The DOI matches its Crossref record; 13 of the 13 references it cites were found in a registry or an index.
Optimization of cardiac cell seeding and distribution in 3D porous alginate scaffolds
Abstract
Cardiac tissue engineering has evolved as a potential therapeutic approach to assist in cardiac regeneration. We have recently shown that tissue‐engineered cardiac graft, constructed from cardiomyocytes seeded within an alginate scaffold, is capable of preventing the deterioration in cardiac function after myocardial infarction in rats. The present article addresses cell seeding within porous alginate scaffolds in an attempt to achieve 3D high‐density cardiac constructs with a uniform cell distribution. Due to the hydrophilic nature of the alginate scaffold, its >90% porosity and interconnected pore structure, cell seeding onto the scaffold was efficient and short, up to 30 min. Application of a moderate centrifugal force during cell seeding resulted in a uniform cell distribution throughout the alginate scaffolds, consequently enabling the loading of a large number of cells onto the 3D scaffolds. The percent cell yield in the alginate scaffolds ranged between 60–90%, depending on cell density at seeding; it was 90% at seeding densities of up to 1 × 10 8 cells/cm 3 scaffold and decreased to 60% at higher densities. The highly dense cardiac constructs maintained high metabolic activity in culture. Scanning electron microscopy revealed that the cells aggregated within the scaffold pores. Some of the aggregates were contracting spontaneously within the matrix pores. Throughout the culture there was no indication of cardiomyocyte proliferation within the scaffolds, nor was it found in 3D cultures of cardiofibroblasts. This may enable the development of cardiac cocultures, without domination of cardiofibroblasts with time. © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 80: 305–312, 2002.
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Bioengineered Cardiac Grafts : A New Approach to Repair the Infarcted Myocardium?
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Self-citationCarrier, R. L., Papadaki, M., Rupnick, M., Schoen, F. J., Bursac, N., Langer, R., Freed, L. E., & Vunjak-Novakovic, G. (1999) · Biotechnology and Bioengineering · doi:10.1002/(sici)1097-0290(19990905)64:5<580::aid-bit8>3.0.co;2-x · Cited by 540
Novel alginate sponges for cell culture and transplantation
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Hepatocyte behavior within three-dimensional porous alginate scaffolds
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Self-citationPapadaki, M., Bursac, N., Langer, R., Merok, J., Vunjak-Novakovic, G., & Freed, L. E. (2001) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.2001.280.1.h168 · Cited by 284
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Construction of a bioengineered cardiac graft
Li, R.-K., Yau, T. M., Weisel, R. D., Mickle, D. A. G., Sakai, T., Choi, A., & Jia, Z.-Q. (2000) · The Journal of Thoracic and Cardiovascular Surgery · doi:10.1016/s0022-5223(00)70193-0 · Cited by 208
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Growth of engineered human myocardium with mechanical loading and vascular coculture
Tulloch, N. L., Muskheli, V., Razumova, M. V., Korte, F. S., Regnier, M., Hauch, K. D., Pabon, L., Reinecke, H., & Murry, C. E. (2011) · Circulation Research · doi:10.1161/circresaha.110.237206 · Cited by 666
3D Cell Culture in Alginate Hydrogels
Andersen, T., Auk-Emblem, P., & Dornish, M. (2015) · Microarrays · doi:10.3390/microarrays4020133 · Cited by 472
Ruan, J.-L., Tulloch, N. L., Razumova, M. V., Saiget, M., Muskheli, V., Pabon, L., Reinecke, H., Regnier, M., & Murry, C. E. (2016) · Circulation · doi:10.1161/circulationaha.114.014998 · Cited by 467
Prevascularization of cardiac patch on the omentum improves its therapeutic outcome
Dvir, T., Kedem, A., Ruvinov, E., Levy, O., Freeman, I., Landa, N., Holbova, R., Feinberg, M. S., Dror, S., Etzion, Y., et al. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0812242106 · Cited by 345
Self-citationRuvinov, E., & Cohen, S. (2016) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2015.04.021 · Cited by 303
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Metadata
- Authors
- Dar, A., Shachar, M., Leor, J., & Cohen, S.
- Venue
- Biotechnology and Bioengineering
- Publisher
- Wiley
- Published
- 2002-09-10
- Volume
- 80
- Issue
- 3
- Pages
- 305-312
- ISSN
- 0006-3592 (ISSN-L)
- 1097-0290
- OpenAlex
- W2164803358
- Cited by
- 384
- References
- 13
- FWCI
- 12.40
- Type
- journal article
- Language
- en
- Open access
- closed
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