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Label‐Free Molecular Imaging of Biological Cells and Tissues by Linear and Nonlinear Raman Spectroscopic Approaches
Abstract
Raman spectroscopy is an emerging technique in bioanalysis and imaging of biomaterials owing to its unique capability of generating spectroscopic fingerprints. Imaging cells and tissues by Raman microspectroscopy represents a nondestructive and label‐free approach. All components of cells or tissues contribute to the Raman signals, giving rise to complex spectral signatures. Resonance Raman scattering and surface‐enhanced Raman scattering can be used to enhance the signals and reduce the spectral complexity. Raman‐active labels can be introduced to increase specificity and multimodality. In addition, nonlinear coherent Raman scattering methods offer higher sensitivities, which enable the rapid imaging of larger sampling areas. Finally, fiber‐based imaging techniques pave the way towards in vivo applications of Raman spectroscopy. This Review summarizes the basic principles behind medical Raman imaging and its progress since 2012.
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The elements of statistical learning: Data mining, inference, and prediction
Franklin, J. (2005) · The Mathematical Intelligencer · doi:10.1007/bf02985802 · Cited by 1,400
Review of multidimensional data processing approaches for Raman and infrared spectroscopy
Gautam, R., Vanga, S., Ariese, F., & Umapathy, S. (2015) · EPJ Techniques and Instrumentation · doi:10.1140/epjti/s40485-015-0018-6 · Cited by 696
Spectral pre and post processing for infrared and Raman spectroscopy of biological tissues and cells
Byrne, H. J., Knief, P., Keating, M. E., & Bonnier, F. (2016) · Chemical Society Reviews · doi:10.1039/c5cs00440c · Cited by 213
A small animal Raman instrument for rapid, wide-area, spectroscopic imaging
Bohndiek, S. E., Wagadarikar, A., Zavaleta, C. L., Van de Sompel, D., Garai, E., Jokerst, J. V., Yazdanfar, S., & Gambhir, S. S. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1301379110 · Cited by 133
Darvin, M. E., Fluhr, J. W., Schanzer, S., Richter, H., Patzelt, A., Meinke, M. C., Zastrow, L., Golz, K., Doucet, O., Sterry, W., et al. (2011) · Journal of Dermatological Science · doi:10.1016/j.jdermsci.2011.06.009 · Cited by 57
Cited by
Cordero, E., Korinth, F., Stiebing, C., Krafft, C., Schie, I., & Popp, J. (2017) · Sensors · doi:10.3390/s17081724 · Cited by 58
Self-citationKorinth, F., Schmälzlin, E., Stiebing, C., Urrutia, T., Micheva, G., Sandin, C., Müller, A., Maiwald, M., Sumpf, B., Krafft, C., et al. (2020) · Sensors · doi:10.3390/s20236723 · Cited by 13
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Metadata
- Authors
- Krafft, C., Schmitt, M., Schie, I. W., Cialla‐May, D., Matthäus, C., Bocklitz, T., & Popp, J.
- Venue
- Angewandte Chemie International Edition
- Publisher
- Wiley
- Published
- 2017-03-31
- Volume
- 56
- Issue
- 16
- Pages
- 4392-4430
- ISSN
- 1433-7851 (ISSN-L)
- 1521-3773
- OpenAlex
- W2556890544
- Cited by
- 234
- References
- 5
- FWCI
- 13.37
- Type
- journal article
- Language
- en
- Open access
- closed
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