The DOI matches its Crossref record; 115 of the 115 references it cites were found in a registry or an index.
3D Cell Culture in Alginate Hydrogels
Open accessAbstract
This review compiles information regarding the use of alginate, and in particular alginate hydrogels, in culturing cells in 3D. Knowledge of alginate chemical structure and functionality are shown to be important parameters in design of alginate-based matrices for cell culture. Gel elasticity as well as hydrogel stability can be impacted by the type of alginate used, its concentration, the choice of gelation technique (ionic or covalent), and divalent cation chosen as the gel inducing ion. The use of peptide-coupled alginate can control cell–matrix interactions. Gelation of alginate with concomitant immobilization of cells can take various forms. Droplets or beads have been utilized since the 1980s for immobilizing cells. Newer matrices such as macroporous scaffolds are now entering the 3D cell culture product market. Finally, delayed gelling, injectable, alginate systems show utility in the translation of in vitro cell culture to in vivo tissue engineering applications. Alginate has a history and a future in 3D cell culture. Historically, cells were encapsulated in alginate droplets cross-linked with calcium for the development of artificial organs. Now, several commercial products based on alginate are being used as 3D cell culture systems that also demonstrate the possibility of replacing or regenerating tissue.
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Cited by
Unagolla, J. M., & Jayasuriya, A. C. (2020) · Applied Materials Today · doi:10.1016/j.apmt.2019.100479 · Cited by 544
Alginate and its application to tissue engineering
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“Other materials that focus on utilizing biopolymers for material backbone, such as polysaccharides and glycosaminoglycans (GAGs), have been reviewed extensively by others (Andersen et al., 2015; Custódio et al., 2014; Lutolf and Hubbell, 2005; Van Vlierberghe et al., 2011).”
Background
Metadata
- Authors
- Andersen, T., Auk-Emblem, P., & Dornish, M.
- Venue
- Microarrays
- Publisher
- MDPI AG
- Published
- 2015-03-24
- Volume
- 4
- Issue
- 2
- Pages
- 133-161
- ISSN
- 2076-3905 (ISSN-L)
- OpenAlex
- W2055699288
- Cited by
- 472
- References
- 115
- FWCI
- 8.64
- Type
- journal article
- Language
- en
- Open access
- gold
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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