The DOI matches its Crossref record; 41 of the 41 references it cites were found in a registry or an index.
Assessment of cell engineering strategies for improved therapeutic protein production in CHO cells
Abstract
Recombinant glycoprotein therapeutics have proven to be invaluable pharmaceuticals for the treatment of various diseases. Chinese hamster ovary (CHO) cells are widely used in industry for the production of these proteins. Several strategies for engineering CHO cells for improved protein production have been tried with considerable results. The focus has mainly been to increase the specific productivity and to extend the culture longevity by preventing programmed cell death. These CHO cell engineering strategies, particularly those developed in Korea, are reviewed here.
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In the citing paper
“They have been employed by many groups as cell engineering tools but have given variable results (Mohan et al., 2008).”
From Therapeutic glycoprotein production in mammalian cells (2017) · cites this paper as Mohan et al., 2008
In the citing paper
“Similarly, the heavy chain-binding protein (BiP) chaperone gave mixed results, with enhanced or decreased productivity (Mohan et al., 2008).”
From Therapeutic glycoprotein production in mammalian cells (2017) · cites this paper as Mohan et al., 2008
In the citing paper
“Moreover, overexpression of the endoplasmic-reticulum ERp57 protein, an isoform of PDI, together with calnexin/calreticulin was able to increase thrombopoietin production in CHO cells (Mohan et al., 2008).”
From Therapeutic glycoprotein production in mammalian cells (2017) · cites this paper as Mohan et al., 2008
In the citing paper
“However, other studies observed either a negative or no effect on productivity (Mohan et al., 2008).”
From Therapeutic glycoprotein production in mammalian cells (2017) · cites this paper as Mohan et al., 2008
In the citing paper
“It has been suggested that translational and/or post-translational processes might also be rate-limiting for protein production, because of the lack of correlation between gene copy number and the amount of secreted proteins (Wilson et al., 1990;Mohan et al., 2008).”
From Therapeutic glycoprotein production in mammalian cells (2017) · cites this paper as Mohan et al., 2008
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Metadata
- Authors
- Mohan, C., Kim, Y., Koo, J., & Lee, G. M.
- Affiliations
- Korea Advanced Institute of Science and Technology (KR)
- Venue
- Biotechnology Journal
- Publisher
- Wiley
- Published
- 2008-05
- Volume
- 3
- Issue
- 5
- Pages
- 624-630
- ISSN
- 1860-6768 (ISSN-L)
- 1860-7314
- OpenAlex
- W2077304355
- Cited by
- 102
- References
- 41
- Citations by year
- 2025: 2 · 2024: 5 · 2023: 3 · 2022: 4 · 2021: 3 · 2020: 6 · 2019: 4 · 2018: 8 · 2017: 5 · 2016: 5 · 2015: 6 · 2014: 4 · 2013: 12 · 2012: 9
- FWCI
- 0.67
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biotechnology Journal (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein purification and stability
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Transgenic Plants and Applications
- Keywords
- CHO cell engineering, therapeutic proteins, specific productivity, culture longevity, programmed cell death, CHO cells, bioprocess optimization
- MeSH terms
- Animals, Cricetulus, Cricetinae, Recombinant Proteins, Technology Assessment, Biomedical, Protein Engineering, CHO Cells, Genetic Enhancement
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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