The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
In vitro reconstitution of mevalonate pathway and targeted engineering of farnesene overproduction in Escherichia coli
Abstract
Approaches using metabolic engineering and synthetic biology to overproduce terpenoids, such as the precursors of taxol and artemisinin, in microbial systems have achieved initial success. However, due to the lack of steady‐state kinetic information and incomplete understanding of the terpenoid biosynthetic pathway, it has been difficult to build a highly efficient, universal system. Here, we reconstituted the mevalonate pathway to produce farnesene (a precursor of new jet fuel) in vitro using purified protein components. The information from this in vitro reconstituted system guided us to rationally optimize farnesene production in E. coli by quantitatively overexpressing each component. Targeted proteomic assays and intermediate assays were used to determine the metabolic status of each mutant. Through targeted engineering, farnesene production could be increased predictably step by step, up to 1.1 g/L (∼2,000 fold) 96 h after induction at the shake‐flask scale. The strategy developed to release the potential of the mevalonate pathway for terpenoid overproduction should also work in other multistep synthetic pathways. Biotechnol. Bioeng. 2014;111: 1396–1405. © 2014 Wiley Periodicals, Inc.
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Cites
Regulation of the mevalonate pathway
Goldstein, J. L., & Brown, M. S. (1990) · Nature · doi:10.1038/343425a0 · Cited by 5,394
High-level semi-synthetic production of the potent antimalarial artemisinin
Paddon, C. J., Westfall, P. J., Pitera, D. J., Benjamin, K., Fisher, K., McPhee, D., Leavell, M. D., Tai, A., Main, A., Eng, D., et al. (2013) · Nature · doi:10.1038/nature12051 · Cited by 2,060
Programming cells by multiplex genome engineering and accelerated evolution
Wang, H. H., Isaacs, F. J., Carr, P. A., Sun, Z. Z., Xu, G., Forest, C. R., & Church, G. M. (2009) · Nature · doi:10.1038/nature08187 · Cited by 1,529 · cited 1×
“These achievements, however, were based on traditional labor-intensive combinations of molecular cloning or high throughput screening method (Dietrich et al., 2010; Wang et al., 2009).”
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Wood-Derived Materials for Green Electronics, Biological Devices, and Energy Applications
Zhu, H., Luo, W., Ciesielski, P. N., Fang, Z., Zhu, J. Y., Henriksson, G., Himmel, M. E., & Hu, L. (2016) · Chemical Reviews · doi:10.1021/acs.chemrev.6b00225 · Cited by 1,532
Renewable bio-jet fuel production for aviation: a review
Wei, H., Liu, W., Chen, X., Yang, Q., Li, J., & Chen, H. (2019) · Fuel · doi:10.1016/j.fuel.2019.06.007 · Cited by 435
Multienzyme Cascade Reactions—Status and Recent Advances
Sperl, J. M., & Sieber, V. (2018) · ACS Catalysis · doi:10.1021/acscatal.7b03440 · Cited by 363
Advances and prospects in metabolic engineering of Zymomonas mobilis
Wang, X., He, Q., Yang, Y., Wang, J., Haning, K., Hu, Y., Wu, B., He, M., Zhang, Y., Bao, J., et al. (2018) · Metabolic Engineering · doi:10.1016/j.ymben.2018.04.001 · Cited by 154
Renewable production of high density jet fuel precursor sesquiterpenes from Escherichia coli
Liu, C.-L., Tian, T., Alonso-Gutierrez, J., Garabedian, B., Wang, S., Baidoo, E. E. K., Benites, V., Chen, Y., Petzold, C. J., Adams, P. D., et al. (2018) · Biotechnology for Biofuels · doi:10.1186/s13068-018-1272-z · Cited by 70
Metadata
- Authors
- Zhu, F., Zhong, X., Hu, M., Lu, L., Deng, Z., & Liu, T.
- Venue
- Biotechnology and Bioengineering
- Publisher
- Wiley
- Published
- 2014-02-17
- Volume
- 111
- Issue
- 7
- Pages
- 1396-1405
- ISSN
- 0006-3592 (ISSN-L)
- 1097-0290
- OpenAlex
- W1900473455
- Cited by
- 203
- References
- 3
- FWCI
- 7.06
- Type
- journal article
- Language
- en
- Open access
- closed
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