In vivo residue-specific dopa-incorporated engineered mussel bioglue with enhanced adhesion and water resistance
Abstract
Misaminoacylation of 3,4-dihydroxyphenylalanine (Dopa) molecules to tRNA(Tyr) by endogenous tyrosyl-tRNA synthetase allowed the quantitative replacement of tyrosine residues with a yield of over 90 % by an in vivo residue-specific incorporation strategy, to create, for the first time, engineered mussel adhesive proteins (MAPs) in Escherichia coli with a very high Dopa content, close to that of natural MAPs. The Dopa-incorporated MAPs exhibited a superior surface adhesion and water resistance ability by assistance of Dopa-mediated interactions including the oxidative Dopa cross-linking, and furthermore, showed underwater adhesive properties comparable to those of natural MAPs. These results propose promising use of Dopa-incorporated engineered MAPs as bioglues or adhesive hydrogels for practical underwater applications.
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An in vivo residue-specific incorporation strategy created mussel adhesive proteins with a high DOPA content, showing promise in the production of mussel-inspired macromolecules.
“Recently, an in vivo residue-specific incorporation strategy was proposed. This strategy created mussel adhesive proteins with a high DOPA content [59], showing promise in the production of mussel-inspired macromolecules.”
From Mussel-Inspired Injectable Adhesive Hydrogels for Biomedical Applications (2024) · cites this paper as [59]
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Recent progress in synthesis and application of mussel-inspired adhesives
Guo, Q., Chen, J., Wang, J., Zeng, H., & Yu, J. (2020) · Nanoscale · doi:10.1039/c9nr09780e · Cited by 370 · see citing works
Self-citationMussel-Inspired Injectable Adhesive Hydrogels for Biomedical Applications
Dou, W., Zeng, X., Zhu, S., Zhu, Y., Liu, H., & Li, S. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms25169100 · Cited by 52 · see citing works
“This strategy created mussel adhesive proteins with a high DOPA content [59], showing promise in the production of mussel-inspired macromolecules.”
BackgroundLiu, X., Yang, X., Yang, Z., Luo, J., Tian, X., Liu, K., Kou, S., & Sun, F. (2018) · ACS Applied Nano Materials · doi:10.1021/acsanm.8b00077 · Cited by 32 · see citing works
Environment-dependent adhesive behaviors of mussel-inspired coordinate-crosslinked bioadhesives
Li, S., Chen, N., Li, Y., Zhao, J., Hou, X., & Yuan, X. (2020) · Macromolecular Materials and Engineering · doi:10.1002/mame.201900620 · Cited by 17 · see citing works
- Authors
- Yang, B., Ayyadurai, N., Yun, H., Choi, Y. S., Hwang, B. H., Huang, J., Lu, Q., Zeng, H., & Cha, H. J.
- Affiliations
- Pohang University of Science and Technology (KR)
- Central Leather Research Institute (IN)
- Konkuk University (KR)
- Chungnam National University (KR)
- University of Alberta (CA)
- ORCID
- Venue
- Angewandte Chemie International Edition
- Publisher
- Wiley
- Published
- 2014-09-04
- Volume
- 53
- Issue
- 49
- Pages
- 13360-13364
- ISSN
- 1433-7851 (ISSN-L)
- 1521-3773
- OpenAlex
- W2164198916
- Cited by
- 121
- References
- 0
- Citations by year
- 2026: 5 · 2025: 11 · 2024: 13 · 2023: 7 · 2022: 11 · 2021: 15 · 2020: 8 · 2019: 19 · 2018: 11 · 2017: 10 · 2016: 6 · 2015: 4 · 2014: 1
- FWCI
- 2.76
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Angewandte Chemie International Edition (publishedVersion)
- PubMed (publishedVersion)
- Open Access System for Information Sharing (Pohang University of Science and Technology) (submittedVersion)
- Topics
- Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Keywords
- tyrosyl-tRNA synthetase, oxidative cross-linking, BioGlue, wet adhesion, water resistance, adhesive hydrogels, Escherichia coli
- MeSH terms
- Adhesiveness, Amino Acid Sequence, Animals, Dihydroxyphenylalanine, Escherichia coli, Molecular Sequence Data, Proteins, Recombinant Proteins, Water, Protein Engineering, Biomimetics, Bivalvia
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