Cited by 3523D Printing in Biomedical ResearchTissue Engineering and Regenerative MedicineNanomaterials and Printing Technologies

Human Skin 3D Bioprinting Using Scaffold‐Free Approach

Pourchet, Léa J., Thepot, Amélie, Albouy, Marion, Courtial, Edwin J., Boher, Aurélie, Blum, Loïc J., Marquette, Christophe A.·2017·Advanced Healthcare Materials

Abstract

Organ in vitro synthesis is one of the last bottlenecks between tissue engineering and transplantation of synthetic organs. Bioprinting has proven its capacity to produce 3D objects composed of living cells but highly organized tissues such as full thickness skin (dermis + epidermis) are rarely attained. The focus of the present study is to demonstrate the capability of a newly developed ink formulation and the use of an open source printer, for the production of a really complete skin model. Proofs are given through immunostaining and electronic microscopy that the bioprinted skin presents all characteristics of human skin, both at the molecular and macromolecular level. Finally, the printability of large skin objects is demonstrated with the printing of an adult‐size ear.

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Crossref · 10.1002/adhm.201601101

Cites

17 of 17 references verified0 citation errors · 0 not checkable

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  • HSE composition and culture conditions vary greatly from lab to lab, which can affect interpretation of similar experiments.

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Authors
Pourchet, L. J., Thepot, A., Albouy, M., Courtial, E. J., Boher, A., Blum, L. J., & Marquette, C. A.
Affiliations
  • Lyon 1 Université (FR)
  • Hôpital Edouard Herriot (FR)
  • 3d.FAB, Univ Lyon Université Lyon1 CNRS, INSA, CPE‐Lyon ICBMS UMR 5246, 43, Bd du 11 novembre 1918 69622 Villeurbanne cedex France
Corresponding author
Marquette, Christophe A.
Venue
Advanced Healthcare Materials
Publisher
Wiley
Published
2016-12-15
Volume
6
Issue
4
Pages
1601101
ISSN
  • 2192-2640 (ISSN-L)
  • 2192-2659
OpenAlex
W2561559726
Cited by
352
References
17
Citations by year
2026: 18 · 2025: 26 · 2024: 39 · 2023: 40 · 2022: 47 · 2021: 51 · 2020: 37 · 2019: 45 · 2018: 32 · 2017: 16
FWCI
14.95
Type
journal article
Language
en
Open access
closed
Locations
  • Advanced Healthcare Materials (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Physical Sciences › Engineering › Electrical and Electronic Engineering › Nanomaterials and Printing Technologies
Keywords
full-thickness skin, open-source 3D printer, skin model, printability, tissue engineering
MeSH terms
Epidermal Cells, Animals, Ear, Epidermis, Humans, Dermis, NIH 3T3 Cells, Mice, Printing, Three-Dimensional

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