Drug susceptibility of matrix‐encapsulated Candida albicans nano‐biofilms
Open accessAbstract
The rise in the use of biomedical devices and implants has seen a concomitant surge in the advent of device‐related nosocomial (hospital‐acquired) infections of bacterial and fungal origins. The most common nosocomial fungal infection is candidiasis caused mainly by Candida albicans biofilms. Candidiasis is associated with an unacceptably high mortality rate, and there is an urgent need for the discovery of new antifungal drugs that prevent or control biofilm formation. To this end, we recently developed an ultra‐high‐throughput microarray platform consisting of nano‐scale biofilms of C. albicans encapsulated in collagen or alginate hydrogel matrices for antifungal drug screening. Here, we report that the choice of matrix influences the apparent susceptibility of C. albicans to the common antifungal drugs, amphotericin B, and caspofungin. While amphotericin B is equally effective against biofilms grown in collagen and alginate matrices, caspofungin is effective only against biofilms grown only in alginate, but not in collagen. We demonstrate differences in the distribution of the drugs in the two matrices may contribute to the susceptibility of C. albicans nano‐biofilms. In a larger context, our results highlight the importance of the choice of matrix as a parameter in 3D cell encapsulation, and suggest a screening strategy to predict drug performance in vivo. Biotechnol. Bioeng. 2014;111: 418–424. © 2013 Wiley Periodicals, Inc.
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Screens of chemical libraries have identified compounds that disrupt formation and/or maintenance of biofilms.
“Screens of chemical libraries have identified compounds that disrupt formation and/or maintenance of biofilms (25, 101, 185, 210).”
From <i>Candida albicans</i> Biofilms and Human Disease (2015) · cites this paper as [185]
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- Authors
- Srinivasan, A., Gupta, C. M., Agrawal, C. M., Leung, K. P., Lopez‐Ribot, J. L., & Ramasubramanian, A. K.
- Affiliations
- The University of Texas at San Antonio (US)
- United States Army Institute of Surgical Research (US)
- Department of Biology The University of Texas at San Antonio San Antonio Texas
- Corresponding author
- Ramasubramanian, Anand K.
- Venue
- Biotechnology and Bioengineering
- Publisher
- Wiley
- Published
- 2013-10-18
- Volume
- 111
- Issue
- 2
- Pages
- 418-424
- ISSN
- 0006-3592 (ISSN-L)
- 1097-0290
- PMC
- PMC4331649
- OpenAlex
- W2132776307
- Cited by
- 11
- References
- 32
- Citations by year
- 2021: 1 · 2019: 2 · 2018: 1 · 2017: 2 · 2016: 3 · 2015: 2
- FWCI
- 1.22
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Biotechnology and Bioengineering (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (R01DE023510, SC1HL112629, G12RR01 3646)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- Candida albicans, fungal biofilms, antifungal susceptibility, amphotericin B, caspofungin, collagen gel, alginate hydrogel, 3D cell encapsulation, antifungal drug screening, microarray platforms, device-related infection, candidiasis
- MeSH terms
- Caspofungin, Amphotericin B, Antifungal Agents, Candida albicans, Drug Evaluation, Preclinical, Microbial Sensitivity Tests, Biofilms, Cells, Immobilized, Hydrogel, Polyethylene Glycol Dimethacrylate, Echinocandins, Lipopeptides
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