI) is made of a superparamagnetic iron oxide nanoparticle (NP), which enables magnetic resonance imaging, coated with a novel copolymer (CP–PEI) comprised of short chain polyethylenimine (PEI) and poly(ethylene glycol) (PEG) grafted to the natural polysaccharide, chitosan (CP), which allows efficient loading and protection of the nucleic acids. The function of each component material in this nanoparticle system is illustrated by comparative studies of three nanoparticle systems of different surface chemistries, through material property characterization, DNA loading and transfection analyses, and toxicity assessment. Significantly, NP–CP–PEI demonstrates an innocuous toxic profile and a high level of expression of the delivered plasmid DNA in a C6 xenograft mouse model, making it a potential candidate for safe in vivo delivery of DNA for gene therapy.
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Cites
1 retracted
RETRACTED: Polyethylenimine nanoparticles as efficient transfecting agents for mammalian cells
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RetractedMagnetic nanoparticles in MR imaging and drug delivery
Sun, C., Lee, J. S. H., & Zhang, M. (2008) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2008.03.018 · Cited by 2,594
Self-citationDesign and development of polymers for gene delivery
Pack, D. W., Hoffman, A. S., Pun, S., & Stayton, P. S. (2005) · Nature Reviews Drug Discovery · doi:10.1038/nrd1775 · Cited by 2,568
Chitosan-DNA nanoparticles as gene carriers: synthesis, characterization and transfection efficiency
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Poly(ethylenimine) and its role in gene delivery
Godbey, W. T., Wu, K. K., & Mikos, A. G. (1999) · Journal of Controlled Release · doi:10.1016/s0168-3659(99)00090-5 · Cited by 1,199
A two-stage poly(ethylenimine)-mediated cytotoxicity: implications for gene transfer/therapy
Moghimi, S. M., Symonds, P., Murray, J. C., Hunter, A. C., Debska, G., & Szewczyk, A. (2005) · Molecular Therapy · doi:10.1016/j.ymthe.2005.02.010 · Cited by 1,092
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Polyethylenimine-based non-viral gene delivery systems
Lungwitz, U., Breunig, M., Blunk, T., & Göpferich, A. (2005) · European Journal of Pharmaceutics and Biopharmaceutics · doi:10.1016/j.ejpb.2004.11.011 · Cited by 983
Magnetofection: enhancing and targeting gene delivery by magnetic force in vitro and in vivo
Scherer, F., Anton, M., Schillinger, U., Henke, J., Bergemann, C., Krüger, A., Gänsbacher, B., & Plank, C. (2002) · Gene Therapy · doi:10.1038/sj.gt.3301624 · Cited by 932
Cited by
Reddy, L. H., Arias, J. L., Nicolas, J., & Couvreur, P. (2012) · Chemical Reviews · doi:10.1021/cr300068p · Cited by 2,010
Synthesis, Functionalization, and Biomedical Applications of Multifunctional Magnetic Nanoparticles
Hao, R., Xing, R., Xu, Z., Hou, Y., Gao, S., & Sun, S. (2010) · Advanced Materials · doi:10.1002/adma.201000260 · Cited by 1,372
In vivo delivery, pharmacokinetics, biodistribution and toxicity of iron oxide nanoparticles
Arami, H., Khandhar, A., Liggitt, D., & Krishnan, K. M. (2015) · Chemical Society Reviews · doi:10.1039/c5cs00541h · Cited by 830
Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts
Stewart, M. P., Langer, R., & Jensen, K. F. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00678 · Cited by 747
Gold Nanoparticles for Nucleic Acid Delivery
Ding, Y., Jiang, Z., Saha, K., Kim, C. S., Kim, S. T., Landis, R. F., & Rotello, V. M. (2014) · Molecular Therapy · doi:10.1038/mt.2014.30 · Cited by 521
Metadata
- Authors
- Kievit, F. M., Veiseh, O., Bhattarai, N., Fang, C., Gunn, J. W., Lee, D., Ellenbogen, R. G., Olson, J. M., & Zhang, M.
- Venue
- Advanced Functional Materials
- Publisher
- Wiley
- Published
- 2009-07-16
- Volume
- 19
- Issue
- 14
- Pages
- 2244-2251
- ISSN
- 1616-301X (ISSN-L)
- 1616-3028
- OpenAlex
- W2055622529
- Cited by
- 381
- References
- 44
- FWCI
- 10.44
- Type
- journal article
- Language
- en
- Open access
- green
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- The paper
- Kievit, F. M., Veiseh, O., Bhattarai, N., Fang, C., Gunn, J. W., Lee, D., Ellenbogen, R. G., Olson, J. M., & Zhang, M. (2009). PEI–PEG–Chitosan‐Copolymer‐Coated Iron Oxide Nanoparticles for Safe Gene Delivery: Synthesis, Complexation, and Transfection. Advanced Functional Materials. https://doi.org/10.1002/adfm.200801844
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