The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.
A new, mild cross‐linking methodology to prepare cross‐linked enzyme aggregates
Abstract
Cross‐linked enzyme aggregates (CLEAs) were prepared from several enzymes (penicillin G acylase, hydroxynitrile lyase, alcohol dehydrogenase, and two different nitrilases) by precipitation and subsequent cross‐linking using dextran polyaldehyde. In most cases, higher immobilization yields were obtained using the latter cross‐linker as compared with the commonly used glutaraldehyde. Active site titration of penicillin acylase CLEAs showed that the higher activity originated from a significantly lower loss in active sites using dextran polyaldehyde as a cross‐linking agent. It is proposed that macromolecular cross‐linkers are too large to penetrate the protein active site and react with catalytically essential amino acid residues. © 2004 Wiley Periodicals, Inc.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302
Aldehyde-agarose gels as activated supports for immobilization-stabilization of enzymes
Guisán, J. (1988) · Enzyme and Microbial Technology · doi:10.1016/0141-0229(88)90018-x · Cited by 602
Cao, L., van Rantwijk, F., & Sheldon, R. A. (2000) · Organic Letters · doi:10.1021/ol005593x · Cited by 472
Self-citationPalomo, J. M., Muñoz, G., Fernández-Lorente, G., Mateo, C., Fernández-Lafuente, R., & Guisán, J. M. (2002) · Journal of Molecular Catalysis B: Enzymatic · doi:10.1016/s1381-1177(02)00178-9 · Cited by 443
Self-citationINTERMOLECULAR CROSS LINKING OF A PROTEIN IN THE CRYSTALLINE STATE: CARBOXYPEPTIDASE-A
Quiocho, F. A., & Richards, F. M. (1964) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.52.3.833 · Cited by 292
Cross-linked enzyme crystals as robust biocatalysts
St. Clair, N. L., & Navia, M. A. (1992) · Journal of the American Chemical Society · doi:10.1021/ja00044a064 · Cited by 283
Cross-linked enzyme aggregates with enhanced activity: application to lipases
López-Serrano, P., Cao, L., van Rantwijk, F., & Sheldon, R. A. (2002) · Biotechnology Letters · doi:10.1023/a:1019863314646 · Cited by 263
Self-citationPreparation of enzymically active, water-insoluble derivatives of trypsin
Habeeb, A. F. S. A. (1967) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(67)90453-5 · Cited by 154
Cao, L., van Langen, L. M., van Rantwijk, F., & Sheldon, R. A. (2001) · Journal of Molecular Catalysis B Enzymatic · doi:10.1016/s1381-1177(00)00078-3 · Cited by 141
Self-citationPace, H. C., Hodawadekar, S. C., Draganescu, A., Huang, J., Bieganowski, P., Pekarsky, Y., Croce, C. M., & Brenner, C. (2000) · Current Biology · doi:10.1016/s0960-9822(00)00621-7 · Cited by 135
Stabilization of multimeric enzymes via immobilization and post-immobilization techniques
Fernández‐Lafuente, R., Rodriguez, V., Mateo, C., Penzol, G., Hernández-Jústiz, O., Irazoqui, G., Villarino, A., Ovsejevi, K., Batista, F., & Guisan, J. M. (1999) · Journal of Molecular Catalysis B Enzymatic · doi:10.1016/s1381-1177(99)00028-4 · Cited by 125
Self-citationFernández‐Lafuente, R., Rosell, C. M., Canaán‐Haden, L., Rodés, L., & Guisan, J. M. (1999) · Enzyme and Microbial Technology · doi:10.1016/s0141-0229(98)00102-1 · Cited by 99
Torres, R. G., Mateo, C., Fuentes, M., et al. (2002) · Biotechnology Progress · doi:10.1021/bp020082q · Cited by 81
Self-citationvan Langen, L. M., Janssen, M. H. A., Oosthoek, N. H. P., Pereira, S. M., Švedas, V. K., van Rantwijk, F., & Sheldon, R. A. (2002) · Biotechnology and Bioengineering · doi:10.1002/bit.10280 · Cited by 58
Self-citation
Cited by
Enzyme immobilisation in biocatalysis: why, what and how
Sheldon, R. A., & van Pelt, S. (2013) · Chem. Soc. Rev. · doi:10.1039/c3cs60075k · Cited by 2,675
Self-citationEnzyme Immobilization: The Quest for Optimum Performance
Sheldon, R. A. (2007) · Advanced Synthesis & Catalysis · doi:10.1002/adsc.200700082 · Cited by 2,077
Self-citationImmobilization of enzymes on porous silicas – benefits and challenges
Hartmann, M., & Kostrov, X. (2013) · Chemical Society Reviews · doi:10.1039/c3cs60021a · Cited by 605
Cross-linked enzyme aggregates (CLEA®s): stable and recyclable biocatalysts
Sheldon, R. A. (2007) · Biochemical Society Transactions · doi:10.1042/bst0351583 · Cited by 422
Self-citationMethods for stabilizing and activating enzymes in ionic liquids—a review
Zhao, H. (2010) · Journal of Chemical Technology & Biotechnology · doi:10.1002/jctb.2375 · Cited by 351
Metadata
- Authors
- Mateo, C., Palomo, J. M., van Langen, L. M., van Rantwijk, F., & Sheldon, R. A.
- Venue
- Biotechnology and Bioengineering
- Publisher
- Wiley
- Published
- 2004-03-11
- Volume
- 86
- Issue
- 3
- Pages
- 273-276
- ISSN
- 0006-3592 (ISSN-L)
- 1097-0290
- OpenAlex
- W2121758307
- Cited by
- 300
- References
- 14
- FWCI
- 2.73
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
