The DOI matches its Crossref record; 41 of the 41 references it cites were found in a registry or an index.
Assessment of cell engineering strategies for improved therapeutic protein production in CHO cells
Abstract
Recombinant glycoprotein therapeutics have proven to be invaluable pharmaceuticals for the treatment of various diseases. Chinese hamster ovary (CHO) cells are widely used in industry for the production of these proteins. Several strategies for engineering CHO cells for improved protein production have been tried with considerable results. The focus has mainly been to increase the specific productivity and to extend the culture longevity by preventing programmed cell death. These CHO cell engineering strategies, particularly those developed in Korea, are reviewed here.
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In the citing paper
“They have been employed by many groups as cell engineering tools but have given variable results (Mohan et al., 2008).”
From Therapeutic glycoprotein production in mammalian cells (2017) · cites this paper as Mohan et al., 2008
In the citing paper
“Similarly, the heavy chain-binding protein (BiP) chaperone gave mixed results, with enhanced or decreased productivity (Mohan et al., 2008).”
From Therapeutic glycoprotein production in mammalian cells (2017) · cites this paper as Mohan et al., 2008
In the citing paper
“Moreover, overexpression of the endoplasmic-reticulum ERp57 protein, an isoform of PDI, together with calnexin/calreticulin was able to increase thrombopoietin production in CHO cells (Mohan et al., 2008).”
From Therapeutic glycoprotein production in mammalian cells (2017) · cites this paper as Mohan et al., 2008
In the citing paper
“However, other studies observed either a negative or no effect on productivity (Mohan et al., 2008).”
From Therapeutic glycoprotein production in mammalian cells (2017) · cites this paper as Mohan et al., 2008
In the citing paper
“It has been suggested that translational and/or post-translational processes might also be rate-limiting for protein production, because of the lack of correlation between gene copy number and the amount of secreted proteins (Wilson et al., 1990;Mohan et al., 2008).”
From Therapeutic glycoprotein production in mammalian cells (2017) · cites this paper as Mohan et al., 2008
Citations
Cites
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Effect of solution pH on protein transport through ultrafiltration membranes
Burns, D. B., & Zydney, A. L. (1999) · Biotechnology and Bioengineering · doi:10.1002/(sici)1097-0290(19990705)64:1 · Cited by 122
Nutrient deprivation induces autophagy as well as apoptosis in Chinese hamster ovary cell culture
Hwang, S. O., & Lee, G. M. (2007) · Biotechnology and Bioengineering · doi:10.1002/bit.21589 · Cited by 112
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Research works printed but not linked: 2
- 10.1016/S0168-1656(02)00011-1 · DOI
- Chung J. Y. Development of recombinant Chinese hamster ovary cell lines producing human thrombopoietin analogs.PhD thesis Department of Biological Sciences KAIST Daejeon Korea 2002.
Cited by
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Items 101–101 of 101
Sheikholeslami, Z. (2013) · PolyPublie (École Polytechnique de Montréal)
Metadata
- Authors
- Mohan, C., Kim, Y., Koo, J., & Lee, G. M.
- Affiliations
- Korea Advanced Institute of Science and Technology (KR)
- Venue
- Biotechnology Journal
- Publisher
- Wiley
- Published
- 2008-05
- Volume
- 3
- Issue
- 5
- Pages
- 624-630
- ISSN
- 1860-6768 (ISSN-L)
- 1860-7314
- OpenAlex
- W2077304355
- Cited by
- 102
- References
- 41
- Citations by year
- 2025: 2 · 2024: 5 · 2023: 3 · 2022: 4 · 2021: 3 · 2020: 6 · 2019: 4 · 2018: 8 · 2017: 5 · 2016: 5 · 2015: 6 · 2014: 4 · 2013: 12 · 2012: 9
- FWCI
- 0.67
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biotechnology Journal (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein purification and stability
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Transgenic Plants and Applications
- Keywords
- CHO cell engineering, therapeutic proteins, specific productivity, culture longevity, programmed cell death, CHO cells, bioprocess optimization
- MeSH terms
- Animals, Cricetulus, Cricetinae, Recombinant Proteins, Technology Assessment, Biomedical, Protein Engineering, CHO Cells, Genetic Enhancement
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