The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
Continuous perfusion microfluidic cell culture array for high‐throughput cell‐based assays
Open accessAbstract
We present for the first time a microfluidic cell culture array for long‐term cellular monitoring. The 10 × 10 array could potentially assay 100 different cell‐based experiments in parallel. The device was designed to integrate the processes used in typical cell culture experiments on a single self‐contained microfluidic system. Major functions include repeated cell growth/passage cycles, reagent introduction, and real‐time optical analysis. The single unit of the array consists of a circular microfluidic chamber, multiple narrow perfusion channels surrounding the main chamber, and four ports for fluidic access. Human carcinoma (HeLa) cells were cultured inside the device with continuous perfusion of medium at 37°C. The observed doubling time was 1.4 ± 0.1 days with a peak cell density of ∼2.5*10 5 cells/cm 2. Cell assay was demonstrated by monitoring the fluorescence localization of calcein AM from 1 min to 10 days after reagent introduction. Confluent cell cultures were passaged within the microfluidic chambers using trypsin and successfully regrown, suggesting a stable culture environment suitable for continuous operation. The cell culture array could offer a platform for a wide range of assays with applications in drug screening, bioinformatics, and quantitative cell biology. © 2004 Wiley Periodicals, Inc.
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Metadata
- Authors
- Hung, P. J., Lee, P. J., Sabounchi, P., Lin, R., & Lee, L. P.
- Venue
- Biotechnology and Bioengineering
- Publisher
- Wiley
- Published
- 2004-12-03
- Volume
- 89
- Issue
- 1
- Pages
- 1-8
- ISSN
- 0006-3592 (ISSN-L)
- 1097-0290
- OpenAlex
- W2004441496
- Cited by
- 522
- References
- 20
- FWCI
- 12.11
- Type
- journal article
- Language
- en
- Open access
- bronze
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
